Team:Ehime-Japan
From 2012.igem.org
(16 intermediate revisions not shown) | |||
Line 123: | Line 123: | ||
<br> | <br> | ||
- | |||
- | |||
- | |||
- | |||
</p> | </p> | ||
- | <img src="https:// | + | <img src="https://static.igem.org/mediawiki/2012/7/73/%E6%96%B0%EF%BD%82%E5%9B%9B%E3%82%B3%E3%83%9E.jpg" |
- | width=" | + | width="800px"> |
Line 136: | Line 132: | ||
<p> | <p> | ||
<font size="6"> | <font size="6"> | ||
+ | <br> | ||
+ | <br><img src="https://static.igem.org/mediawiki/2012/e/eb/Home%E3%81%AE%EF%BC%91.png" width=800px> | ||
- | + | ||
<br> | <br> | ||
<br> | <br> | ||
<br> | <br> | ||
- | |||
</p> | </p> | ||
<p> | <p> | ||
Line 147: | Line 144: | ||
<br> | <br> | ||
<font size="5"> | <font size="5"> | ||
- | + | <img src=https://static.igem.org/mediawiki/2012/0/0d/Our_project.png width=800px> | |
- | < | + | |
<font size="3"> | <font size="3"> | ||
<p> | <p> | ||
Line 154: | Line 150: | ||
To realize our project, we used the light sensor genes from <i>Synechocystis</i>, the Lon protease and the <i>ssrA</i> tag from <i>Mesoplasma florum</i>. Also, a cell-cell signaling system and the heat shock and cold shock promoters were used so as to construct a foundational system of E.colingual. | To realize our project, we used the light sensor genes from <i>Synechocystis</i>, the Lon protease and the <i>ssrA</i> tag from <i>Mesoplasma florum</i>. Also, a cell-cell signaling system and the heat shock and cold shock promoters were used so as to construct a foundational system of E.colingual. | ||
</p> | </p> | ||
+ | <br> | ||
+ | <br> | ||
<br> | <br> | ||
<font size="5"> | <font size="5"> | ||
- | + | ||
+ | <img src="https://static.igem.org/mediawiki/2012/3/3d/Ecomail.png" width=800px> | ||
</font> | </font> | ||
<br> | <br> | ||
<br> | <br> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/c/c8/E.comail.png" width=700px> | ||
+ | <br><br> | ||
+ | |||
+ | |||
+ | |||
+ | |||
<font size="3"> | <font size="3"> | ||
<p> | <p> | ||
Line 166: | Line 171: | ||
</p> | </p> | ||
<br> | <br> | ||
- | See more… | + | <a href="https://2012.igem.org/Team:Ehime-Japan/Project#one"><p><font color=blue>See more…</font color><p></a> |
<br> | <br> | ||
<br> | <br> | ||
<br> | <br> | ||
<font size="5"> | <font size="5"> | ||
- | + | <img src="https://static.igem.org/mediawiki/2012/2/2d/Ecodomino_ehime_un.png" width=800px> | |
<br> | <br> | ||
<br> | <br> | ||
<font size="3"> | <font size="3"> | ||
- | <p> | + | <p><br><br> |
+ | <img src="https://static.igem.org/mediawiki/2012/9/9d/Eco-Domino.png" width=700px><br><br> | ||
We tried to reconstruct toppling dominoes with <i>E. coli</i>, | We tried to reconstruct toppling dominoes with <i>E. coli</i>, | ||
- | and we would be able to draw a movie of firework on LB plate! | + | and we would be able to draw a movie of firework on LB plate! |
+ | <br><img src="https://static.igem.org/mediawiki/2012/f/f4/%E2%91%A6.png"width="200px"> | ||
+ | |||
</p> | </p> | ||
<br> | <br> | ||
Line 185: | Line 193: | ||
<br> | <br> | ||
<font size="5"> | <font size="5"> | ||
- | + | <img src="https://static.igem.org/mediawiki/2012/a/a2/Sensing.png" width=800px><br> | |
- | <br> | + | |
<br> | <br> | ||
<font size="3"> | <font size="3"> | ||
Line 198: | Line 205: | ||
</p> | </p> | ||
<br> | <br> | ||
- | See more… | + | <a href="https://2012.igem.org/Team:Ehime-Japan/Project#three"><p><font color=blue>See more…</font color><p></a> |
<BR> | <BR> | ||
<BR> | <BR> | ||
Line 204: | Line 211: | ||
<font size="5"> | <font size="5"> | ||
- | + | <img src="https://static.igem.org/mediawiki/2012/0/01/Ecolingual.png" width=800px> | |
<br> | <br> | ||
<br> | <br> | ||
<font size="3"> | <font size="3"> | ||
- | <p> | + | <p><br><br> |
+ | <img src=" | ||
+ | https://static.igem.org/mediawiki/2012/9/93/E.colingual.png" width=800px><br><br> | ||
It consists of three parts:Main, Connection, and Screen. E.colingual is a tool through which | It consists of three parts:Main, Connection, and Screen. E.colingual is a tool through which | ||
we can know <i>E. coli</i> feelings:“hot”,“cold”, “happy” ,or “dying” etc… | we can know <i>E. coli</i> feelings:“hot”,“cold”, “happy” ,or “dying” etc… | ||
Line 216: | Line 225: | ||
</p> | </p> | ||
<br> | <br> | ||
- | See | + | <a href=""><p><font color=blue>See more…</font color><p></a> |
<br> | <br> | ||
<br> | <br> | ||
<br> | <br> | ||
<hr> | <hr> | ||
+ | <br> | ||
+ | <br> | ||
<font size="5"> | <font size="5"> | ||
→ New degradation system | → New degradation system | ||
Line 233: | Line 244: | ||
</p> | </p> | ||
<br> | <br> | ||
- | See more… | + | <a href="https://2012.igem.org/Team:Ehime-Japan/Project#one"><p><font color=blue>See more…</font color><p></a> |
<br> | <br> | ||
<br> | <br> | ||
<br><hr> | <br><hr> | ||
+ | <br> | ||
+ | <br> | ||
<font size="5"> | <font size="5"> | ||
→ New parts | → New parts | ||
Line 249: | Line 262: | ||
</p> | </p> | ||
<br> | <br> | ||
- | See more… | + | <a href="https://2012.igem.org/Team:Ehime-Japan/Parts"><p><font color=blue>See more…</font color><p></a> |
<br> | <br> | ||
<br> | <br> | ||
<br><hr> | <br><hr> | ||
+ | <br> | ||
+ | <br> | ||
<font size="5"> | <font size="5"> | ||
→ Human Practice | → Human Practice | ||
Line 267: | Line 282: | ||
・Ecreate ! | ・Ecreate ! | ||
+ | <br> | ||
<br> | <br> | ||
<br> | <br> | ||
Line 277: | Line 293: | ||
</p> | </p> | ||
<br> | <br> | ||
- | See more… | + | <a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-E.create"><p><font color=blue>See more…</font color><p></a> |
<br> | <br> | ||
- | + | <font size="5"> | |
- | <font size="5"> | + | |
- | + | ||
・Questionnaire | ・Questionnaire | ||
<br> | <br> | ||
Line 292: | Line 306: | ||
</p> | </p> | ||
<br> | <br> | ||
- | See more… | + | <a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-Questionnaire"><p><font color=blue>See more…</font color><p></a> |
<br> | <br> | ||
<HR size="10" width="100%" align="left"> | <HR size="10" width="100%" align="left"> |
Latest revision as of 03:52, 27 September 2012
![](https://static.igem.org/mediawiki/2012/7/73/%E6%96%B0%EF%BD%82%E5%9B%9B%E3%82%B3%E3%83%9E.jpg)
Ehime-Japan team challenged to three projects, which would finally lead us to create “E.colingual”, a tool through which we can know E. coli feelings. It consists of three parts: Main, Connection, and Screen.
“E.co-mail” is a communication tool with E. coli and an optical fiber. We would be able to transfer digital information depicted in E.coli through a distance, and probably could send e-mails with this tool.
See more… See more…
Main is comprised of two different kinds of E. coli transformants.
One is that feels heat and cold and respond differently, producing different
signaling molecules. The other catches the signaling molecules and convert
the signal into the form of light. As a result, we can see a temperature change
around the E. coli. The light signals could be transferred through a distance
and be displayed if the Connection and Screen systems work.
See more… See more…
We used two different ssrA tags to make a circulatable communication system. One is from
M. florum recognized by the Lon protease from M. florum. The other is E. coli ssrA tag (LVA).
Because the sequences of those tags are not similar, the degradation systems should function
independently.
See more…
We made new BioBrick parts!
See more…
Ehime-Japan did a lot of projects as Human Practice!
We are enjoying gene recombination, and to tell interests of synthetic biology for
high school students, we made “E.create”!! It is a card game that people can enjoy
building genetic circuits. Also, we did educational activities for high school students
and played them with E.create!
See more…
Our survey was carried out by Ehime-Japan, Osaka and KIT-Kyoto iGEM 2012 teams together!
Thus, we were able to obtain a lot of replies, and found more general opinions
about synthetic biology in Japan.
See more…
To realize our project, we used the light sensor genes from Synechocystis, the Lon protease and the ssrA tag from Mesoplasma florum. Also, a cell-cell signaling system and the heat shock and cold shock promoters were used so as to construct a foundational system of E.colingual.
We tried to reconstruct toppling dominoes with E. coli,
and we would be able to draw a movie of firework on LB plate!
It consists of three parts:Main, Connection, and Screen. E.colingual is a tool through which
we can know E. coli feelings:“hot”,“cold”, “happy” ,or “dying” etc…
Also, by an optical fiber, the information of those feelings is transmitted
to someone in a distant place from a lab. Actually, we focused on E. coli
feelings, “hot” and “cold”.
→ New degradation system
→ New parts
・BBa_K794000 : This part is cold shock promoter + lasI.
→ Human Practice
・Ecreate !
・Questionnaire
Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
---|