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Team:Michigan/Notebook/Protocols
From 2012.igem.org
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<h2>Cloning</h2> | <h2>Cloning</h2> | ||
| - | Removing BioBricks from the Registry[https://static.igem.org/mediawiki/2012/5/50/MichRem.pdf] | + | Removing BioBricks from the Registry</html>[https://static.igem.org/mediawiki/2012/5/50/MichRem.pdf]<html> |
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Revision as of 02:24, 4 October 2012
Protocols
General
Plate Production</html>[1]Cloning
Removing BioBricks from the Registry[2]Production of Competent Cells[3]
Heat Shock Transformation[4]
DNA Purification: PCR[5] DNA Purification: MiniPrep[6]
Restriction Enzyme Digest[7]
Ligation[8]
PCR (Phusion)[9]
Colony PCR[10]
Gel Electrophoresis[11]
Characterization
Transmission Electron Microscopy Protocol
FimE Asymmetrical Digest Assay Protocol[12]
