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Team:Michigan/Notebook/Protocols
From 2012.igem.org
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<h2>General</h2> | <h2>General</h2> | ||
| - | </html> | + | Plate Production</html>[https://static.igem.org/mediawiki/2012/0/08/MichPlate.pdf]<html> |
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<html> | <html> | ||
<h2>Cloning</h2> | <h2>Cloning</h2> | ||
| - | Removing BioBricks from the Registry | + | Removing BioBricks from the Registry</html>[https://static.igem.org/mediawiki/2012/5/50/MichRem.pdf]<html> |
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<br><br> | <br><br> | ||
| - | Production of Competent Cells | + | Production of Competent Cells</html>[https://static.igem.org/mediawiki/2012/6/61/MichComp.pdf]<html> |
<br><br> | <br><br> | ||
| - | Heat Shock Transformation | + | Heat Shock Transformation</html>[https://static.igem.org/mediawiki/2012/0/0e/MichHeat.pdf]<html> |
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<br><br> | <br><br> | ||
| - | DNA Purification | + | DNA Purification: PCR</html>[https://static.igem.org/mediawiki/2012/0/04/MichPur2.pdf]<html> |
| + | DNA Purification: MiniPrep</html>[https://static.igem.org/mediawiki/2012/1/19/MichPur1.pdf]<html> | ||
<br><br> | <br><br> | ||
| - | Restriction Enzyme Digest | + | Restriction Enzyme Digest</html>[https://static.igem.org/mediawiki/2012/0/0f/MichRestrict.pdf]<html> |
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<br><br> | <br><br> | ||
| - | Ligation | + | Ligation</html>[https://static.igem.org/mediawiki/2012/1/10/MichLig.pdf]<html> |
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<br><br> | <br><br> | ||
| - | PCR (Phusion) | + | PCR (Phusion)</html>[https://static.igem.org/mediawiki/2012/9/94/MichPCR.pdf]<html> |
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<br><br> | <br><br> | ||
| - | Colony PCR | + | Colony PCR</html>[https://static.igem.org/mediawiki/2012/9/96/MichCol.pdf]<html> |
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<br><br> | <br><br> | ||
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| + | Gel Electrophoresis</html>[https://static.igem.org/mediawiki/2012/1/12/MichElectro.pdf]<html> | ||
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| + | <br><br> | ||
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<h2>Characterization</h2> | <h2>Characterization</h2> | ||
<br> | <br> | ||
| - | Transmission Electron Microscopy Protocol | + | Transmission Electron Microscopy Protocol</html>[https://static.igem.org/mediawiki/2012/0/0a/MichTEM.pdf]<html> |
<br><br> | <br><br> | ||
| - | FimE Asymmetrical Digest Assay Protocol | + | FimE Asymmetrical Digest Assay Protocol</html>[https://static.igem.org/mediawiki/2012/a/aa/MichAsm.pdf]<html> |
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</div> | </div> | ||
</div> | </div> | ||
</html> | </html> | ||
Latest revision as of 02:26, 4 October 2012
Protocols
General
Plate Production[1]Cloning
Removing BioBricks from the Registry[2]Production of Competent Cells[3]
Heat Shock Transformation[4]
DNA Purification: PCR[5] DNA Purification: MiniPrep[6]
Restriction Enzyme Digest[7]
Ligation[8]
PCR (Phusion)[9]
Colony PCR[10]
Gel Electrophoresis[11]
Characterization
Transmission Electron Microscopy Protocol[12]
FimE Asymmetrical Digest Assay Protocol[13]
