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	<h2>Monday 3rd</h2>		<h2>Monday 3rd</h2>
-	'''[[	+	<u><h5>Construction about a new pCS2+ with RFP insert</u></h5>
-	Construction about a new pCS2+ with RFP insert :]]'''	+
-	-Digestion of the miniprep about pCSB1C3 with RFP insert  by EcoRI and PstI
-	-Purification gel to get only RFP insert ( gel image )	+	-Digestion of the miniprep about pCSB1C3 with RFP insert  by EcoRI and PstI <br>
		+
		+
		+	-Purification gel to get only RFP insert ( gel image )<br>
		+
		+
		+	-Extraction of the RFP insert <br>
-	-Extraction of the RFP insert
	-Ligation with pCS2+		-Ligation with pCS2+
		+
		+	<h5>Injection into tadpoles</h5>
		+
		+	Injection of plasmid pCS2+ with the CMV promoter and GFP-aid.
		+	This experiment was throughout the week. Taking pictures and take care of tadpole is a full-time work.
	<h2>Tuesday 3rd</h2>		<h2>Tuesday 3rd</h2>
-	'''[[
-	'''[[Preparation of a new stock of electrocompetent cells T10]]'''	+
		+	<u><h5>Preparation of a new stock of electrocompetent cells T10</u></h5>
		+
		+	<br>
		+	<br>
		+
		+	<u><h5>Construction about a new pCS2+ with RFP insert :</u></h5>
		+	<i>(Following)</i><br>
-	Construction about a new pCS2+ with RFP insert :]]'''	+	-Transformation by electroporation with T10 cells<br>
-	''Following''	+
-	-Transformation by electroporation with T10 cells
	-Plates prepared		-Plates prepared

---

Latest revision as of 03:06, 27 September 2012

Weeks:

June			July				August				September				October				November

Week 13: 3rd September - 9th September

Monday 3rd

Construction about a new pCS2+ with RFP insert

-Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI
-Purification gel to get only RFP insert ( gel image )
-Extraction of the RFP insert
-Ligation with pCS2+

Injection into tadpoles

Injection of plasmid pCS2+ with the CMV promoter and GFP-aid. This experiment was throughout the week. Taking pictures and take care of tadpole is a full-time work.

Tuesday 3rd

Preparation of a new stock of electrocompetent cells T10

Construction about a new pCS2+ with RFP insert :

(Following)
-Transformation by electroporation with T10 cells
-Plates prepared