Team:Ehime-Japan
From 2012.igem.org
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+ | <ul id="nav"> | ||
+ | <li><a href="https://2012.igem.org/Team:Ehime-Japan">Home</a></li> | ||
+ | <li><a href="https://2012.igem.org/Team:Ehime-Japan/Project">Project</a> | ||
+ | <ul> | ||
+ | <li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#one">E.co-mail</a></li> | ||
+ | <li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#two">E.co-Domino</a></li> | ||
+ | <li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#three">E.cold-heat sensing system</a></li> | ||
+ | <li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#four">E.colingual</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2012.igem.org/Team:Ehime-Japan/Modeling">Modeling</a></li> | ||
+ | <li><a href="#">Data</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2012.igem.org/Team:Ehime-Japan/Parts">BioBrick parts</a></li> | ||
+ | <li><a href="https://2012.igem.org/Team:Ehime-Japan/PlasmidsAndStrain">Plasmids And Strain</a></li> | ||
+ | <li><a href="https://2012.igem.org/Team:Ehime-Japan/PrimerList">Primer List</a></li> | ||
+ | |||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="#">Human Practice</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-E.create">E.create</a></li> | ||
+ | <li><a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-Questionnaire">Questionnaire</a></li> | ||
+ | |||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="#">Other</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2012.igem.org/Team:Ehime-Japan/Team">team</a></li> | ||
+ | <li><a href="https://igem.org/Team.cgi?year=2012&team_name=Ehime-Japan">team profile</a></li> | ||
+ | <li><a href="https://2012.igem.org/Team:Ehime-Japan/Notebook">note book</a></li> | ||
+ | <li><a href="https://2012.igem.org/Team:Ehime-Japan/Safety">safety</a></li> | ||
+ | <li><a href="https://2012.igem.org/Team:Ehime-Japan/Attributions">attribution</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
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- | < | + | <br> |
- | < | + | </p> |
+ | <img src="https://static.igem.org/mediawiki/2012/7/73/%E6%96%B0%EF%BD%82%E5%9B%9B%E3%82%B3%E3%83%9E.jpg" | ||
+ | width="800px"> | ||
- | < | + | <HR size="10" width="100%" align="left"> |
+ | <p> | ||
+ | <font size="6"> | ||
+ | <br> | ||
+ | <br><img src="https://static.igem.org/mediawiki/2012/e/eb/Home%E3%81%AE%EF%BC%91.png" width=800px> | ||
+ | |||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | </p> | ||
+ | <p> | ||
- | < | + | <br> |
+ | <font size="5"> | ||
+ | <img src=https://static.igem.org/mediawiki/2012/0/0d/Our_project.png width=800px> | ||
+ | <font size="3"> | ||
+ | <p> | ||
+ | Ehime-Japan team challenged to three projects, which would finally lead us to create “E.colingual”, a tool through which we can know <i>E. coli</i> feelings. It consists of three parts: Main, Connection, and Screen.<br> | ||
+ | To realize our project, we used the light sensor genes from <i>Synechocystis</i>, the Lon protease and the <i>ssrA</i> tag from <i>Mesoplasma florum</i>. Also, a cell-cell signaling system and the heat shock and cold shock promoters were used so as to construct a foundational system of E.colingual. | ||
+ | </p> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <font size="5"> | ||
- | < | + | <img src="https://static.igem.org/mediawiki/2012/3/3d/Ecomail.png" width=800px> |
- | + | </font> | |
- | + | <br> | |
- | < | + | <br> |
- | + | <img src="https://static.igem.org/mediawiki/2012/c/c8/E.comail.png" width=700px> | |
- | + | <br><br> | |
- | < | + | |
- | + | ||
- | + | ||
- | < | + | |
+ | <font size="3"> | ||
+ | <p> | ||
+ | “E.co-mail” is a communication tool with <i>E. coli</i> and an optical fiber. We would be able to transfer digital information depicted in <i>E.coli</i> through a distance, and probably could send e-mails with this tool. | ||
+ | </p> | ||
<br> | <br> | ||
- | + | <a href="https://2012.igem.org/Team:Ehime-Japan/Project#one"><p><font color=blue>See more…</font color><p></a> | |
<br> | <br> | ||
- | |||
<br> | <br> | ||
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<br> | <br> | ||
- | + | <font size="5"> | |
- | <img src="https://static.igem.org/mediawiki/2012/ | + | <img src="https://static.igem.org/mediawiki/2012/2/2d/Ecodomino_ehime_un.png" width=800px> |
<br> | <br> | ||
- | |||
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+ | <font size="3"> | ||
+ | <p><br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/9/9d/Eco-Domino.png" width=700px><br><br> | ||
+ | We tried to reconstruct toppling dominoes with <i>E. coli</i>, | ||
+ | and we would be able to draw a movie of firework on LB plate! | ||
+ | <br><img src="https://static.igem.org/mediawiki/2012/f/f4/%E2%91%A6.png"width="200px"> | ||
+ | </p> | ||
<br> | <br> | ||
- | + | <a href="https://2012.igem.org/Team:Ehime-Japan/Project#two"><p><font color=blue>See more…</font color><p></a> | |
<br> | <br> | ||
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<br> | <br> | ||
- | + | <br> | |
- | + | <font size="5"> | |
- | + | <img src="https://static.igem.org/mediawiki/2012/a/a2/Sensing.png" width=800px><br> | |
- | + | <br> | |
- | + | <font size="3"> | |
- | + | <p> | |
- | + | Main is comprised of two different kinds of <i>E. coli</i> transformants. | |
- | + | One is that feels heat and cold and respond differently, producing different | |
- | <font size="5" | + | signaling molecules. The other catches the signaling molecules and convert |
- | < | + | the signal into the form of light. As a result, we can see a temperature change |
- | < | + | around the <i>E. coli</i>. The light signals could be transferred through a distance |
- | + | and be displayed if the Connection and Screen systems work. | |
- | < | + | </p> |
+ | <br> | ||
+ | <a href="https://2012.igem.org/Team:Ehime-Japan/Project#three"><p><font color=blue>See more…</font color><p></a> | ||
<BR> | <BR> | ||
<BR> | <BR> | ||
<BR> | <BR> | ||
- | <font size="5"> | + | <font size="5"> |
+ | <img src="https://static.igem.org/mediawiki/2012/0/01/Ecolingual.png" width=800px> | ||
+ | <br> | ||
+ | <br> | ||
+ | <font size="3"> | ||
+ | <p><br><br> | ||
+ | <img src=" | ||
+ | https://static.igem.org/mediawiki/2012/9/93/E.colingual.png" width=800px><br><br> | ||
+ | It consists of three parts:Main, Connection, and Screen. E.colingual is a tool through which | ||
+ | we can know <i>E. coli</i> feelings:“hot”,“cold”, “happy” ,or “dying” etc… | ||
+ | Also, by an optical fiber, the information of those feelings is transmitted | ||
+ | to someone in a distant place from a lab. Actually, we focused on <i>E. coli</i> | ||
+ | feelings, “hot” and “cold”. | ||
+ | </p> | ||
+ | <br> | ||
+ | <a href=""><p><font color=blue>See more…</font color><p></a> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <hr> | ||
+ | <br> | ||
+ | <br> | ||
+ | <font size="5"> | ||
+ | → New degradation system | ||
+ | <br> | ||
+ | <br> | ||
+ | <font size="3"> | ||
+ | <p> | ||
+ | We used two different <i>ssrA</i> tags to make a circulatable communication system. One is from | ||
+ | <i>M. florum</i> recognized by the Lon protease from <i>M. florum</i>. The other is <i>E. coli ssrA</i> tag (LVA). | ||
+ | Because the sequences of those tags are not similar, the degradation systems should function | ||
+ | independently. | ||
+ | </p> | ||
+ | <br> | ||
+ | <a href="https://2012.igem.org/Team:Ehime-Japan/Project#one"><p><font color=blue>See more…</font color><p></a> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br><hr> | ||
+ | <br> | ||
+ | <br> | ||
+ | <font size="5"> | ||
+ | → New parts | ||
+ | <br> | ||
+ | <br> | ||
+ | <font size="3"> | ||
+ | <p> | ||
+ | |||
+ | We made new BioBrick parts! | ||
+ | <br> | ||
+ | ・BBa_K794000 : This part is cold shock promoter + <i>lasI</i>. | ||
+ | </p> | ||
+ | <br> | ||
+ | <a href="https://2012.igem.org/Team:Ehime-Japan/Parts"><p><font color=blue>See more…</font color><p></a> | ||
+ | |||
+ | <br> | ||
+ | <br> | ||
+ | <br><hr> | ||
+ | <br> | ||
+ | <br> | ||
+ | <font size="5"> | ||
+ | → Human Practice | ||
+ | <br> | ||
+ | <br> | ||
+ | <font size="3"> | ||
+ | <p> | ||
+ | Ehime-Japan did a lot of projects as Human Practice! | ||
+ | </p> | ||
+ | <br> | ||
+ | <br> | ||
+ | <font size="5"> | ||
+ | |||
+ | ・Ecreate ! | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <font size="3"> | ||
+ | <p> | ||
+ | We are enjoying gene recombination, and to tell interests of synthetic biology for | ||
+ | high school students, we made “E.create”!! It is a card game that people can enjoy | ||
+ | building genetic circuits. Also, we did educational activities for high school students | ||
+ | and played them with E.create! | ||
+ | </p> | ||
+ | <br> | ||
+ | <a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-E.create"><p><font color=blue>See more…</font color><p></a> | ||
+ | <br> | ||
+ | <font size="5"> | ||
+ | ・Questionnaire | ||
+ | <br> | ||
+ | <br> | ||
+ | <font size="3"> | ||
+ | <p> | ||
+ | Our survey was carried out by Ehime-Japan, Osaka and KIT-Kyoto iGEM 2012 teams together! | ||
+ | Thus, we were able to obtain a lot of replies, and found more general opinions | ||
+ | about synthetic biology in Japan. | ||
+ | </p> | ||
+ | <br> | ||
+ | <a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-Questionnaire"><p><font color=blue>See more…</font color><p></a> | ||
+ | <br> | ||
<HR size="10" width="100%" align="left"> | <HR size="10" width="100%" align="left"> | ||
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Latest revision as of 03:52, 27 September 2012
![](https://static.igem.org/mediawiki/2012/7/73/%E6%96%B0%EF%BD%82%E5%9B%9B%E3%82%B3%E3%83%9E.jpg)
Ehime-Japan team challenged to three projects, which would finally lead us to create “E.colingual”, a tool through which we can know E. coli feelings. It consists of three parts: Main, Connection, and Screen.
“E.co-mail” is a communication tool with E. coli and an optical fiber. We would be able to transfer digital information depicted in E.coli through a distance, and probably could send e-mails with this tool.
See more… See more…
Main is comprised of two different kinds of E. coli transformants.
One is that feels heat and cold and respond differently, producing different
signaling molecules. The other catches the signaling molecules and convert
the signal into the form of light. As a result, we can see a temperature change
around the E. coli. The light signals could be transferred through a distance
and be displayed if the Connection and Screen systems work.
See more… See more…
We used two different ssrA tags to make a circulatable communication system. One is from
M. florum recognized by the Lon protease from M. florum. The other is E. coli ssrA tag (LVA).
Because the sequences of those tags are not similar, the degradation systems should function
independently.
See more…
We made new BioBrick parts!
See more…
Ehime-Japan did a lot of projects as Human Practice!
We are enjoying gene recombination, and to tell interests of synthetic biology for
high school students, we made “E.create”!! It is a card game that people can enjoy
building genetic circuits. Also, we did educational activities for high school students
and played them with E.create!
See more…
Our survey was carried out by Ehime-Japan, Osaka and KIT-Kyoto iGEM 2012 teams together!
Thus, we were able to obtain a lot of replies, and found more general opinions
about synthetic biology in Japan.
See more…
To realize our project, we used the light sensor genes from Synechocystis, the Lon protease and the ssrA tag from Mesoplasma florum. Also, a cell-cell signaling system and the heat shock and cold shock promoters were used so as to construct a foundational system of E.colingual.
We tried to reconstruct toppling dominoes with E. coli,
and we would be able to draw a movie of firework on LB plate!
It consists of three parts:Main, Connection, and Screen. E.colingual is a tool through which
we can know E. coli feelings:“hot”,“cold”, “happy” ,or “dying” etc…
Also, by an optical fiber, the information of those feelings is transmitted
to someone in a distant place from a lab. Actually, we focused on E. coli
feelings, “hot” and “cold”.
→ New degradation system
→ New parts
・BBa_K794000 : This part is cold shock promoter + lasI.
→ Human Practice
・Ecreate !
・Questionnaire
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