"
Team:Michigan/Notebook/Protocols
From 2012.igem.org
(Difference between revisions)
| Line 12: | Line 12: | ||
<html> | <html> | ||
<h2>Cloning</h2> | <h2>Cloning</h2> | ||
| - | + | ||
| - | Removing BioBricks from the Registry | + | Removing BioBricks from the Registry |
| - | Production of Competent Cells | + | <br><br> |
| + | Production of Competent Cells | ||
| + | <br><br> | ||
Heat Shock Transformation | Heat Shock Transformation | ||
| + | <br><br> | ||
DNA Purification | DNA Purification | ||
| + | <br><br> | ||
Restriction Enzyme Digest | Restriction Enzyme Digest | ||
| + | <br><br> | ||
Ligation | Ligation | ||
| + | <br><br> | ||
PCR (Phusion) | PCR (Phusion) | ||
| + | <br><br> | ||
Colony PCR | Colony PCR | ||
| - | + | <br><br> | |
| - | < | + | |
<h2>Characterization</h2> | <h2>Characterization</h2> | ||
</html> | </html> | ||
Revision as of 02:11, 4 October 2012
Protocols
General
Plate ProductionCloning
Removing BioBricks from the RegistryProduction of Competent Cells
Heat Shock Transformation
DNA Purification
Restriction Enzyme Digest
Ligation
PCR (Phusion)
Colony PCR
Characterization
Transmission Electron Microscopy ProtocolFimE Asymmetrical Digest Assay Protocol
