Team:Michigan/Notebook/Protocols

From 2012.igem.org

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<h2>General</h2>
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Plate Production</html>[https://static.igem.org/mediawiki/2012/0/08/MichPlate.pdf]<html>
Plate Production</html>[https://static.igem.org/mediawiki/2012/0/08/MichPlate.pdf]<html>
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<h2>Characterization</h2>
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Transmission Electron Microscopy Protocol
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Transmission Electron Microscopy Protocol</html>[https://static.igem.org/mediawiki/2012/0/0a/MichTEM.pdf]<html>
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FimE Asymmetrical Digest Assay Protocol</html>[https://static.igem.org/mediawiki/2012/a/aa/MichAsm.pdf]<html>
FimE Asymmetrical Digest Assay Protocol</html>[https://static.igem.org/mediawiki/2012/a/aa/MichAsm.pdf]<html>

Latest revision as of 02:26, 4 October 2012

Xayona Website Template

Protocols

General

Plate Production[1]

Cloning

Removing BioBricks from the Registry[2]

Production of Competent Cells[3]

Heat Shock Transformation[4]

DNA Purification: PCR[5] DNA Purification: MiniPrep[6]

Restriction Enzyme Digest[7]

Ligation[8]

PCR (Phusion)[9]

Colony PCR[10]

Gel Electrophoresis[11]

Characterization


Transmission Electron Microscopy Protocol[12]

FimE Asymmetrical Digest Assay Protocol[13]