Team:Michigan/Notebook/Protocols

From 2012.igem.org

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Protocols
Protocols
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<h2>General</h2>
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Plate Production
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<h2>Cloning</h2>
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!Protocols
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Removing BioBricks from the Registry Wells[[File:MichRem.pdf]]
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; Heat Shock
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<!-- This page will be updated to display previous versions in a dropdown box (HTML select embedded in a form) soon. In the meantime, upload protocols, or link to previous wiki or external websites.-->
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Production of Competent Cells
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[[File:Quick heat shock protocol v1.0.pdf]]
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[[File:Heat shock protocol with SOC rescue v1.1.pdf]]
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Heat Shock Transformation
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[[File:Heat shock protocol with SOC rescue v1.0.pdf]]
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DNA Purification
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;Gel electrophoresis
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[[File:Gel electrophoresis v1.0.pdf]]
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Restriction Enzyme Digest
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Ligation
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PCR (Phusion)
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Colony PCR
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<h2>Characterization</h2>
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Transmission Electron Microscopy Protocol
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FimE Asymmetrical Digest Assay Protocol
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Revision as of 02:05, 4 October 2012

Xayona Website Template

Protocols

General

Plate Production

Cloning

Removing BioBricks from the Registry WellsFile:MichRem.pdf

Production of Competent Cells

Heat Shock Transformation

DNA Purification

Restriction Enzyme Digest

Ligation

PCR (Phusion)

Colony PCR

Characterization

Transmission Electron Microscopy Protocol

FimE Asymmetrical Digest Assay Protocol

Retrieved from "http://2012.igem.org/Team:Michigan/Notebook/Protocols"