Team:Michigan/Notebook/Protocols

From 2012.igem.org

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Revision as of 02:23, 4 October 2012

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Protocols

General

Plate Production</html>[1]

Cloning

Removing BioBricks from the Registry[https://static.igem.org/mediawiki/2012/5/50/MichRem.pdf]

Production of Competent Cells[2]

Heat Shock Transformation[3]

DNA Purification: PCR[4] DNA Purification: MiniPrep[5]

Restriction Enzyme Digest[6]

Ligation[7]

PCR (Phusion)[8]

Colony PCR[9]

Gel Electrophoresis[10]

Characterization

Transmission Electron Microscopy Protocol

FimE Asymmetrical Digest Assay Protocol[11]

@@ Line 9: / Line 9: @@
 <h2>General</h2>
 </html>
-Plate Production
+Plate Production</html>[https://static.igem.org/mediawiki/2012/0/08/MichPlate.pdf]<html>
 <html>
 <h2>Cloning</h2>
-Removing BioBricks from the Registry
+Removing BioBricks from the Registry[https://static.igem.org/mediawiki/2012/5/50/MichRem.pdf]
 <br><br>
-Production of Competent Cells
+Production of Competent Cells</html>[https://static.igem.org/mediawiki/2012/6/61/MichComp.pdf]<html>
 <br><br>
-Heat Shock Transformation
+Heat Shock Transformation</html>[https://static.igem.org/mediawiki/2012/0/0e/MichHeat.pdf]<html>
 <br><br>
-DNA Purification
+DNA Purification: PCR</html>[https://static.igem.org/mediawiki/2012/0/04/MichPur2.pdf]<html>
+DNA Purification: MiniPrep</html>[https://static.igem.org/mediawiki/2012/1/19/MichPur1.pdf]<html>
 <br><br>
-Restriction Enzyme Digest
+Restriction Enzyme Digest</html>[https://static.igem.org/mediawiki/2012/0/0f/MichRestrict.pdf]<html>
 <br><br>
-Ligation
+Ligation</html>[https://static.igem.org/mediawiki/2012/1/10/MichLig.pdf]<html>
 <br><br>
-PCR (Phusion)
+PCR (Phusion)</html>[https://static.igem.org/mediawiki/2012/9/94/MichPCR.pdf]<html>
 <br><br>
-Colony PCR
+Colony PCR</html>[https://static.igem.org/mediawiki/2012/9/96/MichCol.pdf]<html>
 <br><br>
+Gel Electrophoresis</html>[https://static.igem.org/mediawiki/2012/1/12/MichElectro.pdf]<html>
+<br><br>
 <h2>Characterization</h2>
 <br>
 Transmission Electron Microscopy Protocol
 <br><br>
-FimE Asymmetrical Digest Assay Protocol
+FimE Asymmetrical Digest Assay Protocol</html>[https://static.igem.org/mediawiki/2012/a/aa/MichAsm.pdf]<html>
 </div>
 </div>
 </html>
-[[File:MichRem.pdf]]
-https://static.igem.org/mediawiki/2012/5/50/MichRem.pdf
-[[File:MichElectro.pdf]]
-https://static.igem.org/mediawiki/2012/1/12/MichElectro.pdf
-[[File:MichHeat.pdf]]
-https://static.igem.org/mediawiki/2012/0/0e/MichHeat.pdf
-[[File:MichLig.pdf]]
-https://static.igem.org/mediawiki/2012/1/10/MichLig.pdf
-[[File:MichPCR.pdf]]
-https://static.igem.org/mediawiki/2012/9/94/MichPCR.pdf
-[[File:MichCol.pdf]]
-https://static.igem.org/mediawiki/2012/9/96/MichCol.pdf
-[[File:MichPlate.pdf]]
-https://static.igem.org/mediawiki/2012/0/08/MichPlate.pdf
-[[File:MichTEM.pdf]]
-[[File:MichAsm.pdf]]
-https://static.igem.org/mediawiki/2012/a/aa/MichAsm.pdf
-[[File:MichPur1.pdf]]
-https://static.igem.org/mediawiki/2012/1/19/MichPur1.pdf
-[[File:MichPur2.pdf]]
-https://static.igem.org/mediawiki/2012/0/04/MichPur2.pdf
-[[File:MichRestrict.pdf]]
-https://static.igem.org/mediawiki/2012/0/0f/MichRestrict.pdf
-[[File:MichComp.pdf]]
-https://static.igem.org/mediawiki/2012/6/61/MichComp.pdf