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Revision as of 12:24, 24 September 2012 (view source) Ehoyer (Talk | contribs) ← Older edit		Latest revision as of 03:54, 27 September 2012 (view source) HSpedding (Talk | contribs)
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	<center>Centrifuged the preparations for 3 mins @ 13,000 rpm.</center> <br>		<center>Centrifuged the preparations for 3 mins @ 13,000 rpm.</center> <br>
	<center>Loaded 20 uL of the supernatant in to the gel. </center> <br>		<center>Loaded 20 uL of the supernatant in to the gel. </center> <br>
		+	<center>Electrophoresis was conducted at constant voltage (200 V) for one hour. </center> <br>

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Latest revision as of 03:54, 27 September 2012

SDS-PAGE

Pelleted cells were resuspended in 200 uL Milli-Q H2O.
Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer.
Using a Hamilton syringe, the cells were sheared.
Centrifuged the preparations for 3 mins @ 13,000 rpm.
Loaded 20 uL of the supernatant in to the gel.
Electrophoresis was conducted at constant voltage (200 V) for one hour.