Team:Michigan/Notebook/Protocols
From 2012.igem.org
(Difference between revisions)
Line 8: | Line 8: | ||
</h3> | </h3> | ||
<h2>General</h2> | <h2>General</h2> | ||
- | |||
Plate Production</html>[https://static.igem.org/mediawiki/2012/0/08/MichPlate.pdf]<html> | Plate Production</html>[https://static.igem.org/mediawiki/2012/0/08/MichPlate.pdf]<html> | ||
Latest revision as of 02:26, 4 October 2012
Protocols
General
Plate Production[1]Cloning
Removing BioBricks from the Registry[2]Production of Competent Cells[3]
Heat Shock Transformation[4]
DNA Purification: PCR[5] DNA Purification: MiniPrep[6]
Restriction Enzyme Digest[7]
Ligation[8]
PCR (Phusion)[9]
Colony PCR[10]
Gel Electrophoresis[11]
Characterization
Transmission Electron Microscopy Protocol[12]
FimE Asymmetrical Digest Assay Protocol[13]