Team:BAU-Indonesia/Attributions

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<h2><b>Safety</b></h2>
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<h2><b>Attributions</b></h2>
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<br> <div class="text">
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  <h3>
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    <strong>1. Would any of your project ideas raise safety issues in
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                                    <img style="border:double" src="https://static.igem.org/mediawiki/2012/e/e0/Attribution.png"/><br><br><br>
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    <h4 style="text-align:justify">
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    terms of:
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                                      The project is carried out in stages starting from sampling crumbs plastic in landfills Galuga on the 2nd of June 2012, then isolated on agar media. Transferred from a liquid medium is expected there will be a single cell that contains genes kutinase using enrichment medium. Continued to perform the isolation of DNA from the sample by extraction with host lab is divided by the division of labor among:<br><br>
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                                        </strong>
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1. Modelling Biobrick : Making construction of BioBrick design<br>
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  </h3><br>                                    
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2. Isolasi    : Making growth media, perform the isolation of the samples will be given further treatment<br>
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  <h4><strong>Researcher Safety</strong><br></h4>
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3. Ligation and Transformation : Doing stage followed by ligation of the sample on the sample stage of transformation<br>
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    <h4>We have isolated and cultured the bacteria sample from landfill
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4. Notebook : Document any activities that have been done so visible track record of activity in the form of notes and drawings<br>
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5. Degradation Test  : Test results obtained are expected to contain genes that are qualitatively and quantitatively<br>
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    at Galuga, West Java Indonesia. To prevent contamination by some
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6. Project Report and Literature: look at the literature related to the project that we are doing to see comparison and as reference material) and report the results of the project<br>
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7. Project leader Wetlab :Directing the activities wetlab<br>
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    harmfull bacteria, we have followed the instruction of safety
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8. Advisors : Provide guidance related to what we have to carry out in particular in terms of the molecular laboratory work<br>
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9. Instructors: Provide technical workmanship molecular laboratory activities every activity we will do<br>
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    procedure, such as using sensigloves, mask, and washing hand
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10. Technicians : Provide technical assistance in the laboratory work on all aspects of molecular construction and provision of laboratory materials<br>
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11. Professional Website Designer : Website design director<br>
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    after sampling. Hazardous chemicals that we used are aclrilamide
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12. Artist    : perform work related to the project such as design mascot, poster<br>
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13. Negotiator leader : a team representative in negotiating cooperation with external parties<br>
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    and ethidium bromyde. Acrylamide (or acrylic amide) is a simple
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14. Budgetting and accommodation: designing financial needs during the activity<br>
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15.Fundrising team: team looking for fundraising<br>
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    organic compound with the chemical formula C3H5NO. It's
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16. Project report, authorship, acknowledgement of source and references:  Reported overall activity and gives an idea of ​​how different the research we do<br>
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17. Biobrick modeling: assemble a system to perform bio synthetic
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    potentially hazardous to health (cancer or carcinogenic).
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18. Poster: create a poster to illustrate the activities we have done with the results of the activity as a whole<br><br>
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    Acrylamide can form long polymer chains known as polyacrylamide,
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Special Thanks to:<br>
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1. Dr.Ir. Ence Darmo Jaya Supena, MS<br>
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    which is also carcinogenic. Inhalation: irritation,
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2. Dr. Ir. Rimbawan<br>
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3. Dr. Ir. Sri Nurdiati, M.Sc<br>
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    disorientation, burning, convulsions. Contact with skin:
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3. Dr. Ir. Agus Buono, M.Si M.Kom<br>
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4. Prof.Dr.Ir. Antonius Suwanto<br>
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    irritation, disorientation. Eye contact: irritation, a burning
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5. Dr. Ir. Sulistijorini, M.Si<br>
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6. Dr. Dra. Anja Meryandini, MS<br>
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    sensation of the eyes, eye damage.
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7. Dr. Dra. Nisa Rachmania, M.Si<br>
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    Ethidium bromide will reduce the rate of migration of linear DNA  
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8. Esa Ayu Pratama<br>
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9. Bulan<br>
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    molecules by 15%. This solution is extremely dangerous and
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10. Liliani Isna Devi    <br>                                
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11. Prof. Dr.Suminar Setiati Achmadi <br>
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    carcinogenic. All solutions which contained ethidium bromide
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12. Miss Tetty Kemala<br>        
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13. Dr. Iman Rusmana, M.si<br>                          
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    should be decontaminated before disposal. Ethidium Bromide
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</h4><br>    
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    (EtBr) will fluoresce under UV exposure. These dyes can be added
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    to the gel and buffer so there is no staining after
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    electrophoresis process. The advantage is more practical, but
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    the possibility of EtBr contamination is greater. Due to the
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    carcinogenic factor, it must be avoided for direct contact.<br> </h4>
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    <h4><strong>Public Safety</strong><br></h4>
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    <h4>We have ethics to minimize the danger possibility to public. We
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    usually wear single-used gloves, covered shoes, lab coat, and
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    mask so the accumulation of materials and impurities are not
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    exposed to participant directly. We have a liquid discharge
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    valves which equipped with running water, so it won't settle. We
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    also have a separated solid waste disposal for potentially
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    hazardous materials. Those ethics are rules in our work on this
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    project. </h4>
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                                        <br>  
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    <h4><strong>Environmental Safety</strong><br></h4>
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  <h4> No harmful effect on our enzyme product. E. coli bacteria which
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    inserted by Cutinase gene has been set for its life period by
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    sonicator so there'll no blooming act in nature. Destruction of  
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    PET by using our products is done at indoor laboratory so the  
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    waste does not impact to the environment directly. The enzyme
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    does not cause further effect especially for chemical / physical
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    danger.No harmful effect on our enzyme product. E. coli bacteria
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    which inserted by  Cutinase gene has been set for its life
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    period by sonicator so there'll no blooming act in nature.
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    Destruction of PET by using our products is done at indoor
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    laboratory so the waste does not impact to the environment
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    directly. The enzyme does not cause further effect especially
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      for chemical / physical danger.
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  </h4>                                      <br>
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  <h3> <strong>2. Do any of the new BioBrick parts (or devices) that you
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    made this year raise safety issues? If yes, Did you document these issues in the Registry?</strong> </h3><br>
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    <h4>No, we didn't document the safety issue to the registry, but our
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    procedure was according the Standard Operational Procedure of  
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    Molecular Biology Laboratory and Microbiology laboratory in IPB
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    (Bogor Agricultural University). So we think our project hasn't
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    any safety risk during we follow the procedure carefully.</h4><br>  
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    <h4><strong>How did you manage to handle the safety issue?</strong></h4><br>
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  <h4> To handle the security issue of our work, we always consult with
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    the team advisor and instructor who handle the laboratory
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    security work in IPB. Our security is guaranteed by following
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    the standard operating procedures which provided in each
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    laboratory. As a mechanism to ensure the safety of our work,
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    first of all we started it by product testing step in the
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    laboratory before applying it in nature, so when the result is
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    guaranteed safely, it can be applied in nature.</h4><br>  
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    <h4><strong>How could other teams learn from your experience?</strong></h4><br>
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  <h4> Other teams can learn from the procedure that we have used. We
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    use the common procedure that is used by molecular researchers
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    at the laboratory, so its safety has been tested (as long as we
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      follow the standard operation procedure had been given).                                    
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      </h4><br>
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    <h3><strong>3. Is there a local biosafety group, committee, or review
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    board at your institution? </strong><h3> <br>
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      <h4><strong>Which specific biosafety rules or guidelines do you have to
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    consider in your country?</strong></h4><br>
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  <h4> We have no local biosafety committee at our institution but
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    there are Biosafety rules or guidelines in our country. Please
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    see below :<br>
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    1. Regulation Law No. 32 of 2009 about Environmental
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    Protection & Management<br>  
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    2. Law No. 7 of 1996 on Food<br>
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    3. Law No. 18 of 2002 on National System
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    Research,Development and Application of Science and
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    Technology<br>
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    4. Law No. 18 of 2004 on Plantation<br>
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    5. Law No. 23 of 1992 on Health<br>
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    6. Law No. 23 of 1992 on Animal, Fish, and Plant
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    Quarantine<br>
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    7. Law No. 41 of 1999 on Forestry<br>
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    8. Government Regulation No. 21 of 2005 on Biosafety of
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    Genetically Engineered Product<br>
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    9. Presidential Decree 39 of 2010 on Biosafety Commision of
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    Genetically Engineered Product.<br>
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    10. INSTITUTIONAL ARRANGEMENT Biosafety Commision
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    Established by Presidential Decree No. 39/2010 <br>
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    11. Biosafety Technical Team In the establishment
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    process.<br>
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    12. Biosafety Clearing House Regarding Article 20 of the
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    Cartagena Protocol, Indonesia has been establish a Biosafety
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    Clearing House to facilitate the exchange of scientific,
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    technical , environmental and legalinformation on LMO. BCH is
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    coordinated by Ministry of Environment in cooperation with
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    Indonesia Institute of Science Secretariat of Biosafety
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    Commission.<br>
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    13. Law 32/2009. Every activities may create important risk
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    on environment, biodiversity and human healthand human safety
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    have to carry out Environmental Risk Analysis, Risk Assessment,
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      Risk Management, Risk Communication.                                                                                                                  
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      </h4><br>
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        <h3><strong>4. Do you have any other ideas how to deal with safety
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        issues that could be useful for future iGEM competitions? How
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        could parts, devices and systems be made even safer through
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          biosafety engineering? </strong>
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      </h3>                                      <br>
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      <h4>To ensure the biosafety, it will be better to give more
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        certification letter about the safety of materials which
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        provided to the participants. Each institution must have their
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        own fire fighters and laboratory clinic centre.
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        To minimize the hazardous chemicals we can use the synthetic
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        biology engineered system. The equipment which is used is fairly
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        easy to control for these biosafety ethics. The example of
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        recommended system that has better security is JSA (Job Safety
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        Analyst).</h4>
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Latest revision as of 03:52, 27 September 2012


Metamorphosis Design Free Css Templates

Attributions





The project is carried out in stages starting from sampling crumbs plastic in landfills Galuga on the 2nd of June 2012, then isolated on agar media. Transferred from a liquid medium is expected there will be a single cell that contains genes kutinase using enrichment medium. Continued to perform the isolation of DNA from the sample by extraction with host lab is divided by the division of labor among:

1. Modelling Biobrick : Making construction of BioBrick design
2. Isolasi : Making growth media, perform the isolation of the samples will be given further treatment
3. Ligation and Transformation : Doing stage followed by ligation of the sample on the sample stage of transformation
4. Notebook : Document any activities that have been done so visible track record of activity in the form of notes and drawings
5. Degradation Test : Test results obtained are expected to contain genes that are qualitatively and quantitatively
6. Project Report and Literature: look at the literature related to the project that we are doing to see comparison and as reference material) and report the results of the project
7. Project leader Wetlab :Directing the activities wetlab
8. Advisors : Provide guidance related to what we have to carry out in particular in terms of the molecular laboratory work
9. Instructors: Provide technical workmanship molecular laboratory activities every activity we will do
10. Technicians : Provide technical assistance in the laboratory work on all aspects of molecular construction and provision of laboratory materials
11. Professional Website Designer : Website design director
12. Artist : perform work related to the project such as design mascot, poster
13. Negotiator leader : a team representative in negotiating cooperation with external parties
14. Budgetting and accommodation: designing financial needs during the activity
15.Fundrising team: team looking for fundraising
16. Project report, authorship, acknowledgement of source and references: Reported overall activity and gives an idea of ​​how different the research we do
17. Biobrick modeling: assemble a system to perform bio synthetic 18. Poster: create a poster to illustrate the activities we have done with the results of the activity as a whole

Special Thanks to:
1. Dr.Ir. Ence Darmo Jaya Supena, MS
2. Dr. Ir. Rimbawan
3. Dr. Ir. Sri Nurdiati, M.Sc
3. Dr. Ir. Agus Buono, M.Si M.Kom
4. Prof.Dr.Ir. Antonius Suwanto
5. Dr. Ir. Sulistijorini, M.Si
6. Dr. Dra. Anja Meryandini, MS
7. Dr. Dra. Nisa Rachmania, M.Si
8. Esa Ayu Pratama
9. Bulan
10. Liliani Isna Devi
11. Prof. Dr.Suminar Setiati Achmadi
12. Miss Tetty Kemala
13. Dr. Iman Rusmana, M.si


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