Team:Evry/Freezer
From 2012.igem.org
(Difference between revisions)
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<h1>Freezer</h1> | <h1>Freezer</h1> | ||
- | </ | + | |
+ | |||
+ | |||
+ | <br /> | ||
+ | <center><img src="https://static.igem.org/mediawiki/2012/4/4c/FreezerEVRY.png" width="315px" height="340"></center> | ||
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<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
<td style="background: #ccffcc;">P6 | <td style="background: #ccffcc;">P6 | ||
- | </td><td>Tir1 SdM v02- | + | </td><td>Tir1 SdM v02-Rv |
</td><td>ctagAgcagcattagtcatttgg | </td><td>ctagAgcagcattagtcatttgg | ||
</td><td>23 | </td><td>23 | ||
Line 302: | Line 307: | ||
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
<td style="background: #ccffcc;">P26 | <td style="background: #ccffcc;">P26 | ||
- | </td><td> | + | </td><td>P_colPCR_pSC2+_F |
</td><td>CAAGTGTAGCGGTCACGCTG | </td><td>CAAGTGTAGCGGTCACGCTG | ||
</td><td>20 | </td><td>20 | ||
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</td><td>60 | </td><td>60 | ||
</td><td> | </td><td> | ||
- | </td><td> | + | </td><td> 2nd august |
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
<td style="background: #ccffcc;">P27 | <td style="background: #ccffcc;">P27 | ||
- | </td><td> | + | </td><td>P_colPCR_pSC2+_R |
</td><td>GCGTAATCATGGTCATAGCTG | </td><td>GCGTAATCATGGTCATAGCTG | ||
</td><td>21 | </td><td>21 | ||
Line 318: | Line 323: | ||
</td><td>48 | </td><td>48 | ||
</td><td> | </td><td> | ||
- | </td><td> | + | </td><td> 2nd august |
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
Line 382: | Line 387: | ||
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
<td style="background: #ccffcc;">P34 | <td style="background: #ccffcc;">P34 | ||
- | </td><td> | + | </td><td>IaaH_Kozak_BB_F |
</td><td> GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCATGCGCGAAATGATTACGC | </td><td> GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCATGCGCGAAATGATTACGC | ||
</td><td>60 | </td><td>60 | ||
Line 392: | Line 397: | ||
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
<td style="background: #ccffcc;">P35 | <td style="background: #ccffcc;">P35 | ||
- | </td><td> | + | </td><td>IaaM_Kozak_BB_F |
</td><td> GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCatgtttggaccggatttcc | </td><td> GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCatgtttggaccggatttcc | ||
</td><td>60 | </td><td>60 | ||
Line 399: | Line 404: | ||
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
+ | |||
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
<td style="background: #ccffcc;">P36 | <td style="background: #ccffcc;">P36 | ||
- | </td><td> | + | </td><td>Mut_tyr1_v3_Fw |
- | </td><td> | + | </td><td>CTG CTG TGA CAG AGG AGG GAC TTG TTG CAG TAT CCT TGG GCT GTC CAA AAC TGA ACT CAC TAC TGT ACT TCT GTC ACC AAA TGA CTA ATG CTG CTC TAG TTA CTG TCG CCA AG |
- | </td><td>20 | + | </td><td>113 |
- | </td><td> | + | </td><td>71 |
- | </td><td> | + | </td><td>48 |
- | </td><td> | + | </td><td>Primer to mutate two illegal sites in one time |
- | </td><td> | + | </td><td>08.08 |
+ | |||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P37 </td><td> RFP casette GB - fw </td><td> CCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGAAGACGCTTGAGACCTCTAGACAATACGCAAACCGC </td><td> 71 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P38 </td><td> RFP casette GB - rev </td><td> GGTGACACCTTGCCCTTTTTTGCCGGACTGCAGGAAGACGCTGGAGACCACTAGTTATAAACGCAGAAAGGCCC </td><td> 74 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P39 </td><td> R0010 GB - fw </td><td> CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCACGCTCTAGAGCAATACGCAAACCGCCTCT </td><td> 71 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P40 </td><td> R0010 GB - rev </td><td> GGTGACACCTTGCCCTTTTTTGCCGGACGCTGCAGTACTAGTGGTCTCTGTCTTCTGTGTGAAATTGTTATCCGCTC </td><td> 77 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P41 </td><td> B0034 GB - fw </td><td> CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCTAGACAAAGAGGAGAAATACTAGTGAGACCCTGCAGGTCCGGCAAAAAAGGGCAAGGTGTCACC </td><td> 105 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P42 </td><td> B0034 GB - rev </td><td> GGTGACACCTTGCCCTTTTTTGCCGGACCTGCAGGGTCTCACTAGTATTTCTCCTCTTTGTCTAGAGACCGAATTCCCAGAAATCATCCTTAGCGAAAGCTAAGG </td><td> 105 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P43 </td><td> J61107 GB - fw </td><td> CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCTAGACAAAGAAGAGACTTACTAGTGAGACCCTGCAGGTCCGGCAAAAAAGGGCAAGGTGTCACC </td><td> 105 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P44 </td><td> J61107 GB - rev </td><td> GGTGACACCTTGCCCTTTTTTGCCGGACCTGCAGGGTCTCACTAGTAAGTCTCTTCTTTGTCTAGAGACCGAATTCCCAGAAATCATCCTTAGCGAAAGCTAAGG </td><td> 105 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P45 </td><td> J61117 GB - fw </td><td> CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCTAGACAAAGACATGAGTTACTAGTGAGACCCTGCAGGTCCGGCAAAAAAGGGCAAGGTGTCACC </td><td> 105 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P46 </td><td> J61117 GB - rev </td><td> GGTGACACCTTGCCCTTTTTTGCCGGACCTGCAGGGTCTCACTAGTAACTCATGTCTTTGTCTAGAGACCGAATTCCCAGAAATCATCCTTAGCGAAAGCTAAGG </td><td> 105 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P47 </td><td> E0040 GB - fw </td><td> CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCTCTAGATGCGTAAAGGAGAAGAACTTTTCAC </td><td> 72 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P48 </td><td> E0040 GB - rev </td><td> GGTGACACCTTGCCCTTTTTTGCCGGACCTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGTATAGTTCATCCATGCCATG </td><td> 83 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P49 </td><td> E1010 GB - fw </td><td> CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCTCTAGATGGCTTCCTCCGAAGACG </td><td> 65 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P50 </td><td> E1010 GB - rev </td><td> GGTGACACCTTGCCCTTTTTTGCCGGACCTGCAGACTAGTGGTCTCTGTCTTCTTATTAAGCACCGGTGGAGTG </td><td> 74 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P51 </td><td> B0015 GB - fw </td><td> CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCTAGACCCAGGCATCAAATAAAACG </td><td> 65 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P52 </td><td> B0015 GB - rev </td><td> GGTGACACCTTGCCCTTTTTTGCCGGACCTGCAGGGTCTCCCGCTACTAGTATATAAACGCAGAAAGGCCCAC </td><td> 73 </td><td></td><td></td><td> Golden brick </td><td> 16th August | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P53 </td><td> GFP-AID - fw </td><td> GAATTCGCGGCCGCTTCTAGAGCCACCATGGCCACAACCATGGTG </td><td> 45 </td><td></td><td></td><td> </td><td> 25th August </td> | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P54</td><td>GFP-AID-BBG-fw </td><td> CCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGCCACCATGGTGAGCAAGGGCG </td><td> 72 </td><td></td><td></td><td> </td><td> 25th August </td> | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P55</td><td>GFP-AID-BBG-rev </td><td> GGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATCTGCTCTTGCACTTCTCCATC </td><td> 73 </td><td></td><td></td><td> </td><td> 25th August </td> | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P56</td><td>GFP-AID-2NLS BBG-rev </td><td> GGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTAAACCTTACGTTTCTTTTTAGGGAC </td><td> 75 </td><td></td><td></td><td> </td><td> 25th August </td> | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P57 </td><td> pSC2+ colony primer fw </td><td> CTGGGGACGTCGGAGCAAGC </td><td> 20 </td><td></td><td></td><td> </td><td> 25th August </td> | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P58 </td><td> pSC2+ colony primer rv </td><td> CACTGCATTCTAGTTGTGGTTTGTCCAAACTCATC </td><td> 35 </td><td></td><td></td><td> </td><td> 25th August </td> | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P59</td><td>OsTirI3myc BBG-fw </td><td> CCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCCACCATGACGTACTTCCCGGAGG </td><td> 74 </td><td></td><td></td><td> </td><td> 25th August </td> | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P60</td><td>OsTirI3myc BBG-rev </td><td> GGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTAGTTCAAGTCTTCTTCTGAGATTAATTTTTG </td><td> 84 </td><td></td><td></td><td> </td><td> 25th August </td> | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P61</td><td>GFP-AID-NLS GG -fw </td><td> GCGAATTCGGTCTCACGCTCTAGAGAGCCACCATGGTGAGCAAGGGCG </td><td> 48 </td><td></td><td></td><td> </td><td> 25th August </td> | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P62</td><td>GFP-AID-NLS GG-rev </td><td> CCAGGTCTCACATGTTAGCAGACTTCCTCTGCCCTCTCCACTGCCAACCTTACGTTTCTTTTTAGGGACC </td><td> 70 </td><td></td><td></td><td> </td><td> 25th August </td> | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P63</td><td>OSTirI-GG-fw </td><td> CCTGGTCTCTCATGCGGTGACGTCGAGGAGAATCCTGGCCCAATGACGTACTTCCCGGAGG </td><td> 61 </td><td></td><td></td><td> </td><td> 25th August </td> | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P64</td><td>OSTirI-GG-rev </td><td> GGCTGCAGGGTCTCCCGCTACTAGTATTATTAGTTCAAGTCTTCTTCTGAGATTAATTTTTG </td><td> 62 </td><td></td><td></td><td> </td><td> 25th August </td> | ||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P65</td><td>primerFW_ superfoldedGFP </td><td> GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACCatgcgtaaaggcgaagag </td><td> 54 </td><td> | ||
+ | </td><td></td><td> </td><td> 25th August </td> | ||
+ | |||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P66</td><td>primerRV_ superfoldedGFP </td><td> GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAtttgtacagttcatccatacca</td><td> 53 </td><td> | ||
+ | </td><td></td><td> </td><td> 25th August </td> | ||
+ | |||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P67</td><td>primerFW_mCitrine </td><td> GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACCatgagcaagggtgaggaac</td><td> 55 </td><td> | ||
+ | </td><td></td><td> </td><td> 25th August </td> | ||
+ | |||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P68</td><td>primerFW_mCFP </td><td> GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTActtatacagttcatccatgccg</td><td> 57 </td><td> | ||
+ | </td><td></td><td> </td><td> 25th August </td> | ||
+ | |||
+ | |||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P69</td><td>primerFW_mCFP | ||
+ | </td><td> GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACCatggtatctaagggtgaagagtt</td><td> 53 </td><td> | ||
+ | </td><td></td><td> </td><td> 25th August </td> | ||
+ | |||
+ | <tr style="background: #cccccc;"><td style="background: #ccffcc;"> P70</td><td>primerRV_mCFP </td><td> GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAtttgtataattcatccatgcc</td><td> 53 </td><td> | ||
+ | </td><td></td><td> </td><td> 25th August </td> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
</table> | </table> | ||
- | <h2><b> | + | <h2><b>Promotors</b> </h2> |
<br> | <br> | ||
<table class="table-fixed-size"> | <table class="table-fixed-size"> | ||
Line 854: | Line 899: | ||
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
<td style="background: #ccffcc;">A3 | <td style="background: #ccffcc;">A3 | ||
- | </td><td>mcherry | + | </td><td>pCS2+ mcherry |
</td><td>20 ng total | </td><td>20 ng total | ||
</td><td>NP | </td><td>NP | ||
- | </td><td> | + | </td><td> |
</td><td> | </td><td> | ||
Line 867: | Line 912: | ||
</td><td>coilin | </td><td>coilin | ||
</td><td> | </td><td> | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">A5 | ||
+ | </td><td>pCS2+ mcherry | ||
+ | </td><td>262 ng/ul | ||
+ | </td><td>Jo | ||
+ | </td><td>n°1 | ||
+ | </td><td>6th august | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">A6 | ||
+ | </td><td>pCS2+ mcherry | ||
+ | </td><td>264 ng/ul | ||
+ | </td><td>Jo | ||
+ | </td><td>n°2 | ||
+ | </td><td>6th august | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">A7 | ||
+ | </td><td>pCS2+ dendra2 | ||
+ | </td><td>193 ng/ul | ||
+ | </td><td>Jo | ||
+ | </td><td>n°1 | ||
+ | </td><td>6th august | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">A8 | ||
+ | </td><td>pCS2+ dendra2 | ||
+ | </td><td>340 ng/ul | ||
+ | </td><td>Jo | ||
+ | </td><td>n°2 | ||
+ | </td><td>6th august | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">A9 | ||
+ | </td><td>Nothing | ||
+ | </td><td> | ||
+ | </td><td> | ||
+ | </td><td> | ||
+ | </td><td> | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">A10 | ||
+ | </td><td>pSB1C3 (IaaM&IaaH) | ||
+ | </td><td>207,1 ng/ul | ||
+ | </td><td>KC | ||
+ | </td><td>Midiprep elution 1ml | ||
+ | </td><td>9th august | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">A11 | ||
+ | </td><td>pNHK60 (Tir1-GFP-AID) | ||
+ | </td><td>2211,9 ng/ul | ||
+ | </td><td>KC | ||
+ | </td><td>gDNA extraction phenol/chloro | ||
+ | </td><td>10th august | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">A12 | ||
+ | </td><td>pNHK60 (Tir1-GFP-AID) | ||
+ | </td><td>50 ng/ul | ||
+ | </td><td>KC | ||
+ | </td><td>dilution gDNA extraction phenol/chloro | ||
+ | </td><td>13th august | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">A13 | ||
+ | </td><td>pNHK60 (Tir1-GFP-AID) | ||
+ | </td><td>25 ng/ul | ||
+ | </td><td>KC | ||
+ | </td><td>dilution gDNA extraction phenol/chloro | ||
+ | </td><td>13th august | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">A14 | ||
+ | </td><td>pCS2+ RFP | ||
+ | </td><td>706 ng/ul | ||
+ | </td><td>KC | ||
+ | </td><td>midiprep elution 400ul | ||
+ | </td><td>29th august | ||
</table> | </table> | ||
Line 913: | Line 1,038: | ||
</td><td> 19th july | </td><td> 19th july | ||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P5 | ||
+ | </td><td>E.Coli TOP 10 | ||
+ | </td><td>pCS2+ | ||
+ | </td><td>mcherry | ||
+ | </td><td>6th August-from Aurore | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P6 | ||
+ | </td><td>E.Coli TOP 10 | ||
+ | </td><td>pCS2+ | ||
+ | </td><td>dendra2 | ||
+ | </td><td>6th August-from Aurore | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P7 | ||
+ | </td><td>E.Coli TOP 10 | ||
+ | </td><td>pSB1C3 | ||
+ | </td><td>IaaM-IaaH (K515100) | ||
+ | </td><td>8th August | ||
+ | |||
+ | <tr style="background: #cccccc;"> | ||
+ | <td style="background: #ccffcc;">P8 | ||
+ | </td><td>E.Coli TOP 10 | ||
+ | </td><td>p1 elastase | ||
+ | </td><td>promoter elastase | ||
+ | </td><td>9th August | ||
</table> | </table> |
Latest revision as of 03:15, 27 September 2012
Freezer
Primers
Code | Name | Sequence | Length | Tm | %GC | Comments | Date |
P1 | Tir1-BB-FW | cgcttctagATGACGTACTTCCCGGAGG | 19 | 57 | 58 | 13th july | |
P2 | Tir1-BB-Rv | ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC | 24 | 52 | 29 | 13th july | |
P3 | SdMTir1-BB-Fw | AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG | 65 | 72 | 43 | 13th july | |
P4 | SdMTir1-BB-Rv | TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG | 62 | 76 | 52 | 13th july | |
P5 | Tir1 SdM v02-Fw | tgcTgtgacagaggagggac | 20 | 60 | 60 | 25th july | |
P6 | Tir1 SdM v02-Rv | ctagAgcagcattagtcatttgg | 23 | 55 | 43 | 25th july | |
P7 | pCS2+BB-Fw | cgctgcagGAACTATAGTGAGTCGTATTACG | 23 | 51 | 39 | 13th july | |
P8 | pCS2+BB-Rv | tgcatGAATTCGAATCGATGGGATCC | 21 | 54 | 48 | 13th july | |
P9 | GFP-AID-BB-Fw | gaattcgcggccgcttctagagATGGCCACAACCATGGTG | 40 | 74 | 58 | 20th july | |
P10 | GFP-AID-BB-Rv | tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC | 51 | 70 | 43 | 20th july | |
P11 | p2 Hsp70-Fw | ACTGTCGACCCCGTTTAGCAGGAAATAGCC | 30 | 67 | 53 | Heat Shock | 20th july |
P12 | p2 Hsp70-Rv | ACTAAGCTTATTTGCGCTCCTTACAGTTTGC | 31 | 63 | 42 | Heat Shock | 20th july |
P13 | p2 Flk-1-Fw | ACTGTCGACCTGGACTCTGGGCTAGGG | 27 | 68 | 63 | Vasculature | 20th july |
P14 | p2 Flk-1-Rv | TCAAAGCTTGTTGACGTTTAGTCCAGGACAG | 31 | 64 | 45 | Vasculature | 20th july |
P15 | p2 ROSA-Fw | ACTGTCGACTAGATGAAGGAGAGCC | 25 | 61 | 52 | Ubiquitous | 20th july |
P16 | p2 ROSA-Rv | GATAAGCTTGGATCCCCGCAAACGCAC | 27 | 66 | 56 | Ubiquitous | 20th july |
P17 | p2 CarA-Fw | ACTGTCGACGTCCCATACCAGTACAATGCAAT | 32 | 66 | 47 | muscles | 20th july |
P18 | p2 CarA-Rv | AGTAAGCTTGTGCTGTGATTGAATTGGCTG | 30 | 63 | 43 | muscles | 20th july |
P19 | IaaH-BB-Fw | gaattcgcggccgcttctagATGCGCGAAATGATTACGC | 19 | 55 | 47 | 23th july | |
P20 | IaaH-BB-Rv | ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG | 25 | 52 | 28 | 23th july | |
P21 | IaaM-BB-Fw | gaattcgcggccgcttctagatgtttggaccggatttccc | 20 | 56 | 50 | 23th july | |
P22 | IaaM-BB-Rv | ctgcagcggccgctactagtattattagtcccccagcgc | 39 | 55/73 | 56/59 | 23th july | |
P23 | AID-Fw | cgcttctagGGAGCTGGTGCAGGCGCT | 18 | 64 | 72 | 25th july | |
P24 | Sp6 | ATTTAGGTGACACTATA | 17 | 41 | 27 | for BB detection when sequenced | NP |
P25 | T3 | AATTAACCCTCACTAAAGGG | 20 | 50 | 40 | for BB detection when sequenced | NP |
P26 | P_colPCR_pSC2+_F | CAAGTGTAGCGGTCACGCTG | 20 | 59 | 60 | 2nd august | |
P27 | P_colPCR_pSC2+_R | GCGTAATCATGGTCATAGCTG | 21 | 52 | 48 | 2nd august | |
P28 | GFP_AID_BB_Fw_v2 | TGCATgaattcgcggccgcttctagagATGGCCACAACCATGGTG | 45 | 75 | 56 | ||
P29 | IaaH_BB_Fw_v2 | TGCATgaattcgcggccgcttctagATGCGCGAAATGATTACGC | 44 | 74 | 52 | ||
P30 | IaaM_BB_Fw_v2 | TGCATgaattcgcggccgcttctagatgtttggaccggatttccc | 45 | 74 | 53 | ||
P31 | IRES_BB_F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGATCATTCAagagaTCCCC | 50 | 53/74 | 50/52 | ||
P32 | IRES_BB_R | GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgtggccatattatcatcg | 49 | 51/72 | 40/49 | ||
P33 | BBa_G1005 | gtttcttcctgcagcggccgctactagta | 29 | 67 | 55 | ||
P34 | IaaH_Kozak_BB_F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCATGCGCGAAATGATTACGC | 60 | 55/76 | 46/52 | ||
P35 | IaaM_Kozak_BB_F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCatgtttggaccggatttcc | 60 | 54/76 | 47/52 | ||
P36 | Mut_tyr1_v3_Fw | CTG CTG TGA CAG AGG AGG GAC TTG TTG CAG TAT CCT TGG GCT GTC CAA AAC TGA ACT CAC TAC TGT ACT TCT GTC ACC AAA TGA CTA ATG CTG CTC TAG TTA CTG TCG CCA AG | 113 | 71 | 48 | Primer to mutate two illegal sites in one time | 08.08 |
P37 | RFP casette GB - fw | CCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGAAGACGCTTGAGACCTCTAGACAATACGCAAACCGC | 71 | Golden brick | 16th August | ||
P38 | RFP casette GB - rev | GGTGACACCTTGCCCTTTTTTGCCGGACTGCAGGAAGACGCTGGAGACCACTAGTTATAAACGCAGAAAGGCCC | 74 | Golden brick | 16th August | ||
P39 | R0010 GB - fw | CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCACGCTCTAGAGCAATACGCAAACCGCCTCT | 71 | Golden brick | 16th August | ||
P40 | R0010 GB - rev | GGTGACACCTTGCCCTTTTTTGCCGGACGCTGCAGTACTAGTGGTCTCTGTCTTCTGTGTGAAATTGTTATCCGCTC | 77 | Golden brick | 16th August | ||
P41 | B0034 GB - fw | CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCTAGACAAAGAGGAGAAATACTAGTGAGACCCTGCAGGTCCGGCAAAAAAGGGCAAGGTGTCACC | 105 | Golden brick | 16th August | ||
P42 | B0034 GB - rev | GGTGACACCTTGCCCTTTTTTGCCGGACCTGCAGGGTCTCACTAGTATTTCTCCTCTTTGTCTAGAGACCGAATTCCCAGAAATCATCCTTAGCGAAAGCTAAGG | 105 | Golden brick | 16th August | ||
P43 | J61107 GB - fw | CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCTAGACAAAGAAGAGACTTACTAGTGAGACCCTGCAGGTCCGGCAAAAAAGGGCAAGGTGTCACC | 105 | Golden brick | 16th August | ||
P44 | J61107 GB - rev | GGTGACACCTTGCCCTTTTTTGCCGGACCTGCAGGGTCTCACTAGTAAGTCTCTTCTTTGTCTAGAGACCGAATTCCCAGAAATCATCCTTAGCGAAAGCTAAGG | 105 | Golden brick | 16th August | ||
P45 | J61117 GB - fw | CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCTAGACAAAGACATGAGTTACTAGTGAGACCCTGCAGGTCCGGCAAAAAAGGGCAAGGTGTCACC | 105 | Golden brick | 16th August | ||
P46 | J61117 GB - rev | GGTGACACCTTGCCCTTTTTTGCCGGACCTGCAGGGTCTCACTAGTAACTCATGTCTTTGTCTAGAGACCGAATTCCCAGAAATCATCCTTAGCGAAAGCTAAGG | 105 | Golden brick | 16th August | ||
P47 | E0040 GB - fw | CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCTCTAGATGCGTAAAGGAGAAGAACTTTTCAC | 72 | Golden brick | 16th August | ||
P48 | E0040 GB - rev | GGTGACACCTTGCCCTTTTTTGCCGGACCTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGTATAGTTCATCCATGCCATG | 83 | Golden brick | 16th August | ||
P49 | E1010 GB - fw | CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCTCTAGATGGCTTCCTCCGAAGACG | 65 | Golden brick | 16th August | ||
P50 | E1010 GB - rev | GGTGACACCTTGCCCTTTTTTGCCGGACCTGCAGACTAGTGGTCTCTGTCTTCTTATTAAGCACCGGTGGAGTG | 74 | Golden brick | 16th August | ||
P51 | B0015 GB - fw | CCTTAGCTTTCGCTAAGGATGATTTCTGGGAATTCGGTCTCTAGACCCAGGCATCAAATAAAACG | 65 | Golden brick | 16th August | ||
P52 | B0015 GB - rev | GGTGACACCTTGCCCTTTTTTGCCGGACCTGCAGGGTCTCCCGCTACTAGTATATAAACGCAGAAAGGCCCAC | 73 | Golden brick | 16th August | ||
P53 | GFP-AID - fw | GAATTCGCGGCCGCTTCTAGAGCCACCATGGCCACAACCATGGTG | 45 | 25th August | |||
P54 | GFP-AID-BBG-fw | CCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGCCACCATGGTGAGCAAGGGCG | 72 | 25th August | |||
P55 | GFP-AID-BBG-rev | GGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATCTGCTCTTGCACTTCTCCATC | 73 | 25th August | |||
P56 | GFP-AID-2NLS BBG-rev | GGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTAAACCTTACGTTTCTTTTTAGGGAC | 75 | 25th August | |||
P57 | pSC2+ colony primer fw | CTGGGGACGTCGGAGCAAGC | 20 | 25th August | |||
P58 | pSC2+ colony primer rv | CACTGCATTCTAGTTGTGGTTTGTCCAAACTCATC | 35 | 25th August | |||
P59 | OsTirI3myc BBG-fw | CCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCCACCATGACGTACTTCCCGGAGG | 74 | 25th August | |||
P60 | OsTirI3myc BBG-rev | GGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTAGTTCAAGTCTTCTTCTGAGATTAATTTTTG | 84 | 25th August | |||
P61 | GFP-AID-NLS GG -fw | GCGAATTCGGTCTCACGCTCTAGAGAGCCACCATGGTGAGCAAGGGCG | 48 | 25th August | |||
P62 | GFP-AID-NLS GG-rev | CCAGGTCTCACATGTTAGCAGACTTCCTCTGCCCTCTCCACTGCCAACCTTACGTTTCTTTTTAGGGACC | 70 | 25th August | |||
P63 | OSTirI-GG-fw | CCTGGTCTCTCATGCGGTGACGTCGAGGAGAATCCTGGCCCAATGACGTACTTCCCGGAGG | 61 | 25th August | |||
P64 | OSTirI-GG-rev | GGCTGCAGGGTCTCCCGCTACTAGTATTATTAGTTCAAGTCTTCTTCTGAGATTAATTTTTG | 62 | 25th August | |||
P65 | primerFW_ superfoldedGFP | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACCatgcgtaaaggcgaagag | 54 | 25th August | |||
P66 | primerRV_ superfoldedGFP | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAtttgtacagttcatccatacca | 53 | 25th August | |||
P67 | primerFW_mCitrine | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACCatgagcaagggtgaggaac | 55 | 25th August | |||
P68 | primerFW_mCFP | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTActtatacagttcatccatgccg | 57 | 25th August | |||
P69 | primerFW_mCFP | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACCatggtatctaagggtgaagagtt | 53 | 25th August | |||
P70 | primerRV_mCFP | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAtttgtataattcatccatgcc | 53 | 25th August |
Promotors
Code | Name | Length | Concentration (ng/ul) | Species of origin | Expression domain | Restriction sites (EcoRI, XbaI, SpeI, PtsI) | Reference | Sequence promotor |
Promo 1 | p2 Xlurp | 5273bp | 177,1 | Xenopus Laevis | Myeloid cells | / | Smith et al., 2002 | tctgtgtctccaaagatgccccagtagctccccatcttattttcactgcacatgctttgtactgctgtcacttactgagctcagggacccactcacaatatacagtacacatagaaatttcacagtataaggctgattagtaattaatacagataattactacatggaattacagaaaccagtgcaattagcatcagaatttaataatcagccctgtagcatcagcttatatgacaggccaacctcattttctgcttgataatttgcaacaactcctacgcttagcttctcaacagctgctcagagcccactgagcatgtgagagtcacagacactttccaagatggtgaccccctgtgacaggtttgaagtcctggatcattgctgctattgacaagctgaaactttaggctggtgcaataagttcagtatataaaacattgcaattctagccataataatttttagggtttagttctcctttaaggtggggccttgctaaagatgacttatttgcacatttataacatctgtcttaaaatagcatcttataactcatatacaaaaaaggggttatattgctacctacatgtcactcagcattaaaaagttccatgttccatgtttaagaggacatatttacaccgtttcaaacagtgaaatattctcatcttgctcgttggcaagaatgaggttaagtttgcagtgttgaataaaagataaatagtgattagctaaatttttcggcaggtttcattgtgaaaaagatgcccataggtgaaaaaaactgtgcgcaacaaaaaaattaattgtcgcccttcgccttcaatgcatttactgaccttttggcgaaacgagtcacattcgcccatcactaaagataaattaagtagtacacttccttcagtcttaaagtgcatcaacccaatgcaagatcatattgagctagaaggggaaggaatgccatatcatggataacacagggagataaatgtatctcctgtttgtagtagaggtcctatccatcagtaaaatatgtagacctctgcttcctagtggtaattgttgtacgcctcaacatccgtttctcaactagcaaagctttctcaaagcttattaccacctggagattaagcacctagatctagttattttccttgtgattgtgatatacagtatatgggacatcctagttggtaatttgcactacaggggaacaaaatggcatattcaggaatatatcagaaagaaaagcacatgcaaatagtgacaaacaatgcactgttaaaaaacaaaaaaagagaggcaggcctgttactctaggaaattcacctttaagagggagggagatataatttactaatatcagttaatttatttgaaagagaaaaagtagtaaaccccccactctctttaatacagtataaaaaaatattaaacagattgtgaacacaattaatgtggtgttaattcatatttgtgaattggtgtacaaacattcattgattaatcaaagtgcaagttcttcaatagtgcaatcaatgtccatgattttaagattaattaattatcttaaaaaaattattcaattagttcaatttttaaaagctgtaccctgcttgtaatctaattaactaaattcatctaataagtggaattaccgagtgtaattacagaaggtagcaattatattgaattaacaatatcaaattgaattcattaatttaaagtgctagtgcaaatataattaattagaaggtgaattaattcaatgaaagttaaaaatgtatgtccaggaccaacatctgaaaaaatgtaggaaaaggaaaataatagaggtggagaggtcgcaaaaacttctataagaggtttttctatgccctgggactccacacaatggagaagacaaacactggggactgtggtctcaaattaaccgtatctatctcattgtggagaggctaagctgtactataaaaccacagatccttcagtgtcattgaaagtaaggagaatttaaaggaagagaaaatcaaatgcggcacttgaaaggctaacagcgctggatattaattaaaagaaccaagccaattctaaaaagggtttggtagagaacagatttgttaaaatcaatttgaaagttaggagactccgccaatgcactgtaataagaaaaagtatatcagtttgcaggaaaacggagaaggcaaaactgggtggctaaaagaagtgagaaaaagaaggactagaaatgaaggggtggaaacctgagtggtgtagtaaaaagcaacaataaataaaaaaacacaacatagtgaaaatattttattgccatctgtttattaaaatctgtctattctgcacctgcaattctaaaatcattgtattctgaacagctgatattgcattgctctgttggaacttgaatggcggccctgtggctatttaagcttaattctataaacaatattagaaactcgaaaatgaacacacagaatttaccagtagaggattaaagtttattttatgtaaatgaatacaaaattcttactgtttttggttgctattggccgtttagatattttttaagcaaaacattagcaaagccctcaaagttaataattcatcggttaggccgtcctttattgatccatcaatcaggattcagttttgtgctgtcaaaaacaatgtgcgtgggaacaactttatgtggagcagggactcttcatgctctggtcagaaaatgtacaggaacttggaacagagaagaagtataaagaaaataatagcatgcatcacaatgtttctggtgtgccaatgaatagctgtgtgttacaaaatctctgtcttgtataaagacaacaccagctacagtaagttaaattctaaacacatatttgtccaatacaggggcccaattaatactgcttgtgcttttacggcacaatcaatctttaagaaaacccctcaagagtttcctaactcacgctggttttcatgctgaaaaagaaagctttgtaagttacccgacctgctgcaggactttatcccagggtgtattaataggagtttttacaataatggctcatggtaatactctgattaaactcaatagctcattgcaatactttgataaagaactgtataaaaatataacacaaagaactgttctgctgttgaacccgatgtcgtatgttgagcccaatatagagtaaaaagacagctttataacgtataattgtgtataacaaagctttgtatgatagtatacaaggcagccttataatccataatcctgtataatgaagcgttgtgcaagagaaatcatgtattatctaatatgcttatgatttctgaagcccttatcagactcttctttattataaataggggcacaatatgctattcagctaccagctagctaagataagatctctgtttcggatgtgcttgtgggaaacaagatctgttattaaccaaatcattatgagttattgactaagcctctcaatgagcttactgtttgaaagcatatataaactgggggtcaccatttggtcagttgagatctcacttgtggggttgacgcgtgccgccacaggaccttccgtgctccgtttgagggattcaatctgctgactgttccaggatctcccagacttggttgcagattgtttgctttgctcttttactcattcggtaatgtatatcttcttcattctatgtatgtaataaacctttgcttatactagctggtgtgtttgaattacttccgagagactcatagaaccacaattaaaacacagggtaacaaagtctgggcacccctgagctgacatagggcatctattgtctttgtccacagtcctgatatttttttatcaagcagtaactatgggcatgcaatttgcagtggggaaaacaagaatttggattggtgctagaactgtgtgctgctgaacaacaatgaaaactgccatcttaattctgctgaactgacacagctattttcttttttaaatatgtctattagttaaggggttaaactttaaaatgatgtagagagtaatatcccgagacaatttgctattggtttttattttttttactatttatggtatttgagctatttcgctttttattcagcagctctccagtttgcaattctggcgatctggttattaggatccaatgggtcagtgacccctgtctgaaagctgaaaagcctcctaagaagtaggcaagtcatttaaaaaacgatataaaaaaataaaataatgaagaccaagtgaaaagttgcttagaagtggccaatctatatcatacttaaagggatattgtcatgggaaaaacatttttttcaaaatgaatcagttaatagtgctgctccatcagaattctgcactgaaatccatttctcaaaagagcaaacagatttttttatattcaattttgaaatttgacatggggctagacatattgtcaatttcccagctgccccaagtcatgtgacttgtgctctgataaactttaatcactctttactgctgtactgcaagttagagtgatatcaccccctccctttttccccacagcagccaaacaaaagaacaaagggaaggtaatcagatagcagctccttaacacaagataacagctgcctggttagatataagaacaacactcaatagtaaaaacccatgtcccactgagacacattcagttacattgagaaggaaaaacagcagcctgccagaaagcatttctctctaaagtgcaggcacaagtcacatgacttggggcagctgggaaattgacaaaatgtctagccccatgtcagatttcaaaattgaatataaaaaaatctgtttgctcttttgagaaatggatttcagtgcaaaattctgctggagaagcactattaactatttgaaaaaaacatgttttctgatgacagtatccctttaaagttgacttcatggtaaaccacccctttgaaaacattacagactgtcagaaaagtatgtataaatggggaaaatatcaggaccacctaatttgagatttggtaggtatgtcactgtttgttacttttttcggttagtgaccttgcaacatttcctgtaacagcttatttcagtgtttacccttgttgatcaagggttacacaaattcccagaaatataacgtgcctttagtgattgtgcaatccatatagataaaaggggatcttgctggcagagttcacagaagagctgacttagaaacccact |
Promo 2 | p2 CMV | 1016bp | 65,3 | Cytomegalovirus | Widespread | / | Werdien et al., 2001 | CATAGCCAATTCAATATGGCGTATATGGACTCATGCCAATTCAATATGGTGGATCTGGACCTGTGCCAATTCAATATGGCGTATATGGACTCGTGCCAATTCAATATGGTGGATCTGGACCCCAGCCAATTCAATATGGCGGACTTGGCACCATGCCAATTCAATATGGCGGACTTGGCACTGTGCCAACTGGGGAGGGGTCTACTTGGCACGGTGCCAAGTTTGAGGAGGGGTCTTGGCCCTGTGCCAAGTCCGCCATATTGAATTGGCATGGTGCCAATAATGGCGGCCATATTGGCTATATGCCAGGATCAATATATAGGCAATATCCAATATGGCCCTATGCCAATATGGCTATTGGCCAGGTTCAATACTATGTATTGGCCCTATGCCATATAGTATTCCATATATGGGTTTTCCTATTGACGTAGATAGCCCCTCCCAATGGGCGGTCCCATATACCATATATGGGGCTTCCTAATACCGCCCATAGCCACTCCCCCATTGACGTCAATGGTCTCTATATATGGTCTTTCCTATTGACGTCATATGGGCGGTCCTATTGACGTATATGGCGCCTCCCCCATTGACGTCAATTACGGTAAATGGCCCGCCTGGCTCAATGCCCATTGACGTCAATAGGACCACCCACCATTGACGTCAATGGGATGGCTCATTGCCCATTCATATCCGTTCTCACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCACTTGGCAGTACATCAATATCTATTAATAGTAACTTGGCAAGTACATTACTATTGGAAGGACGCCAGGGTACATTGGCAGTACTCCCATTGACGTCAATGGCGGTAAATGGCCCGCGATGGCTGCCAAGTACATCCCCATTGACGTCAATGGGGAGGGGCAATGACGCAAATGGGCGTTCCATTGACGTAAATGGGCGGTAGGCGTGCCTAATGGGAGGTCTATATAAGCAATGCTCGTTTAGGGAACCGCCATTCTGCCTGGGGACGTCGGAGCAAGCTTG |
Promo 3 | p2 Flk-1 | 2723bp | 54 | Xenopus Laevis | Vasculature | / | Doherty et al., 2007 | CTGGACTCTGGGCTAGGGCAATTGTTCCAGCCTGACCCCTAAAATAGCTCTGAGAATATGCAATTGGTGCAGGTTGCTCAACCTGTGAATACTGTCAGGCCTGGATTCCAGGCTGAGGCACAATCTGCCATTGTTTTTGCACTTTGCACCCTGCCCTGCACTACTCTAAATTTCTTCAGGTCTCTGAGAATTACAGAAACCTGCTTTCCGCCCCATGAATACAGTGCTACCTGCATTCAGCAGTGCTGTATTCATGATTGGTGTGTGAAGAGACATAGGCATCCTTCTTCTCATTCAGGTCACAATGGAGCCCTGTACTTACCACCCTGCCTTTTGCGTCCCTTTATTCTTTAGTACTTGCATCTGAGGTTATAATAAGAGTGGTTGTTTAACTTTGACCCCAGACACAAATACTAGACCACTAGGGCTCATTTAAAAAGCACTTGCATCTGGGGTCTGGCAGCACTCTGCCCTTAGTGCTGGACTGGAGTAAGCATGCCAGCCCTCCCTACAGGGTTGCCAGATTGTCAACCCTGTAGGGAGGGTTGCCAGATTGGCGGGTTACCAGGTTTATAGCCAAATTGGGCTATTTTTAAAACTAATTTTTAAAAGCGGGCAATTTTTTTATGCAAATTGACGTGACATCAGAGTTCAAGTGATGGCAGCCAGTGATGGCCTGCCCCACTGACCCAGCTCCTATTTTGCTGTACTGGATAGTGGTGCTGGAGGAATCTGCTTGACTGAGATAGCCCAATAACAGTGCTACCCTCCGGACTCCCGGAAGCCCTGCTTTGGTTTCAACTGCCAGTCTCTGTGAATGTGTTACCTGTATAAGGTATGGAAAATGGAATTGGCAAATGAGGGCTGGCTGTGGGACTAACGGAATGGTTCTGTGCCACTGCGTCTGGCGAGTCCGGACATGGAAAAGACCCTACTTGTATTGCAATTTGCAATTGGGTTCAGTCTGGTATACGAGCTGAGCAAGTAGTCTAAAGTAGTCTGTGTGATACCCTTTCATACCCTTATTGTTTTACTTTATTTTATTAAGATTTTTCAGTGTATCAATTGGGATATTTTTGGGCTACCTTTAAAATGGCTTTTGGCAACTTTTTGGCTATTTTTTTTATAGTCTTTGGTTGGTTTTGAAATGTAAACCCGACTCCCTAATTTGTGCAAGGTAGGCAGGGTCAGACTGGGGGACCCGGGGCTCACCGGGGCTTCTGCCTCAGGCCCCCCCTCCGGACCCCGGGGGATGCTGCCTCAACTGCTCCGGACGCGCAAGAGTGCACTTGCGCATGCTGCTTTTTTTTGCTGCAAGGAAGAAAACCAGGGTGCTTATATGGGCCAGTGGGGCCCATTGTTTTTTTTCCCGGTGTCCCACCGGCCCAGTCCATCCTCGAAGGTAGGGAATGGGAGAGGGGAAGTGTATATGTGGCCTGCCCTCACCCCATGACTACAGTCTAAACTGTATTCATGTGGCACACACACAGGTCTCTGAAGACATAATTTTGCCATATGAATAGTGACTTGGCACAAACTGCTATATCTCGTGCTGGTGTGGTCGGTCCTTTTGTGCCAGTTTGTTGGCACATGTGCTGCAAGTTGCAAGGAGGCACTTCATTCAGCCCAGTAGAAAACAGTTTTTGCTTCAGGAATGGGCAGCACTTCTATGAATGATCAGGCCCTGATTTGTGGAAAGGCCACCTAGTCCTGGGCCTAGGGCAGCAGGATTTTAGGGGGGTGGTTCCAACCATACCCACATTGGTTAAAAAACACTGGGGATGCGGTGGAGATATAATAATTTTTTACATTTCCAGTGCGCCAATGAAATATTTGTAGGGACAGGGGCCGACGAACGGCAGTGGGCCTAGGGGCTCCTGGATGTAAATCCAGCCCTGCGAATGAATATATATAGTGAATAAAGTACCCGCTCTTGTAAAATATAAGGAAATTATAAGTTACTGAGGAGGTAACTTCTAATATCCTCATATTTTGCAACTGGGGGTACTTTATTTATTATAATACACAAGTTTCAGTGAGTCATGTGACAGAAATGACATCAGAACTCAACATTTATAACTGATGACATCAGAAGTCACCGTTTAGAAGGATATAATTTACAAGATATTCATGGCTTTTGTGTATATATATATATATATATATATATATATATATATATATATATATATAATGTATTAATTACTGAAATGTAGTGCTCACATTCTCCTCACAATTTATTTTGAACTTTCGTACATACACCCCGAGGCGTAAAATGACGTGTACTGCTGTTGGGTATGAAGGTCTGTGACCACTGATAAAAATAAAGAGGGATTATTGAGGGGTTTCAAAGACACCCCCGATATAGATACAGAGAAGAGCAGCGAAACAGCCACAAGCAAACGACTCGCTGGAAATCCGAGAGGAAGGGCGGGAATTTGGCAGTGAGGGCGTGGCTTGCGATGCAAGTTGTGAAGGTGCCGCTATGTAGAGAGCACAGGCTTCATGAGAAGCGCTGGAGTTACATCCTCTGTGTGAGCATCTCCGGGATTTCTATCGCAGAGACCTGTCAGTGTTGCTGCTGCTGCCTCTTCCCTCCTCCTGAGCGCTGCCTTCGGGGCCCTTAATCTCATCACAGTCCATGTCTGTAGCATCACTGGCTGCTGCCCTCGTATCTTGCTGCCAATCGCTGGGACACTGCTGTCTGTCCTGGACTAAACGTCAAC |
Promo 4 | p2 14xUAS E1b-ATG | 454bp | 93,7 | Xba1 | AAGCTTAGGCCTCCAAGGCGGAGTACTGTCCTCCGGGCTGGCGGAGTACTGTCCTCCGGCAAGGTCGGAGTACTGTCCTCCGACACTAGAGGTCGGAGTACTGTCCTCCGACGCAAGGCGGAGTACTGTCCTCCGGGCTGCGGAGTACTGTCCTCCGGCAAGGTCGGAGTACTGTCCTCCGACACTAGAGGTCGGAGTACTGTCCTCCGACGCAAGGTCGGAGTACTGTCCTCCGACACTAGAGGTCGGAGTACTGTCCTCCGACGCAAGGTCGGAGTACTGTCCTCCGACACTAGAGGTCGGAGTACTGTCCTCCGACGCAAGGCGGAGTACTGTCCTCCGGGCTGGCGGAGTACTGTCCTCCGGCAAGGGTCGACTCTAGAGGGTATATAATGGATCCCATCGCGTCTCAGCCTCACTTTGAGCTCCTCCACACGAATTCCCTCGACGGTAC | |||
Promo 5 | p2 ROSA26 | 814bp | 55,7 | Mus musculus | Widespread | / | Gross et al., 2006 | gtcgacTAGATGAAGGAGAGCCTTTCTCTCTGGGCAAGAGCGGTGCAATGGTGTGTAAAGGTAGCTGAGAAGACGAAAAGGGCAAGCATCTTCCTGCTACCAGGCTGGGGAGGCCCAGGCCCACGACCCCGAGGAGAGGGAACGCAGGGAGACTGAGGTGACCCTTCTTTCCCCCGGGGCCCGGTCGTGTGGTTCGGTGTCTCTTTTCTGTTGGACCCTTACCTTGACCCAGGCGCTGCCGGGGCCTGGGCCCGGGCTGCGGCGCACGGCACTCCCGGGAGGCAGCGAGACTCGAGTTAGGCCCAACGCGGCGCCACGGCGTTTCCTGGCCGGGAATGGCCCGTACCCGTGAGGTGGGGGTGGGGGGCAGAAAAGGCGGAGCGAGCCCGAGGCGGGGAGGGGGAGGGCCAGGGGCGGAGGGGGCCGGCACTACTGTGTTGGCGGACTGGCGGGACTAGGGCTGCGTGAGTCTCTGAGCGCAGGCGGGCGGCGGCCGCCCCTCCCCCGGCGGCGGCAGCGGCGGCAGCGGCGGCAGCTCACTCAGCCCGCTGCCCGAGCGGAAACGCCACTGACCGCACGGGGATTCCCAGTGCCGGCGCCAGGGGCACGCGGGACACGCCCCCTCCCGCCGCGCCATTGGCCTCTCCGCCCACCGCCCCACACTTATTGGCCGGTGCGCCGCCAATCAGCGGAGGCTGCCGGGGCCGCCTAAAGAAGAGGCTGTGCTTTGGGGCTCCGGCTCCTCAGAGAGCCTCGGCTAGgtaggggatcgggactctggcgggagggcggcttggtgcgtttgcggGGATCC |
Promo 6 | p2 foxi short | 2020bp | 105,9 | Xenopus Tropicalis | Ionocytes | / | Unpublished results | GTGCCTCCGATCAATTCAGAAGCATGTTATTGTTTTGCTTCCATTACAGGAGGCTGGAGACGTTGATAATTAGACACTAACATATCATTACATCATGAATTTAAATGACCTTTCCTTTATCCCTTCCTTTATTCCTTTTAACAGTAGATGTTGCTACTAAAATACTTTTACGGCAGGCAGCACTCTTCATTGATCATTTGTTAATGAGGATGTATTGTGAGAGCTGAATCTAATGTCTGCCCACCAAACAAACACAGACATCTATTTATCACAAAGCTGGTCCCTGCATGAATAGCAGCACACTCAGCATATGAAAACAGGGTCAGTTCCAAAGCAACTGAATACATTTATGTCATTATTTATTTCATATAATATGTGCACCCCAGCAGGATCAACCTCAACTTTATCCTGGCAGGTCCAAATATCCCAAAAGTAGCTAAATGAATACAGGTATAGGACCCATTATCCAGAATGCTTGGGGCCAAGGGTATTCCGGATAAGGGGTCTTTCTGTAATTTGGATCTCCATACCTTAAGTCTACTAAAAAAATCAATAAAACATTAATTAAACCCAATAGGATTGGTTTGCATTCAATAAGGATTATTTATATCTTAGTTGGGATCAATTTCAAGGTTCTGTTTTATTACTACCGAGAAAAAGGAAATCAGTTTTAAAATGATGAATTATTTGATTAAAATGGAGTCTATGGGAGACAGGCTTTCTGTAATTTGGAGCTTTCTGGATAACGGGTTTCCGGATAAGAGATCCCATACCTGTACACCAGATTTGTTGTAAATTAATCTCCCCATTTAATTTTATGTTTGTATTATTTATAAATACATGAAACAATATTACTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCATCTATCTATCTATCAAAGAAGCAAAACAAAGAATAGGACAGGCAACACAGGGATGTAATGCTACTAATAAAACTTTGCTTTACCTTTAAAAGATGAATTTCTTGCAGTTTTGCAATTCTTCATCCTTCTCCACTAGTGGGAGTTAGTTCTGTAGCAAACAGGTATGTAAGGGATTTTAGCTATCATGCTTATTTTACAAATAAACCATGAGTCTTTCCCCCTCCTTCTTAAAGAGGGTGTTAGCATATTTGTAGCCAGTATCAATTTCCAATGAACATTCCTGTTGCTTCCATTAGGGTTTTAAAATATGCTGTGTTCGGTAGCTAGCAGGAGCAATAGGATTATATAAATGACAGATGATATAGATATAGATGATATAGATGTACCAAAAGTTTATACAATATCAAAGCTGCCTTACATGTTATTACTTTCTCTGGCTGCAACTGTTCCAAAGACTGCACTTACAATACAAACAGCCCTCTTTTGTTACGTGTAAATGGCTTGTGGGCCAGCTGAATACCAGAGAATATATAAAAGAAGAAATAAATATATATATTTATACAAGTAGGAGTTCAGAAAGTTGTAAGGTAGCCATGAGATGTAAGATTTAGCACATCTCAGTAAACTGAATAAAATGAATTCTTATGGCTTGGTTCAGTACCATGAAAACATTCTGTCCAGTAAATGTTGATCCTTATCTTAACTTTATAATGCCATTAAACATAGGGGAAAAAAGCGAACAAACAAACAGATACAAGCATACTGTGATAGTAAATGTTTTACTTCTTGGTTCTGCCTTTTGTGACATGGGGGAATTCAATGATCAGGTCTGACAAAAAAAAAAAAATGTGAGAGGTAAAAGAAAACACACACACACACACATGAAACACATATATCCCATTCATTTAAAAGATAAACACACATAAATTTGAATTTGGCTTATGAGCTGCAAGCATTCCAAAAAGGGATATCAGTATAATCTCCAACCATTCAGGGAGGCTGGGCGGAGCCACAGTGAAATCAAAACACAGCAGGTGTATAAGTAGTGGTGAGGAAGGGCACAAGATATACACCTGCTGCAAGACATTAGCTAGTTCAGGTGCT |
Promo 7 | p2 UASt | 341bp | 64,8 | Saccharomyces cerevisiae | GAL4 inductible | / | GTCGGAGTACTGTCCTCCGAGCGGAGTACTGTCCTCCGAGCGGAGTACTGTCCTCCGAGCGGAGTACTGTCCTCCGAGCGGAGTACTGTCCTCCGAGCGGAGACTCTAGCGAGCGCCGGAGTATAAATAGAGGCGCTTCGTCTACGGAGCGACAATTCAATTCAAACAAGCAAAGTGAACACGTCGCTAAGCGAAAGCTAAGCAAATAAACAAGCGCAGCTGAACAAGCTAAACAATCTGCAGTAAAGTGCAAGTTAAAGTGAATCAATTAAAAGTAACCAGCAACCAAGTAAATCAACTGCAACTACTGAAATCTGCCAAGAAGTAATTATTGAATACAA | |
Promo 8 | p2 HB9 | 3142bp | 48,1 | Danio rerio | Motor neurons | PtsI | Flanagan-Street et al., 2005 | GCCAGTCGACGATATCTTAAGAGCTCACGCGTAAGCTTAGATCTGAATTCATTTAAATTAGCCTGGCATCTGGACATCGTCAATCACGGGCTCGTGACACCGAGAGGGGACTCGTGGGTCATCTCGTTTAAAGCGCATTTTAAATTACACCAGCCACTGAATTGTGTGTATTCATCACATTAGTGTGTACCAGTGTGTTCGGTTAAGTAACACCTGGTTTTCGGGCTGGTTCATCAGTTTGTTTGGTCCAGTGAATCACTTAAGAGATAATCAGGCTTATCAAAGCAAACTGAGGAAAACGCTTTCGACTACAGAAGGACTTTGTATTATGCAATGTTTTCAAAAAAATATCTTCATATTCAGTGTGGGAGATAATAAGAAAGGAAAAAGCATGAAGCACTGAATCGGTGTCCTTTAATGACTTTTATAGTTGGGAACAATTAGCCGTTCTCCACAAATTAAATTGATAAAAAGATTACGAGACCCAAATGCGTTTAAAAGCTGTGGTACGCACGGCCCATTTGTGCAATTATGATCGAAAATATTTATTGCAATAAAAGAGACAGTGCAGGTCCCGTTAAATTGGCTTTTCATAATGAACACATTTAACATGGATAATTAATATATAAACTATTTCGATTTGTCACGATGATATGTGTTGCACTTAATTGTGAAAGTAATTACCGAGCGAATGAACATCTTTGAGGAGAGTCCCTGGTTTCAGGCCCTCGTATCATCGGGTGCAATAATAATTTTACTCTCTCCTGATGTGATGAAAAAGAGCCATAAATCTCTCAGATCGCTGCAGGTCCACGGCTTCATATTTCACCGCTGCTTTAATAGAAATATTCATGCGATCCCTTAATGAAATTAAAACTGAGAAGCGGACAAAGCGGCCCTAGGCTATGTGCAGCGTGTAAAGACACAAGAGCATTTGATTGGGAAAATGTTGCATCCTTCAAATATGAGGATGTTGTTGCACTGTAAACCACGTAAAACATTTTTTTTCTTACAGTCCAGTATTGGCCACACTTGATAATTGTTACATTTGTTATCAATTACACGCGAGGCTCATTGAATGTCATTTCAGAATTGGTCAGTGCATGCGTCATTACGGCACGCTGTTATTCTCATCCTTTCGGCCGGTGACTTGTTGATTTCCCTCGAGCAAATAAACGCATGCGACCAGTCTCTCGAGAGTGGTTAGCTGATGAATTGAGGAAAACTAATCCGCTTTATTGGTAGACAGGTTAAGGGCAACAGAGTGTCAATAATTCTCTCCCTGACCTCTTTCTCCATTAGCTTAAAGAGGCTTATAAGAGGAACAGACCCCACAACATGACACCGATCCTCCACATCACCATCTCACATGTAGCCAACCTAGGCTTGGAAAATCATTTCGATTTATAAAGCTCAAGATCCAACATTTGCCAAAAATTCATGAAAACAGGGCACAAATAGGGCATCATTTTCTCACAAAGTATTATATAGGCCTAATTAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGACAGATAGATAGATAGAAAAAAAACAAGATTTTGCTTGTGATTCATTATGCATAATAATTTGCCTATGTTTATTAATAAACATCGTGTTAGGATTATAAATGTGTTGTGATGAATGTGATTAAATGTTTAAATATTTACTGACAGTTGTTAGCCTATATTTACAGATGTAAAAAATCTACGTCTAATTAGTATGTGTTTCTTAAATCTATAATAAGGAAAGGCTTATTAAGTTTATCTGTACAATTTAGAACAATTTTACGAATTTATCCTTTAGAGAAAAAAAATGTAATAACACCATATTTAAATATCCTGTTGAGAAATTATTAAATCAAATTTTTACATTCTAAGAGCAAGGTTAAATATTGAGTGAAAGGTTGAATTGTTTTTAAGACAGTTGTTTTATTTTTTTAAGCATATTGTGGTGGGTGAGTTTTCAAAGATTTACACATTTCAAACAATTTATTAAAACAATATTTGTTGCATAAAACACTGATTTGGTCAGTTTTACCACTGACTTTAACTTATTTATTTAATTAATTATATTTCTATTGTTCATTGTTATTAAATTTTGCCTACCTAATGCTCTCTAACAAATAAGGAATAACTTGAATATTTCCTCTGCGCAAAATGTTCATCGTAATGTAAAAGATCCGTTTGACATTTAAAATGGTTCTCTTCTCATAAAGATAGTAATTCTTTTAAGAACTGTCCTCTAGAAGGTTCTTTAGGGAAACAAAAAATATACGTGTACATCAATGTGAAAATCAAATAGTTTTTTTTATTATTGCAGGCTGTTCAGATAAAAAATACAATTGAATAAAAAAGTATGGACATTAAATAGCAAAATGACAAATGCGTGTATAGACTATGTTATTTTGTTAATTCATTTGATGAAATAAAAAAAAATTATACAAATGAAAAAATGTCATGCCTCTACATCTATGCGAATAAAAAGCATGGCTGTTTTTTTTTTTTTTTTTTTTGGTCAAACAAACTATTTCGCCAAAATAATATTTTCGAGTTAACATGCTTTATCAATCTTCGTTTATATTAAGATTATAGGTCAGTGTTTAAATAATGAAATCTTGATAACTTTATAAATGTTTCATGTTTCAAAGTTGCATGTTAATAATTGCCCTTAGACTCTACAAATCTGGAATTATTCATAAATTAAGTAGATGTAAAATCTATGTGTGCATGTTGTGTTTCCCACTTAAAAAGCTTTCATTTTATGGGAGAACATACTTATGACAACTCTTGAACTTCTACAAGCACACCGTGAACACTGGAATCACTGTCCAATCGGAAATGCACAAGGATGACGTGAATGACCAATCCACTCCCTCTCGGGCAGCAGTTGACAAGTGGACTTCTGGCTTGCACACCTTGGCCAAGATCATAACTTTACGAAGAGGAGCGGTGACACTCCTGACAGCCTTTTAAAGTTACGAACGAGGCGTTAATCTGTTTGTGAGGTGGGCCAG |
Promo 9 | p2 pax6 | 1957bp | 58,9 | EcoRI | ctagcgggggcttgaaaggaagagccggggcatttgcatgagaaggggcctcaatgggcagaggaagccactgggctgcagggagttgctgggcgagtcagaggggccagggaggcagcagctcattatccagctcccattcaggagacctcctttcagtcacatgcaaactttctggcagagtcgtttgtctcctcattgtactttatttgggaatgtaatggctgtcaggcccctcattcactcgccacatactttattgatgagccgcgacttctgccactttcacacgtcaagtaaagtcaagtgtataatctttttacttcatttctcccttcccttccattgctttgcacccagcctttctctcctgcttcttcatttagctgcatttgtttttttacccctctgcttcttagtattagaccccctccttcccttattaatgtcttggaattagggcaccctgagatcaagctggtactttaaatgtcacctttaaagcttgcacccaggggtttctttggtgtctactgaggtgtatcctaatagtgaaccattcaacaagtgtaactttgtggttgtgtgtgtggtcaaaacactaaagtcatttacttattgacagctccagaggggtcacctttagtgctttcctattggcagagaaagctgttttagggtgctgtgacgtcatttcgatgatgttataaaatgtatttgcaaaggattagggcaactggtattagaaaattgtgattacttagaccaattgtatttgagagtaaaaaatgtagtcacaattgtaaaaataatataattataaaaattataaataataataataataattaaagggcagataaacgaaatgttacatttaattagcactaaggactgtggaactgtagctctgtacaaattagaaataaaatactcacctacacgcgaatgaattttccccatctggaattgattgcaaaaataaatttaaaaaaataaaaataaaaatttgcctgaagaatttgcttcacttgaggtggaataataaaaaaaaaaaactaaacaaatggcacgtggggagaggttggtaaatgtttgctgagtcccacttcagcctccttctcaagggttctctctatcaacagagtaaattgggcatattctcactttctcagagggtccttgcaggttgaaaaaagaaaagaaaatgaaaaaaaaaaagaaagtaaaaaaaaaaaactaagagcttgggggcaattgagagtttacaaagcgcctgtcttacagatatttcttgctttttttacatgtgaatgttattttgctttatcccctattgttgccgaggggggagcttttctgccaaaatgcaaatcgcctccattcacagacaaaatcgcctgtacaacagaacccagggcttacaaggaaaatgatttaattcccattgtgtgcaagtacacaattattacagttgcagggtgagctctggtgaggctggaaaacaagggggtacgaattgcgacaagcgacgcgtaaaaaaagttgtcttcgctgcgacttggagcgattttttgtaaaaaaaaaaaaaaaatacaaaaaaattattttttccaaaaagtataaatccaatattgatggaaaaatcaaacattaagaaaaaaaactgctgaaattcttgtaaccaacaccaacagagatttcatatcgcctaaaaaaaaaaaaattctcagcactgaagaagggaaagagagagaaagaggaaggGGAAAAAAAAATCCAAGTGTATTCATATTCAAACCAGTGAACCAATGGAACTGGCCTTTAGGGAGGCGCAGCCAATTGGCAGCTAGGAGGCACTGCTTGCGCTTTGCATAAAGCAATATTTGGTGTGTGCAGAAAGCTTGCTTGTTCTTTTTGCAGAAGCTCAGAATAAACGCTCAACTTTGGCAGATCT | |||
Promo 10 | p2 NBT | 3481bp | 168,7 | Xenopus Laevis | Differentiated neurons | EcoRI | Huang et et al., 2007 | tctagaccctgtctgttcctgattacatctagtttgctgcctgtccggattattgcctgttttttgactactctcttgtatcctgtggtgtactttctattttggtgtgtttgactcggcctgtgaccccaaccatttatttgtctcctgtaactgtaccgcattgactgattgttactgacccggcctggcttttaactacaattcttgtttccaattctacaaccccacacttggttcccttgcttggtcagaactctctccttgggcctctcacgttaagacctggcgtcatctgagtagcagagggctagtcccgatgccaaaggcggctgttataggcagaagggtgagccgcgactgggtccttggtgtctgttctgattttgggacaccctgccttacagcccccctcaagtctctgattattgccttgtaaccttgtaaccaatcagtggaaatcaagagacctgcaaagcaggaagtagtgttctacttatgtcactccagtctttatacattacatttttggctaactaactatattagaaacattttttattttgcatagccaatctacagtatttacccagtttttatttttaaactgaataattattttaaggataatatctgcctgcagaataaataattcgatatatgccatggataagcaatatggtgtcttgacaagacagaatataatttgatattttagaagcatggcttttatggacttatacacatgtaggtatgatgtgagaacctggttaatggtgtccatttgctgtcatgagtttgcttggttactgagcacagactattttatgagctttgccaggtcctgcttttctcatcctttcaaaacaatgtgttcccttcactccacacagagtaatgtccatgttcctggtgtgtgagtctcctcttccaaatgcagcatcttctccccccccatgccatgtgacactttcattagcatctcctcatgctttgtgtgaattctgctctgccctacccctccccttatgcagcacaaggaagaagggagaaagctgtgtctgcatatctgcaaaaggctgcctgactgacagtaagaatgtctgtctcgaggacctcttcccacacagctgtaccactgaaatccatatttattggtagtacatctgttactaacattttaacattaacaacagtgattacattaccttcagaacattgcagtaaggaacagactcctgtatgctcatagtgtaataccaggagaatatattgtaaatcttgggtgttgcccactttcagtgatatcagctttgctttctgtattttttaatcatttaatgctccttcatagtaatcttgaaatgcaaacattttccttaaggtgggcatagccaaacaatattcaacatcaacacactgggccaaatcaggatgatctgatcaacaggctaacaatcagatcatatttgggagccatttgccaatgtggtcctcttccaacatgattatccaaactgtctgatagatatatctgcttgatagtcaaatattggtcaggcaggccattgagaaggcctcatgcatgggccaataagctgctgattgggttgcctacttgtggatattttactagcctggccagaaaaaatgttgcttgatgccaatgtcaataattggaaaaaaacattaaaatataagaaggctgttacttttttcccagaaaaggtggtaaccataactgccaactaattctggaagagctgagttggcagcttatacttggccatttatcaatcaccacctataaggatgtaccgagtctgcgtgcttattaggtatatgtgagtatgacacaattttaaacactattatgtctgcaagagctgagtcagcagttatacttggccatgtattgccacccataaggatgtactgattcaatcatttttctccaaacccctaaattcatatgatgtctgccagcttattaggtacagtatatgtgagtatgataaaaatatttgtaatatttaatattggttgtgttttctctctatagaaataatacacaatccaaacaaaagtggcataaatgtgatatattgtaatgacatgataaggaaattacactgatttattggctattaatatttctcatttgcaatcactatgctgctctggcagcttttatcagcacatcagtgctacagaaagaatggaaccctggctgtcatgctgattctaatgctcagcagcattttacacattttgctgcattttctagctatcagctgattactcccaggatcccccttgtgttccactgcagatgtatttactgatgcttagcttctaccaacgggcacttacaagtatttctatgtgggtgtaactagaaagaagatggactatgtaaagcatatgggcctctagtttgggcataaccttaatgtacatttggatgggaggttccagggtaaaggttaatccaatagcactggtttcccctagctttgccacttttaataggcaaggtttcagccctgtctactcatatgtaaattgtgattgtgattttatttcacatttttgttctgtatgtttggacagatgtcagtaaaaccatttaactgcccccgtttgttcaacctcctcgttcttctgctacatccactggtctttacggaagcaattaaataaattaagggccatatgatttcctgccctgtaaaactggtgtgtgtttgcggtctgaggcagatgtgctgcttccatttgaatacaggggggatattgaaacactgactcgggaatcccctggagagtgatttgaggcagaaatggatatggagcagtatcatgtgataaaatgaaattgtgaggcagctgcatagggtggcacccatccccactgcaccttccttccccctacagctcccagtgatggacgagttgtgtgggggatggtacctgtcactaatacctttggagtcatcattaggctaatattatcaataacaaggttaatgtttctataggcttaatcagcttctcattcgtgttttaattagaattttttttcattatttcagtataatattcctttgtaataagcaataagcacattcccagcatctttttcaccttcttggtggtatctgcctgatggtgagacaatgcagcatccatttccattccctcccttgtcatttgattggttcccatccctacaatgctcagggccagtggctccacccaccattacatcatctgagcccaggaccacagcaagtgaataggagagagcagcaccctccccaaggcgtcacaagtgtataaaaggcagcagaaggagcctctgacagcccagattcttagccggctggaaaccatcggggtgttgcacaaagggcaagtaaagcaacaagaaagggccacaccatccactgattacaccactgtaagactcaacccaatc |
Promo 11 | p2 LMO2 long | 4730bp | 170,1 | TTAATCTTGTTACCAATTTTATACCAAATACATGATATCTGAGTGGCATCCTCTTAAAGAGACACTTATAAAACAGAAAGTGTTAAATGTAAAGTAGCACAGTTTTAGGGAAGAAAACATATACTTTACTTCTAAAAAACAGTAATATTTTAGTGCTTACATGCGGAAAAGTAAAGGGTTAAACTGGCCACACAAGAGATGCTCCACTTGTGTGACGTTGGTGCCAAACAAGTGTATTGTCTAGCCCTCAGATCACTTAAAGACTCAGTGAGAACTCATGCAATTTCCTGCACAGCCCATTATGGTCTCATGGGATTGTCCAACCCTCCAAAAGCTGAATGAGCCCCAAGCAGATATCAGTTGGGAAGGCCATCATATAGCCCAATACATGTACCACTAAGATGCCAGCTTGGTCAGGAGCAGCCCAGCCTATGAATTGCCAGCTTAATGATTTCATAAAACACAAATAATAATGTATTTACCTTATGCTAAAGGCCAACATAACACCCAGAAACTGTTTTGTGTGCTCTTTCACTTAAGGTGACCTTCCCAATTAAGCTGACACAATATTCAGTGGCAAAAGATCCAAGTGAATGATAACTATCTGTGTGGCTGGAGTTATCTGCCAGCAGGCAGCACAGACAGACAGTTCTGAGCAGGGAAGGGTGGAATTATCTCTGTGTGATAAACAGGGCTGGGCAAAAGGAGCAGGGCACATAGATCAATGGCAGCAGTCAGACACATATTCCAGGGACTGTCACAAATATATATCTTCAGTAAAGCTGAGGGCACACGGACCGTTTGCTCCACTGCTCTCATCCTTTGATTTTTTTTACAGGCTGAGAGAAGCAGATCCGCTGACAGCAGTGGACCAAACTCTCCATGTGCCCTTAGCCTTAAGCTGGCCATACACGCACCGATAGTATTGTACAAAACCTCGTGCATGTATGGCGGATTGACTATACGACCGGTATTGTAAAGGCTGTGGATATAGGCCGTCTCATCGGTCAGGCAGGTTAAAATCTACGTTAAGGGCTGAATTGTTTCTACATCAGTGGAAAAAAGGTTGACTCGTTCTGCAAGCACTGACAGGCATTTCAATATACTGTGCACACAAGCGGCTGATTTTTGGGCAATAATGCTACTTTTGTGTTTCCGTACCAATGCTGTGTGTTGGTTTATACACAAGACAGGCAGACAGGAGTGGATCGGCCTTCTCTGCTTGAATTGTCACACCCCCATTTCAGCTGCTACTGAACTCCACCATCCTACTGGCTAAGGTTCCTGGGAGTAGTTTGCAACAGCTAGAAACAGGGTCGATATCAGTGATGGGGGGGTCACAGCCGGTACTGCAAGAATACAGGGTGTGAAACAAAGTAAGAAACGGCAAGATCCTGACTGTCAGCAATGGCAGCTTATAGCACGTGTATTATCCACGATTCCTCTTGAACGGGTCTATCCCAGTGTCTTTATCTGCCACCCCAGGAACATTTCAGTGCAGGGAGAATCGCTCTGCCCGTGCCTGACAGTCACTGATAACTTGTTTTGGGTGGAGGCCCCACAGGAGCATTAACTGTACCATTAAAGGAACCCAACCCCCAGAAACAAGCTACACCCAACTTTTTAAACTCTCTTCGTTTTTAAAAGAAGAACTAAAACATAACAAAGAATAGACTACAAAGGCTACATATTCAATGAAAGATGCAGCATACAAAAAAGTGCAGCGATCTGCCATCCTGTACTGAGTAACTTTCACAGACCTCTGCACTTATTTTCCTTTTTACACATGCAAGTTAGTGAGCATCAGTAGGCCCAGCCCTATCCTTACTGCCTGCATTAAGGTGGTCGCTGTAGAAAGCAACAGACCCTGGGGGCAAGGTTTTGCACCCCTTTGTGGTATTCAATGTGCACCCTGTTTCAGTAAATAAGCCCCATTATGTTTTGGGGTTCTGTACCAGTCCAAGAAAACCACAGCTATTATAAGTGAAGATCTGTACCATCGAAGATGACCCCATCTTCTTTTCTGCTAATTCTGCATCAGAATATAACTAGCCCTGTTGCATCAGCTTCTATACAGACAAATTTCATTTTCTGCTTGATGATTTCCCACACCCCCTAATCTTAACTTTCCAACAGCTCCCCAGGAACAATGAGCATGGCAGTGCCACTGACACTCCTAACATGATCCAAGATGGTGAACTCCTGCGCACAATTTTTAAGGTTTTATAGCTGTTATAGAGATGCTGAACCATTAGGCTGGTGCAGTAAGTTCCATACATAAATATAGCATTTCTAGCAATATAAATTTTTTGGGTTTTATTCTCCTTTAAAGGAGAACTATACCCTCTGGCACAAAAAGCACCCATAACTATCACTGCCAAGACCTCCCTCACCTCTCCCTTATGACATTGTTTTATTTCCTAAGACTAATGGCACCAATTTGGATTTTGTCACCTGCTGATTAAGGTCTGACGTCCCATGGAGCGTGTTTGTCACCCGTGATAGATCTCTGCTATCACAAGCGACTAACCGCTCGAAGTTGCCTGCAGGAGGAAACTTTGCTCGACTTCTGAAAACCAAAGCGATGCAAAGGCCAGTGGACAGGCACTTCAGGAGTGGTTAGTCACCCACGATTGCAGAGATCTATCACAGGCAACTAACTTGCTCTGTGGGGCATTAGCCTTAAGCTTTCTGCCTCCTGTATGGATGTGTGCTGACACATGGGATCAGCCTGTCAGTGCAAAAACAAACAAGGAGCAGGAGTTAGCAGACTGGCTGTTTTGTGTATATGCCGGCTGAAAAAATTACCCATTGGCCCTTAGCCCAAGGCTCACAGCACATGAGCTATAGACTCTATCAGCATTTGTATTGTGAATGCAGCCATGGGCTTCTTTTTCCCAAAATTCAATTAACACAAATGACAGTCTGATTTGTCAGGTTCAATCCCACTTCTACCCCAGCAAATAAATACCCATGCAGATCACATTTGGTGTGCCAACAGTCTAAAGGTGGTCATACACAGGCCAATTCTACCTGCCGATATCGTTCCTTTAGACCAATTCGTCAGCTTATCTGCCCGTGTATGGGCACAAACGATAGGCCTGCCTGACCAAGATCTGGCCTGAAATCAGTCAGATCTCAATCGGGCAGGTTTGGCCCCAGGGATAAATGACCGAATTAGCCCGATATCGCCTACACTTAAGTGGGAATTTCGGGAGAAGATCTGCTGGCTTGGTGACCTTGCAAAGAGAGCAGATCTTAGCGTGTATGGCCACCTTAACTCTAAGACTTATTCCAGATGGGCTGATACTCTGCAGGCAAGAATCAACACCTTTACTGGGATCAGCCTTTCCCTGTGCCTGCACCCAGACCCATTGCTTCAGTCCAGGTGCAGGCACACAGAGCGAATTTCAGCACTGTGTGAATATGCATTTCAGAGTGAAAATCTGCTCTGTGTGCCTGCACAGGGAAAGGCTGATGCCAGTGTAAGGTCTGATTCAGCCCTGTGTGGCATTAACCAAAGAATATAACTGTTATGTTGCTCCCTGCACACATATGTTAGGTATGCTGGAAATTGTAGGCAACATTTGGGTGCTGTATGTGTTACATCCCATGCAATGCTGGAGTCAAAAAAGCTATTCAGGTATCCATCCCAGTGTAGAAAATGGCACTTTAAAATGTGCAGCTGCTGCAGGGCATCTTGTGAGCAAATCAGTCAATAGATGCTCGACCACAAGAGGTTTAACTAGAGACGGACACTTCTGATTCCCCAGAGGATTTGGGAAAGCTTTTAGCACCACTGAATGAGGGGATTAGTGACCCTCCCCCTAACACCCCCCCACCACATGCATTTACAGCCAAAGCCAGGACCTGCTAACAGTACAAATCAAAGGGGAACTAAACTGGTTCAGCAATGTCCAACCTGTTGTTTAACTAGAACCCAGCTGCCTGATAGTGGGGTTGGGAGCTGCAGTTTAACAACAGCCCGAGAAGGATGGACATCTTTGATATAGATACTGAAAACAACTGTTAGGGTAATGTTGCCCACAATAGATCTCTGCTAACACGGGCGACTAATCTCTTCAAAATGCCTTTTTCACCATCATCAAAGGGAATCGGTAGTAGAAAGGCCTATCCCTTTAGTGCAAAGTTGCCTGCAGGGGGAAACTTCACCATTGATCAGCACATACCTCTTTTACCACTGAACCCCTAGTTTAGGCCCTTTTGTTATTCCAGCACTCCAAATCCACCCCCCCCCCCATTGCCTGGGTGAATTCCAGAAAAAGTTGAGGGCCCCTGGGCAAGCCACCCAGTATGGAGGCATCAATACACAGACAAGCAAGTTTGCCCCCCCCCGTTATCTCTTTCAAGTGCATGGCCCCAATTCACCACCCCCTTAGGAGAGCTCTGCCCTTGTAGGTATTCCGCTGATGGCAAATAAAGAATTGCATTGACTGCAGGTCTCTCCAGCAGTGCATGGGTTAATCAGCAAGGCAGCTGCTGTGTCTGGTTCCAACTAATCCTGGCTCTGCAAGCCTATAGCAATACACACAGAACTGAGCAGCTGCCCCTTTAAATGCCATTTCCTTCTATTGTATTTGAATGGTGATCAGGAAAAAGGAAATGAAAGCAGAGTCAGAGGGGCTGAGCTTAAAGGCTGCAAGAGGGACACCAATCGGCTATATAAGTGACAGTGACACAGGGCTCCCTCCCAGACCTGCCTGCAGCAG | ||||
Promo 12 | p2 pax3 | 2916bp | 109,5 | Xenopus Laevis | Neural ectoderm | Unpublished results | GGCACTTAGGTTGGGAAACATTCCTCACAGCCCTGGGCTCATCACGTTACCTCCGAATTTCTACTATTAAGAACACAAATACAAGGTAATGGGATTCAGACTATGGAAAGTGAGCGCCATGCTGCTCAGTCATCCAAAAGAAACCAACTAGTTGTCCAACCTGAATAATACGCGTAATGCGCCCTTGTATTTCTCATTATTGTTAGTATTGTGAACCTCCAATGACAGTGGCAACTGGCAATTTAAAGGGACTAAAATGCTTATATAGTATCTGTCGGATTTTGTTGAATATATGTGTACTAAACAAAACCCACCCCCCAAAAATATTCTGATTTCTTTTTCAAAGGTAGTTTCCATGGGGGAAAAAATAATTTTCAAAATAACTAGAACATAACTTAATTCTTCCTTTTCAGTGTGATGTAATAGTTTATTATTCATAGTATATATCAATATATTAAACATAAATCGTGCAGGGGGTGAAGCAGGAGAGTGGGGGTTGCTGGAAGTTGTATTTTAGCCCCCCTTTTGGGAGAACTGTCAGCTTGCTGGGACTCACAACGGGAGATGGGAAATATTGAGTCCGTGACTAAACTGATCTCATGGTTTATCTCATAAAAATCCCGTTGTACTCTAAAAACTGGACTTGCTGAGAGGTTTGTATCCCCTGCTCTGCATTGGAAGAGAACTGAAACCAACTGGGCACCATGGACTCAGCACAAGAAACTTCATCATAGGGCATTGCTCCCAGATTCCATGAAAATACACTAAGTACCTTAGTGGTGTAACTTGTGGCACAAGGAGAATACGTTGAAATCTTGAGATTTATGAGAGGAGGGAGAGATTGACTACTGGGCGACACGTCTCCATTCTGTAGGGAGCAGTTTTAAGCTTAGTCCCCAATTCTCCATGCCCTGTCACCACCCTCACACATCTATCATTTCCAGCTACTATTTGCTTTTCCGTAGCTTTTTAAACGAAACTTTCACACGAGGGGGCCGCTTGGCACTGGATACACTTACATGGGTGAGAATTATTTGCTGCAACTCAGTGCGCCTCAAACTAAAACACTGGGATCCTACAACATAGTCCAGTAACTGGAGGCTGAAAACACCAATTGCAATAATATTGATATTAAAAACAACGACAGCAATACTACTAATCATTTAATCAACAGCAACAATGATATCAATAACAAGAAGAAGAATATCAGTAGCAGTAATATAATGAAGGACAATGTCAATTCTGTATTCTTATTCTCATTCCAGTGATGAGGTGAATATAAAATGAGTACAGGACAAGGCAGGGAAAACGGCTACACGTGGATTTACTGCAAAGTGACTTATTTGATCCAAATAATAGAGCAACGAATGCAGCGCAAAGTGCGCAATCCCCGGGCGGGTAAAACTCCGCGCAAAATGAGCAGCGCGATCCTGGGTGATATTTAATTTGATATTCCCTGTACTCAGCAGTAAAAGTCAAAATCCAGAAGCACACCAAAAAATAACTGCTCCGTGAAAATAGGTGATTTATTTATCCCATAAGCATTTCACAAACAAGGCAACGTTTCGGGCCCTTTATCAAGCCTGTAGCCAGCAGTAACACATAGGCTTCCCAAACTAGGATAATGAAGAAAACGGAGGGGGAATCCCTGGCCCTAGCCGCATTGTAGATGTGACAAGGGGAGGAAAAATTGTGATTCAAATGGGGGGGTTGTTTGTGTTTTTCCCCTCTCTTGATTTTGTAGAGGGAACAGCGGGATTTGACCCCTTGAGTGCCGGATAAGGCGCAGCTCTGCAAGTAGCGGGGGTCTCCGTGCCCTGCCTAAAGGGTTAAAGACAGAAGTCATTGTTTCTTTTATTTCAAAGGCCAAAGCTGCCTGTGTCCCATCCCTTTGATTGATGAAGGCAGAGAATGAGTTCAGTGGCAGGAGTGACAGTCACTTGAATGGCCCATTGCAAATAAAGGGATCTGCTCCAGTGTATCTGCTGCCTGGCATTAACCATGTTATAAATAAATGGGGTCGGACAATGGCACCTTATGGAAGGGAGAGACAGGCTTTATAGCCTTCCTGGCGTCATCTCCTGCTGGGGATCCTCCATCCACTGCTTCAACTGCTCTCCCTCTATTTATCCAGCTCTCTGGCCACTGCTATAAATTCCTTTCAACAAATGCTGCCCCCAGTCCGCCCACTCTAAGCTGGGAATCACACAAGTGCGCCTGGCTGCTACATTACTGTGTGTGTGTGTGTGTGTGTGTATGTGTGTGTGTGTGTGTGTGTATGTGTAGGGAGAGAATTGGGGTAGGGGAGGGGAGAATAAATAAAATATAAATAAAATATGGAGGCATATTTACAAAGTGTTCCCTGCTCTGTGGCAATCCTGTATTTGCAAGGATTTATGCAGAAAAAAAGTGTCAACGTCAACCCCAACAGGGATTGGAATCAATGGGTTAAAAAGACTGGCATCCCCCAAGTAAAACTGCACTTTAAATCCATGCCCTGTACTGCTATGCTGAGTGGAGACACTTGATGATGATGATGATGATGATGAAGGGGAGGAGTTAGCAAGTCCCCTCTCTGGGATGGCAGGGATTCTGACCAATTAGAATTGACTTCCCTAGACGGGACAGCAGTCCGATTGGCCAATAGCAGAGGAGAAGTCCTAGTGCAGCCTCAGCTGAAAGAACTAATAAATACTTCCAGGCAGACTCGCACCTCACTCTCAGCAAACAGTCAGGAACTCTCTGGGTCCACACGGGCCCCCGGCAATTCCCCCTCACACAGTGTCACTAGTTACCCTCCGAGGCAGGACGAGGCTCTGCTACCTTCTCTGGCATCTCAGCACAGGGTACCACCGACTCTCACTCTCTCTTTACAGGGGGACTTGGATTTAGTTCTTTGTAAGGGAACTGATGATG | |
Promo 13 | p2 vimentin | 7205bp | 187,4 | Xenopus Tropicalis | Neural progenitor/glia | Love et al., 2011 | AATTTATTGAAAAATATGAATTTTTTTAGAACCTTTCAGAAAGTTTCATACAGTATTCTGCAAAATATGTCCCTGCCTTCATGTTTTTCCTGTATATCCACAAATGTACAAAATATACAATAATTTTGGTCTTTTTTATAGGTCATGACTCGTCTACCATCAGTTTAATACATCCATATTAAGAGAATCTTAAAATTTCCTCAAACTCTCAAACCCTGGTTTCTCTTCTCTTGGTAAATCCATGACTCCCTATTATAGAAAATATTGTAGATTATTTAGTGTATTAACAATGTGCCTGTGCCTCTTGTGCGTCTGTGGACAAAAAGAATGAAAGATTGCAAATACAGGTATGGGACCTTTTATACAGAATACTTAGGACCTAGGATCTTTTGGATAGAGGATCTTTCCATAATTTAGAACACCATACTTTCAGTCTGCTAAAAATCATTTAAACAATAAATAAACCCAATAGGATTGTTTGGCCTCCAATAAGGATTAATTAGATCTTGGTTTGGATCAAGTGCAAGGTGCTGTTTCATTATTACATTTAATTTTTAAACATTTGATTTGCTTGATTAAAGTGTAGTCTATGGGAGATGGCCTTCCCATAATTCTGAGCTTTCTGGATAACAGGTTTTTAGATGAGGAATCCTATACCTACTGTACACTTGGTCAACTTTTGTCAAAGATTGAGACCATGCTAAGATATATTCATAGTGTGAGATATAAGGACTAATTTATGACTGCACAGAGCAGTTGCAGACACAAGTTTTCCAAAGTCAATTATGTGAAAAAGATTTGTGTTGACATGCACGCAATATAGTGGGCATTTTAATGTATTGGCAGCAAATGCTGCACTGAGGGTAAAAAAGCATGGCAAAATGGCATTTTGCACACTGTACAATGCTCTAGTTTTTGAACATAAATGAGCCCAATATTGTATGGATGCACCATTATTACTATGCATGCATTACTACATCAAGAAGTTAGTAAATATGTTGAAAAGTTCCGACTTGCTATTCAAAATGGAGTAGAAATTAAAGGGGCAGTTTACCTCCTTGTTCAACATGAGAGCAATGAACATCATTTTTGCCTATTGTGTCACAAACAGGGAGACTGAAGCTACCTCCTTGTGAAATAGATAATTAGTATACAGCATACTGATAATCCCCCTGCACAATCAACTGTTTCTAACAAAACAATCACAACAAAGGCCAAAGGGAGGGGGTTGTACATTGGTGATGCAACATAGCCTTTCTAATAAGAATGATTTCACAAACCAGGATTGGACTGGTTTGCCAGAGGATCTCCCAGTGGCCCAGCCAAGTACAATTCCCATCACCCCTTTCTTATTTCCACTATAGCTCTGTATGACATCTACATTTAGTACTGTATATAGCTAATTGTTGCAAGAGCGGGCCCTCCATGTCAGATTCTCTAACACTGACAAACACTGCTTTAGAGTAGCTGCTTTGCATTTGTGTGGGGGAAAACCAGAGCAATAAAATAGCAATATAATAGTTATGCTCTTTGATGTTAATTTAAAGTAGAGGAATAACAAATAATTGTTGTCTCCTAACAAGGCACATGGTCCACATATTTTAGTTTGCTTTCCATATTAATATTCCCGTATATGGAAGGCATTTCTCTCAGTCTATTTTAAGAACTTGCTTTTATTGGAAAGGAAATCTTTTATTTTTATAATTCAGCACGTTTGCACAAAGAAAAGTAGAGCAGGTTTGCTAAATAACAAATTTAGACAGAAAGACTACACATACAAAAAAAATAGATATCAAATTTTATTAAGTGTAAGTATTGGTATAATACTGAGAAAGGCTATTTTATACATAATAACAATCAGTTACAGGTAACCTGATCCGGAAACCCATTATCCAGAAAGTTCCGAATTATGGAAAGACCATCTTTTTAAAAATGATTTCCTTTTTCTCTATAATAATAAACCTTGTAATAAAAAACCTTTATAATAATAAACCTTGTACTTGATCCCAAGTAAGATATAATTAATCCTTATTGGGGCAAATCATTCCTATTGGGTTTAATTACTGTTTAAATACTGTTTAAATACTGTTGGGTTTAATTACTGTTTAAATACTGTTTTTGCAAACTTTAGGTAGGGGATGTGAATTACAGAAAGACCCCTTATCCGGAAAACCCCAGGTCCCAAGCATTCTGGATAACGGGTCCCATACCTGTACTAACGCTACGTATGTTGTCTTGTAAGGGGAAATTAAATATTTCTTTAAATATTATATTTCTGGGACATGTTGAGCTATTGTTATGCCATTTGTTTTGGCCATTTCTGATGAGGCCATTTCTGATGAGGCCATTTCTTGTTGCTATATGCAACAATCATAAAGTGAGTTTAAAAGAGACATCACAACTAATAAAAAAAAAAAACAAGCTTGTAGGCAATAATGAATAATATATGGTGCTGGTTTCAATCTGGGCTAAAAATTATCATTATCTCCCTATAGATCTGCTATGATCCATGTCCATGTTTCAAATGAGAGGTGGGTGTGACCTAATGGTCCCTGCCAGAGGCAGACTATGGGGGAGACAGCCAATTACAGCCCAGCAGTCACATACGCAAACACGGGCTTCAGTTCCCTGTCAGGTAGATTGGTTCCTATTCTACTGTGCAGTGGGCTGAGTGCCACCAGCTGTATATGTATATGTACTAATAATCTGAATTAGTTACTAAGCAGCCTTATATCGTGACATTTATATTATATATATATACAGTATATTGTGAGTGGGCCCCTAATCAGCAGAAAATAAGATGGGGAGCTACTAGGGCATCTTTAGAGGAGCAGATCTTCCCTGCTAAAGGGCTGTGGGTGCCTTGGGCTGGTACAGAAGCACAAAACATAATCTGCAACATTTCTAGCCTACTTCTTTAGTTAAAGTTTAGTTCTCTTTTAAGGGCTTGGAATAAAGCAATTTAGCAATCATTATAGTAAATTAGCCCATATGCAAGAATGCTGTATTATAAACATAGGTAGAATCTCAGCCATAAAGCAAGACAGAACTGCTGATTTGTGATTGAAAAGAAGGTGATTGAAGGGTTTGACTGCACTTCCATATGCAAGCACAAACATTTCCAGAATTTTAAGGCAGTGTTTTACTAGTTCCCCTGTTTCCAGGAGCAAATAAAGTGTATCTTTTAACAATACTGTCCCTTTAACGATAATAAAATAGAAGTAATTGGCCTTGGCAAAATGGCAGATGCATGTCTTAGAAGCAGGATAGATAAAAACCATCTACCATACTGGCTTATAGCTCTGGATACAGACAGACCCTGCATGAATAATTCTAGGAGGAAATTGTCTTCTTTTCTTTCGTTGTGTTTTTGGAAAGGATCCCAAAATTTCCCGCGCTGTGGTCACGGAGGAAATGTCTCACAAGAGAAGGGAGGGCCCTTACTGTGCGCAACAGACACTCATCCCTCTCCTCTCCCTCCTCTCTGTCAGCCAGATACTGGTGTAACTGCACAGGGAACCCTAGTGCTTCCCTAGTGCCACTGAGCCTGCAACTTGCAATGGGGGACTCTGGACCACCCCAGGGCTCTCTGATTTAATAAACTGGGATACATGTTAAGAACATATGTTTAGGATATGTGCATCACGTTGGCTCATTCACACGCCTTCCTAAAAGGTGTATTCTTTTATTTCCTGTTATCTGTGGTTTTGCTAAGAAAAGCATGTGTGCAGCCCTTCACGCTGCCTGGCATAAATGCTGTAAATGCAGAATTGCAGATACTTTCACAAATCTGGTATGTTTCATAACTTACATTTAAATTGAAGGTAGAAAACGAGGGGCCTCATTTTATGTAAGAAGGCTGAGTTGGATTGCCATAAATCAAGGATGCCTAGGTGTTGTTGAACTGCAGCACAGCTTTACTTCTGATAAAGTGCCACTGTGAACAAAAATGATTTGCAGCCCTAAGGGTGAAGACACACAGAGCTACTTGTAGCAGCTACTTTTTCACGGTTACTGAACGCCAGAAAATCCCCTGCCATAGACAATATTGAGAATTGCCTCTGCTAAAACACATGTTGAGACAATTATCAGTAAATGATCAGCATTGTTTATTTTAGTAGCCACAACAAGTAGCTGCTACTAGTAGCTCTGTGTGTCTTCACCCTAAGGGCAGTGGTACACGGGGAGATTTGTCGCAAGCGACTAATGTCCCCACATGCCATCCCACCGGCTTGAATGTAAATCACTGGTGGGATGGCATACGCTTTGCTGCAATGTCCCCAAGTTTCCTCTTCAGACAACTTTGAGCGACTGTGGGACATCGCAGCGACGTGTATGTCATCCCACCGGTGATTTACATTCTAGCCGGTGGGATGGCATTGCGGGGAGATTAGTTGCCTGCAACTAATCTCTCCATGTACCACTGCCCTAAGAGTGCTCAACTGCAACTATCGGCCTAACTCTCAAACTTTATTGTACTATATTTCCCATCCTCCCCTAATAACCAGTATGGGATCTATAGTTTAGGGTAGCTCAAGTTCTCAAGGGACCCAGTGGCCTTATATACAGACACTCACAGCTATAAGCAGCAGGAACAGGAAATATATTGTAATCAGCTCATCACCATGGTTATTGCCTGAAAATACATCCATCCATCTGGGGTTGCTTATGACTTCATTCTACCCCCTCCCTTCCCCCCAGCATCATGCAGAGGAAAGGGGGAGGGGGTGGTGGCTGGGACAAAATGATGAGAGGACAAAGGCTGAGCACCATAAAATATGCAATGGGTTTTGTTAAATGTAGATGAACTGCAGCCCTCTGCTTCATGTTCAACTACCACTCTAACACTAGAGAGTTACAGTTTAAAACCTAAATCTTGGGAAACTCACACTGGACATCTGAGGCAATAAGTTTACATTGAGCCAAAGGCTGCATAAAGAAAGTGTATCATTGTGCTGTGTCCCTTGCTTTGGGGACACGTGTTAGATGTGAGGGATAAGAAGGACAGAATAGCGCAGAGGACCAACGCTCTTCTGCAAGAGATATGAATTAACTGAGCTCAAAGTAGCAGATCGCTAATCCAGCTTGCTCATTTCTATTGGCTAAGAACCTACAAGGGGACCCAAGCAGCAAATCCTAGAAATTCAAGTGTCCAAGGAAGTAAAAAGAAACTCAGAGCCTAAGTACCATCATGGTAACAACCAAGTCATCTTACAGAAGGATTTCTGAGAGAAACCCTAGGTCAAGTTCATCTGGAAGTTGCTATGTGACCTCCAGCACCGGGTATTCCATGGGCAGTGCCACGCGCCAGGCACAGGCAGCAGCAGAATGGCCTATTCCACATCTGCCTCCCCCTCTGTGTTCAAGTCCTATGAGGAGCCGAGAAGCAGCCTTCCCCCAGCCAGCCTGGGCGACACCATGGGGCAGAAAAGAGGATGGGGAGCTACTGGGGGCACATTTGGAGCTGTGATTTCTTTGGGCTGGTACAGAAACCCAAATATAATGTACAACATTTCTACCCTGCTTATTTAGTTAGGCTTTACTTCTCCTTTAAAGGATACACTGTATTTGCACCTGGGAACAATGCACAATTTTTGGTAATGGGCTAGCGGATAAAATTACAACAGCTAAGAGGCCTTTAATAAAGGTCCCTGATGCTAATGGGCAGGTCAGCAATTTTCCAAGTTGCACCATAACCCACAGATTGCCACCCTGCATCTAAGTTCTCCTCCATTTTTTTAAGACTAAACCCATTTCCAATAATGTTGTGGATGGGTTGAGTTGGTGCAGTAACATCACCAGAGGAGTGACCAGTGGACAGAGTCCCTGTTTTAAATTTTGGCTACATAATGAACAGCACTTAAGGCTCAGTTTCTCAATCCCCCAACTCACTGATCTGCCCATGGCTAATCCTGGGATAGCAGTATATTTACTACATCTAAAAATGTGATTGTTTTCTCAATTTGATTACATGTTCTTGGAATGACCGACCATACGTTTTGCCTATCTGTGGAAAAATCCTAATCTACAAGTATACAGAAGGATAATAAAGCCAAGCACCAAAAATGTGTAATTATAACTAATTACACACCTGTCCCATTGGCTGAAAAGATCTCAGATGTATCATCATACTAATTCCTTATGAACTGCTGTGCCACTAGGGGGATTTCAGCACATGTTGTACAGGGCAAGATCTAAGTACAGTATGTACCAACAGTATGAAGTATTATTGCTTCTCATACCTGTCTAAGTGACTGAAGGTTGCCTTCTGCTTCTTCCCTCTGGACCATTTCATCTTGTAGCCTAAAGCAAGATTTTCAGCTCAAAATGAGAAAGATTTGCAAACCTGGGGTAAAGATTTAATTGTAGGCTTTGTAGAGCTAACAAAATAATTTTTTTTATTTAGCACTAAGCAAACTTTGTAGGACTTTCCGCTGATTGCTTTAGCAGGGAATGGGGGATAATTTTTGGGTGATATTGTACCATCTTCTAAGCAGCTTAAAAAAGAATTGTCTACAGAGCAAATCTGTCGTTCAGCTTTTGAATTTCAGCCTTTTCGTTGGACTTCATTCTACCCCCTCCCTTCCCCCCATCATCATGCAGAGGCAGAGGGAAGGAGGAGGGGGTGGTGGCTGGGACAAAATGATGAGAGGAGAAAGGCTGAGGGTTGTGAGCACCATATAACATGCAATGGATTTTATTAAATGTAACGGAACTGCAGCCCTCTGGCTCTTGTTCATCTACCACTCCAACACTAGGTTGAGAGTTACAGTTTAAAAAAGCTCATGCTGGACACCTGAGCCAATAAGTTTTCAATGAGCAGAAGGCTGCATAAAGAAATTGTATTATTGTGCTGTGTCCTTTCTTTTGGAGCCACACATTAGATGTGAGGGATAAGAGGGACAGAATAGTGCTAAGAGAACCCAGCCCTCTACTCCTGCAAGAGATACGAAGTAACTTTCTGAGAAGATGAGCACAAAGTAGCGGATCGCTAGTCCAGCCCTGTGTGTTTCTATTGGCTAAGAACTTACAAGGGGAAACTGAGCAGCAAATCTGATAGGTTGTCTGAGGCATCTGTCATGGGAAACCCTGTTAGCTTCTGTATAAGTACCGCCCTTGGTGCCAGCTCTCTGTACTGCTCAAGTACTGGCTGGGTGAGGAGAGGGGTGCTCACATAGAAACTCAAGTGTCCAAGGAATTACAAA | |
Promo 14 | p2 Brachyury | 3856bp | 103,6 | AGATGGTGTATTAGGAGTCTGCAATGGATGCCTCTCTGGACCCAATATAAACCACTATGCAACTGCTCTGCATTTCTAATTTGTGTTTCTTTCCTAAGTATTGCCTTACATCCTTTGCCATTCTAAACCCATATACTGAGAGACCCAAGCAACCAGGAAGCAGTTTAAATACAAAGCTACAGAACAAAAAATGTGAAAAATTCAAAAACCATGAAAAACGAGCAACAAACATAGTGCATCAGAATGCCATTGTCTTGCCATAATCGTTTTGAGAATAGCTTCCCCACTGTTCACCTAATTTGGATAAATGAAAATAATATAGCATACATATCTATATATGTAGTGGGCACTCAATCATTCTTTTCCACAAATATAGTTTCTTTTTTCTTATAGGTTTAGAGTTATAATGGTTTAATAGTGTACATCCTTATCCCATCATTTTAACTCCTGCAAACCACTTAACCATGCTATTTGCCAGTACTGCACTGTGGGTTGCTGGAATCAGCCACTTAGAGTAGGTGATATCTGGCATTTTAACGAATTATTACAAATATTGATGGTTCTCCCTCAATATAACACTATATAGTTCTGTTTGCTTCAAAACTCATATGCTAATGTAGCCCCATATTGTATTACTGTAATACTGAATACACTGCAAAAATGGATAGGAAATAATGAACCTCAGGGAAGGCAAAAAGCTGCTTCTGGTAAGTTTAAGAGAGCACTATTGCGGGCCCCCTCTAGACCCAGTCTAACGCTAAATTTGTAACCACAGAGTGGGAGAGAGGGGGCCTCAGGCGACAGCAGCCCCAGAATCGCCGCTGAAAGTATGACAATATTAGAAATCACCATATTAGGGGTAATTTACAAAATCCCCTGCTGCAAGTGCTAATACATTAAAGGAACAGTAACACTAAAAAATGAAAGAGCTTTAAAGTAATAAAAATATAATGCACTGTTGCCCTGCACTGGTAAAACTGGTGTGTTTGCTACAGTAACACTACTATAATTTATATAATAAGCTGCTGTGTAGCCACGGGGGCAGCCATTCAAGCTGGAAAAAAGGAGAAAAGGCACAGGTTACATAGCAGATAACAGATAAGTTCTGTAGAATACAATAGTGTTTTATCTGTTATCTGCTATGTGCCTGTGCCTTTTCTCCTGTGAATGGCTGCCTCCATGGCTACATAGCAGCTTATTTATATAAATTATAGTAGACTTTCTGAAGTAAACACACAACTTTTACCAGTGCAGGGCAGCAGCACATTATATTTTAGTTAGTTTTATACACTTTAATTTTTTGGTGTTACTGTCCCTTTAAGGAAAGTAGGAACCCCCTTAAAGCTCTTTACAGAGCACTTGCAGCAGGGGATTTGAGTTAGGTCTACCTTGCACCTCAATAAAATCCTGCTGGCACTGCCTACTTGCACATCTCAATTAAGGGCATGAGTTGTGGAACAACTTACAAAATATTGTGCCGGGTACAGAAAATATTCAGTGTAACTGCTTATTACCGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCAGTTAACCATCTAATCATAAGACTAATATGATACACAGGTTAATTAACCTTTTACTGAACAATGCGACTATTACAATGTAAATGTAATAATTATCCTACAATCCTCCATGTATTGCTAAAGTTCATCTCCCAAAAGCATGTGATAGCTAAAGGCTGACAGGGGCTGAAGGAAGCTATAGTTAAACTACTGCTAGTTGCAAAATGGCATACCTGTGTAAAAGCATAAGTTCCGGATCAGAAGTTCCCTAAACCTAACACAGATACTTTTTGTTTTCCAGAATCGCCAGTTGTGTATTTTAATTAATGTACACCTGAATATGATATTGTAAATTACCAGTATCCACCAATCTCCCTTTAATCTACTTTGGGGGGGGGGGGGGGCATTATGCCAGCTGAATTTCAAATACTACAATGCACAAGTGATCTCTCCTAGTATGAAAAGTATGTGCTTCTTATAGTACTTTTAAAAGCTGTGGAAAGAAGTAGCCAGTTCGTGGCAATGCAGATAAATCAACTGAAATTTTATTTAGCATTACCCAGAATGCTGTACCCCAGATCAACAGTCTCCATTTCATACTAGTTATGAATATTATGATTACTGAACATGGTTTACATTTCCTTCCTCCTATACGGATGGAAGCAGTTTCACTTATAGGTTTTTCCACTACATTGCTTTTTATCTTCCTCTGTGTAAAGAGGCTGGGAATGTTTAAAGAGATCCCACTGAACTGTTTCCTGCCCATTGTCACCTTTCGCCTTCTAACCACAGATTCTCACATTAATGTCCCACCCCCACAGCACATAAGGCACATACCTTCCTTCACACACTTATTATGAGACATTGAGGATTAAGAGAATTTTACTGCAGGATTAGTTTAAATAACGAAGGCAGAAATAAAGAAGGCAGATTACAAACAATAAAGACATGTAAATAAATAACAATAAGTCAGCCCAAGTTTGTTTGGTCTGCATTTTTGGTGGCAAAATGAACGCATGTGTGTAGCAATAGCTGTCACGATTTATGTGAGCAAAACAGCAAGTTTCATCATAGTTTTTGTCAATTTTTTTTTTTTTTGCAGCTGCTTTTGGTCATCTCCATATTCTGCTGCAAATCATGCAGGTTTTTGTTTATTAGAAATGTATCAGCGTATAATTAAATGTAATTATGGGCAAACAAAATACTTATCATATACTGCATTTACTAATAATGTATTTATAAGTATTTAGTCATGCTAAAAGAATAGTCACCTGTATCACTATACAGTTTTATTGTGTAATTTCATATAATGCCACAGAATACATTTTGATGTTAGACTTTAGTAAACAATAAGAAACAGCATACACAGCGTCCTAGTGAATATGTGCGCAGCAGCTTCAGAAGTGCTTGCAGTTTAGTAAAATGGTGCAATTTATTATCACCTTCTATTGGTGAAGAAACCCATGTAAATATGATAGGCAAAGGTATGATGTAATTCAGTCTCCATTCATTTAAATGCACTTTTTGACAATAAAAAACATGTTACCCATCATTTTAATATGCAACAATAGTAATTTAAGCCTCAAAACCTTATTTTTATGCAACATCTGCCATTGTATAGTTTGAGCAACCTATCTAGGCCAGCTAAAATATAGAATGATAAAGTGACCAGGTGTCAGTTCCTACTGGCTGTAAGTTAATTGAAGGCAGGTTATAGGGGGTGACACACCACTTCCTCACTGCAGCGCTTTGATATAGGTGTAAACATTTGGGTATTTACATAAAGTACCTCTGACTTGCAATGAAGTTCCCAGGATTATCATAGGGCTCAGCGCTGGGTTCCCTTTGCTCCAATCAGAGCTTCCCTCACCAAGATCAAAGAGAAATCTGGCCAATGAGCTGCCTTGCCGCACTAAGGGTAAAGGCACCATGGCTCGTCAAGGCCTATTTATGGGAGAGAGAAAGATCACTCTCCTAATCAAGGACTCTTATTC | ||||
Promo 15 | p2 CMG long | 92,4 | Sequence | |||||
Promo 16 | p2 LMO2 short | 2007bp | 88,3 | GGATCAGCCTGTCAGTGCAAAAACAAACAAGGAGCAGGAGTTAGCAGACTGGCTGTTTTGTGTATATGCCGGCTGAAAAAATTACCCATTGGCCCTTAGCCCAAGGCTCACAGCACATGAGCTATAGACTCTATCAGCATTTGTATTGTGAATGCAGCCATGGGCTTCTTTTTCCCAAAATTCAATTAACACAAATGACAGTCTGATTTGTCAGGTTCAATCCCACTTCTACCCCAGCAAATAAATACCCATGCAGATCACATTTGGTGTGCCAACAGTCTAAAGGTGGTCATACACAGGCCAATTCTACCTGCCGATATCGTTCCTTTAGACCAATTCGTCAGCTTATCTGCCCGTGTATGGGCACAAACGATAGGCCTGCCTGACCAAGATCTGGCCTGAAATCAGTCAGATCTCAATCGGGCAGGTTTGGCCCCAGGGATAAATGACCGAATTAGCCCGATATCGCCTACACTTAAGTGGGAATTTCGGGAGAAGATCTGCTGGCTTGGTGACCTTGCAAAGAGAGCAGATCTTAGCGTGTATGGCCACCTTAACTCTAAGACTTATTCCAGATGGGCTGATACTCTGCAGGCAAGAATCAACACCTTTACTGGGATCAGCCTTTCCCTGTGCCTGCACCCAGACCCATTGCTTCAGTCCAGGTGCAGGCACACAGAGCGAATTTCAGCACTGTGTGAATATGCATTTCAGAGTGAAAATCTGCTCTGTGTGCCTGCACAGGGAAAGGCTGATGCCAGTGTAAGGTCTGATTCAGCCCTGTGTGGCATTAACCAAAGAATATAACTGTTATGTTGCTCCCTGCACACATATGTTAGGTATGCTGGAAATTGTAGGCAACATTTGGGTGCTGTATGTGTTACATCCCATGCAATGCTGGAGTCAAAAAAGCTATTCAGGTATCCATCCCAGTGTAGAAAATGGCACTTTAAAATGTGCAGCTGCTGCAGGGCATCTTGTGAGCAAATCAGTCAATAGATGCTCGACCACAAGAGGTTTAACTAGAGACGGACACTTCTGATTCCCCAGAGGATTTGGGAAAGCTTTTAGCACCACTGAATGAGGGGATTAGTGACCCTCCCCCTAACACCCCCCCACCACATGCATTTACAGCCAAAGCCAGGACCTGCTAACAGTACAAATCAAAGGGGAACTAAACTGGTTCAGCAATGTCCAACCTGTTGTTTAACTAGAACCCAGCTGCCTGATAGTGGGGTTGGGAGCTGCAGTTTAACAACAGCCCGAGAAGGATGGACATCTTTGATATAGATACTGAAAACAACTGTTAGGGTAATGTTGCCCACAATAGATCTCTGCTAACACGGGCGACTAATCTCTTCAAAATGCCTTTTTCACCATCATCAAAGGGAATCGGTAGTAGAAAGGCCTATCCCTTTAGTGCAAAGTTGCCTGCAGGGGGAAACTTCACCATTGATCAGCACATACCTCTTTTACCACTGAACCCCTAGTTTAGGCCCTTTTGTTATTCCAGCACTCCAAATCCACCCCCCCCCCCATTGCCTGGGTGAATTCCAGAAAAAGTTGAGGGCCCCTGGGCAAGCCACCCAGTATGGAGGCATCAATACACAGACAAGCAAGTTTGCCCCCCCCCGTTATCTCTTTCAAGTGCATGGCCCCAATTCACCACCCCCTTAGGAGAGCTCTGCCCTTGTAGGTATTCCGCTGATGGCAAATAAAGAATTGCATTGACTGCAGGTCTCTCCAGCAGTGCATGGGTTAATCAGCAAGGCAGCTGCTGTGTCTGGTTCCAACTAATCCTGGCTCTGCAAGCCTATAGCAATACACACAGAACTGAGCAGCTGCCCCTTTAAATGCCATTTCCTTCTATTGTATTTGAATGGTGATCAGGAAAAAGGAAATGAAAGCAGAGTCAGAGGGGCTGAGCTTAAAGGCTGCAAGAGGGACACCAATCGGCTATATAAGTGACAGTGACACAGGGCTCCCTCCCAGACCTGCCTGCAGCAG | ||||
Promo 17 | p2 Ef1a | 528bp | 128,9 | AACACCATTGTTTTTTTAAATCAACATTTGATCAAATAAATCTGATCACGACAAAATTAAGCATTTGTTATTGTGATGAATATTATTTTTTCTAGAATTTAAGATAAAAAAGAAATGCAAAAGTTAATATTTATATTTAGAAATTATGAAGAAATGTTGTCAGACTTTCATAGGATGAAACAAAAATTAAATAAAATATGAATAAGTAACATACTTTATTCAAACCTACGAGTAAATCGAGAAATTTTCTCAAATAATATAGCCTACACTGACATTCATGTTAAGATGGTCATCTTCTCCCTACTGCAGATTTAGTAGATATCCATCAGTTCTGTTCATCTCTTATTACATCTGCAATATTAGTAAATCTACTGTATGACACTCAGCCAGATGATGGGCGAAACAAAGTGGTGACGTCAGCCTGACGAGGCGGGGAGATTTTCAGTCAATTGTGTGTGATTTGCGGGTCGATCAGGAAGCGGGGGGTTGCAGCCGGCCGCTCGGTCCTCCTCTCGAGTATAAATTCTC | ||||
Promo 18 | p2 Hsp70 | 487bp | 62,5 | Xenopus Laevis | Heat shock inductible | PrsI | Beck et al., 2003 | cccgtttagcaggaaatagccttgggttgatgggaaggaatggacatggtcagagacaagacagaagtacagaggaacgcatatctatttcacatgagaaaacgactctctcgagaaagctcgcgaatcttccgcgattgtgactaactcagcaaccgtgacactgccggaaacctcgcgaaagttcttcgggtgatctcatacgcaaaactggttgctgattggctaacgaaatggaagcctcgggaaacttcgggtcggttgctaaggtgaccgtggctggcgtggcgtgtactgtgccttgattggctaatctgttctcagagttctgtataaatacagcgggtttgaagcgatgtggaagtagcagaatctaagctgacacttgtgagatctgacagagaagagcaactgcacagcgatacttactgggcaaagacgcagctgcgcatattctagcgaaaagcaaactgtaaggagcgcaaat |
Promo 19 | pCAR (eGFP) | 745bp | 2900 | gtcccataccagtacaatgcaatactataaaaggacacccttagataatgtacagatgtcaccttggatgctcgtatagtgttgcactgtgcccagattaacacaattacccacacacatcctgggagaagggggaattctgcacattggctgtttcccaacagagtgcaagctctgagagcaaatcttgcccgtcattactgtttgatataaggtattcccatgcctgacgcacataatatttttgttacattcgtgtttacagcactgaaggcaggcagctatttgtgcagtcagtactttatgtatttctaaacccagtgctggagaggccaataggcaggatgccccccccgcgttagtccccctgcacaattgtgctgcacctgtctactccatttgcagacccctgtgtctgtgcaaactatttctttcattgtgctgttttttttgtcacccagcattacagacatgcttttttgggaatccctatttggccatccctagtagtgctccccccacctccatactttccatacatgggctaaggggtccaaagaccctgccctcccccctcacctactccattaatggcttctttgcttttcaatggcccagaagctaccaaataagggcaggctgcctgcctttccctgcggagctcccactgactcctcaactccaggcagcgtataaattgacagctcagtccccaggcagcacccagccaattcaatcacagcac | ||||
Promo 20 | p1 elastase GFP | Sequence |
June/July Box
Code | Name | Concentration (ng/ul) | Manipulator | Comments | Date |
J1 | Aux | RF | 14th june | ||
J2 | RFP | RF | 14th june | ||
J3 | Aux | 319,7 | RF | Miniprep | 19th june |
J4 | RFP | 101 | RF | 19th june | |
J5 | pCS2+ | TSO | DNA | 7th june | |
J6 | pCS2+ | 327,7 | TSO | 7th june | |
J7 | GFP | 209,1 | TSO | 28th june | |
J8 | YFP | 196 | TSO | 28th june | |
J9 | CFP | 240 | TSO | 28th june | |
J14 | Hsp70 | WR | PCR BB | 24th july | |
J15 | GFP AID | WR | PCR BB | 21th july | |
J16 | Tir1 | WR | Gel extract/PCR Fw | 27th july | |
J17 | Tir1 | WR | PCR RV, SV40 | 27th july | |
J18 | pCS2+ | WR | BB | 27th july | |
J19 | pNHK60 | 19th july | |||
J20 | IAA | WR | 27th july | ||
J21 | pCS2+ | ? |
August box
Code | Name | Concentration (ng/ul) | Manipulator | Comments | Date |
A1 | pCS2+ | TC | PCR purif | 3rd august | |
A2 | pCS2+ | TC | After PCR, Pst1/EcoRI digest + purification | 3rd august | |
A3 | pCS2+ mcherry | 20 ng total | NP | ||
A4 | pCS2+ dendra2 | 8 ng total | NP from Aurore | coilin | |
A5 | pCS2+ mcherry | 262 ng/ul | Jo | n°1 | 6th august |
A6 | pCS2+ mcherry | 264 ng/ul | Jo | n°2 | 6th august |
A7 | pCS2+ dendra2 | 193 ng/ul | Jo | n°1 | 6th august |
A8 | pCS2+ dendra2 | 340 ng/ul | Jo | n°2 | 6th august |
A9 | Nothing | ||||
A10 | pSB1C3 (IaaM&IaaH) | 207,1 ng/ul | KC | Midiprep elution 1ml | 9th august |
A11 | pNHK60 (Tir1-GFP-AID) | 2211,9 ng/ul | KC | gDNA extraction phenol/chloro | 10th august |
A12 | pNHK60 (Tir1-GFP-AID) | 50 ng/ul | KC | dilution gDNA extraction phenol/chloro | 13th august |
A13 | pNHK60 (Tir1-GFP-AID) | 25 ng/ul | KC | dilution gDNA extraction phenol/chloro | 13th august |
A14 | pCS2+ RFP | 706 ng/ul | KC | midiprep elution 400ul | 29th august |
Glycerol Stocks
Number | Strain | Plasmid | Description | Date |
P1 | E.Coli DH5 aplpha | pCS2+ | RFP ( BBA_J23100 ) | 13th july |
P2 | E.Coli DH5 aplpha | pCS2+ | Auxine () | 13th july |
P3 | E.Coli DH5 aplpha | pCS2+ | Tir1 GFP-Aid () | 19th july |
P4 | E.Coli DH5 aplpha | pCS2+ | Tir1 GFP-Aid () | 19th july |
P5 | E.Coli TOP 10 | pCS2+ | mcherry | 6th August-from Aurore |
P6 | E.Coli TOP 10 | pCS2+ | dendra2 | 6th August-from Aurore |
P7 | E.Coli TOP 10 | pSB1C3 | IaaM-IaaH (K515100) | 8th August |
P8 | E.Coli TOP 10 | p1 elastase | promoter elastase | 9th August |