Team:SUSTC-Shenzhen-B/safety

Safety

The lab part of our project is basic molecule biology and cell biology experiment. There are not many problems in safety. The common sides in biological lab safety are biological hazard, chemical damage and physical danger.

Firstly, about biological hazard, the risk doesn’t exist in our lab mainly. The genes we need are GFP, RFP and AMP. They are harmless no matter when transcription and translation. Besides, the E. coli we used has already undergone mutation in gene level, which means the E. coli we used can’t cause any disease.

Secondly, as for chemical damage, there are some processes we have to care. The common method to check up the result of enzyme digestion and link of DNA fragment is electrophoresis. In a important process of electrophoresis, DNA is dyed by ethidium bromide. Ethidium bromide is a kind of poison. It is bad for our people health, and may cause cancer. So we must take care of it.

Finally, in physical danger, the most dangerous being is ultraviolet in the whole lab process, because other bacteria or fungi are forbidden appearing in some lab processes.

Besides, about the key questions, we can show our answers to you.

Would any of your project ideas raise safety issues in terms of researcher safety, public safety, or environmental safety?

Because that we had not done any biological experiments before, and no mentors of experiment came to help us, at the beginning of our lab work, when using ultraviolet rays without protective glasses, we had a little problem with skin.

Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?

our new BioBricks do not raise any foreseeable safety issues. The Biobrick parts we made are transcription terminators, which is not translated into protein.

Is there a local biosafety group, committee, or review board at your institution?

• If yes, what does your local biosafety group think about your project?

• If no, which specific biosafety rules or guidelines do you have to consider in your country?

Many professor and assistant from biology and chemistry department of SUSTC care our safety and have checked the laboratory we used and the processes we have done. And they think the condition we were working is safe and reliable.

Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

We suggest that all the iGEM Teams should have mentors of experiment when doing lab work. We suggest more and more people from different background or major participate in biosafety engineering. Then people can deal with biosafety issues in different way and more completely.