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Team:Evry/Notebook/w14
From 2012.igem.org
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<li> FLk1</li> | <li> FLk1</li> | ||
<li>P CMV</li></ul> | <li>P CMV</li></ul> | ||
| - | this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing. | + | this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing. |
<li>Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+: | <li>Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+: | ||
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| - | + | ||
| - | <li>Preparation of chemical Competent Cells</li> | + | |
| + | <li>Preparation of chemical Competent Cells</li></ul> | ||
</ul> | </ul> | ||
Latest revision as of 00:20, 27 September 2012
Weeks:
| June | July | August | September | October | November | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Week 14: 10th September - 16th September
this week we have worked extensively on a batch of different manipulations including:
- biobricking promotors in Psb1c3 which are:
- P Elastase
- Hsp70
- P Car A
- FLk1
- P CMV
- Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+: this included PCR amplification of seperate genes GFP-AID and Tir1 using then apllying the golden gate protocol over night, followed by trsnformtion, sequencing and characterization of those of PCS2+ in tadpoles
- Preparation of chemical Competent Cells
