Team:Fatih-Medical/Timeline new

From 2012.igem.org

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<li id="week1" class="selected">
<li id="week1" class="selected">
<h1>Week 1</h1>
<h1>Week 1</h1>
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'''Friday'''
'''Friday'''
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* The results of P2L1 and P2L3 might be true , we are not sure about this so we need some expert assistance and we need to sequence these part for being sure. Unfortunately the result of L2 was wrong again.
* The results of P2L1 and P2L3 might be true , we are not sure about this so we need some expert assistance and we need to sequence these part for being sure. Unfortunately the result of L2 was wrong again.
* We prepared a new liquid culture for L2.
* We prepared a new liquid culture for L2.
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                                </p>
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<li id="week2">
<li id="week2">
<h1>Week 2</h1>
<h1>Week 2</h1>
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<p>Donec semper quam scelerisque tortor dictum gravida. In hac habitasse platea dictumst. Nam pulvinar, odio sed rhoncus suscipit, sem diam ultrices mauris, eu consequat purus metus eu velit. Proin metus odio, aliquam eget molestie nec, gravida ut sapien. Phasellus quis est sed turpis sollicitudin venenatis sed eu odio. Praesent eget neque eu eros interdum malesuada non vel leo. Sed fringilla porta ligula.</p>
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<img src="#" width=1 height=1>
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<p>'''Thursday'''
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* We made isolation for P2L1,P2L3,H1,C2,L2 and then digestion has been made with EcoR1 and Pst1 for all of these parts.After that electrophoresis was performed for these parts.
 +
* The results of P2L1 and P2L3 might be true , we are not sure about this so we need some expert assistance and we need to sequence these part for being sure. Unfortunately the result of L2 was wrong again.
 +
* We prepared a new liquid culture for L2.</p>
</li>
</li>
<li id="week3">
<li id="week3">
<h1>Week 3</h1>
<h1>Week 3</h1>
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<p>Donec semper quam scelerisque tortor dictum gravida. In hac habitasse platea dictumst. Nam pulvinar, odio sed rhoncus suscipit, sem diam ultrices mauris, eu consequat purus metus eu velit. Proin metus odio, aliquam eget molestie nec, gravida ut sapien. Phasellus quis est sed turpis sollicitudin venenatis sed eu odio. Praesent eget neque eu eros interdum malesuada non vel leo. Sed fringilla porta ligula.</p>
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</li>
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<p>'''Thursday'''
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<li id="1950">
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<img src="images/4.png" width="256" height="256" />
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* We made isolation for P2L1,P2L3,H1,C2,L2 and then digestion has been made with EcoR1 and Pst1 for all of these parts.After that electrophoresis was performed for these parts.
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<h1>1950</h1>
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* The results of P2L1 and P2L3 might be true , we are not sure about this so we need some expert assistance and we need to sequence these part for being sure. Unfortunately the result of L2 was wrong again.
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<p>Donec semper quam scelerisque tortor dictum gravida. In hac habitasse platea dictumst. Nam pulvinar, odio sed rhoncus suscipit, sem diam ultrices mauris, eu consequat purus metus eu velit. Proin metus odio, aliquam eget molestie nec, gravida ut sapien. Phasellus quis est sed turpis sollicitudin venenatis sed eu odio. Praesent eget neque eu eros interdum malesuada non vel leo. Sed fringilla porta ligula.</p>
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* We prepared a new liquid culture for L2.</p>
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<li id="1971">
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<img src="images/5.png" width="256" height="256" />
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<h1>1971</h1>
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<p>Donec semper quam scelerisque tortor dictum gravida. In hac habitasse platea dictumst. Nam pulvinar, odio sed rhoncus suscipit, sem diam ultrices mauris, eu consequat purus metus eu velit. Proin metus odio, aliquam eget molestie nec, gravida ut sapien. Phasellus quis est sed turpis sollicitudin venenatis sed eu odio. Praesent eget neque eu eros interdum malesuada non vel leo. Sed fringilla porta ligula.</p>
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</li>
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<li id="1977">
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<img src="images/6.png" width="256" height="256" />
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<h1>1977</h1>
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<p>Donec semper quam scelerisque tortor dictum gravida. In hac habitasse platea dictumst. Nam pulvinar, odio sed rhoncus suscipit, sem diam ultrices mauris, eu consequat purus metus eu velit. Proin metus odio, aliquam eget molestie nec, gravida ut sapien. Phasellus quis est sed turpis sollicitudin venenatis sed eu odio. Praesent eget neque eu eros interdum malesuada non vel leo. Sed fringilla porta ligula.</p>
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</li>
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<li id="1989">
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<img src="images/7.png" width="256" height="256" />
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<h1>1989</h1>
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<p>Donec semper quam scelerisque tortor dictum gravida. In hac habitasse platea dictumst. Nam pulvinar, odio sed rhoncus suscipit, sem diam ultrices mauris, eu consequat purus metus eu velit. Proin metus odio, aliquam eget molestie nec, gravida ut sapien. Phasellus quis est sed turpis sollicitudin venenatis sed eu odio. Praesent eget neque eu eros interdum malesuada non vel leo. Sed fringilla porta ligula.</p>
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</li>
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<li id="1999">
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<img src="images/8.png" width="256" height="256" />
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<h1>1999</h1>
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<p>Donec semper quam scelerisque tortor dictum gravida. In hac habitasse platea dictumst. Nam pulvinar, odio sed rhoncus suscipit, sem diam ultrices mauris, eu consequat purus metus eu velit. Proin metus odio, aliquam eget molestie nec, gravida ut sapien. Phasellus quis est sed turpis sollicitudin venenatis sed eu odio. Praesent eget neque eu eros interdum malesuada non vel leo. Sed fringilla porta ligula.</p>
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</li>
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<li id="2001">
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<img src="images/9.png" width="256" height="256" />
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<h1>2001</h1>
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<p>Donec semper quam scelerisque tortor dictum gravida. In hac habitasse platea dictumst. Nam pulvinar, odio sed rhoncus suscipit, sem diam ultrices mauris, eu consequat purus metus eu velit. Proin metus odio, aliquam eget molestie nec, gravida ut sapien. Phasellus quis est sed turpis sollicitudin venenatis sed eu odio. Praesent eget neque eu eros interdum malesuada non vel leo. Sed fringilla porta ligula.</p>
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</li>
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<li id="2011">
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<img src="images/10.png" width="256" height="256" />
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<h1>2011</h1>
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<p>Donec semper quam scelerisque tortor dictum gravida. In hac habitasse platea dictumst. Nam pulvinar, odio sed rhoncus suscipit, sem diam ultrices mauris, eu consequat purus metus eu velit. Proin metus odio, aliquam eget molestie nec, gravida ut sapien. Phasellus quis est sed turpis sollicitudin venenatis sed eu odio. Praesent eget neque eu eros interdum malesuada non vel leo. Sed fringilla porta ligula.</p>
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</li>
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</ul>
</ul>

Latest revision as of 22:41, 26 September 2012

  • Week 1

    Friday

    Today we started with a briefing from Mustafa where the main action points of the day were discussed. We had been planning the project of this year.We determined our parts that will be necessary:

    • K124014 holin
    • K559010 halorhodopsin+terminator(X2)
    • K112806 endolysine/T4
    • K112808 endolysine/holin/CMV/antiholin

    These 4 parts was diluted and tagged. Then we coded these parts:

    • C1:J23100 cons-promoter
    • C2:CMV promoter
    • P1:CL promoter
    • H1:Holorodopsin
    • P2:IPTG
    • L1:endolsin
    • L2:holin
    • l3:eth+antiholin


    We prepared 35 plates and 200 ml LB broth for next experiments.

    • Transformation (L1,L2,L3) 

    • C1,C2 streak
    • Coloniese are incubated in liquid culture.(C1)

    Saturday

    • Coloniese are incubated liquid culture.( 1,5 ml AMP ) for :

    C1(x2),C2,N1,L2,L3.

    • We made this process 2 times because of high mistake risk for first times

    Sunday

    • Single colony isolation has made for C1-x,C1-y,C2,L1,L2 and L3.
    • C1-x and C1-y are digested with EcoR1 and Pst1.
    • We prepared liquid culture for C2,L1,L3 again.Because there was not enough DNA for digestion.

    Monday

    • Single colony isolation has been made for L1,L2 and L3.
    • Digestion has been made with EcoR1 and Pst1 for L1,L3 and C2.
    • electrophoresis was performed for C1-x,C2,L1,L2 and L3.
    • After electrophoresis,results of L1,L3 was true but the others was wrong.

    Tuesday

    • Digestion has been made with Xba1 and Pst1 for L1 and L3.
    • 34 mg/ml chloramphenicol included 49 tubes was prepared.
    • Chloramphenicol included 17 plates was prepared.
    • IPTG induceble promoter was coded as P2.
    • Transformation has been made for H1,L1P2 and L3P2
    • We prepared liquid culture for C1,C2,L2

    Wednesday

    • Single colony isolation has been made for C1,C2,L2
    • We prepared liquid culture for C2 again.Because there was not enough DNA for digestion.
    • C1 and L2 are digested.
    • electrophoresis was performed for C1 and L2.
    • The result of C1 was true but the result of L2 was wrong so a new digestion will been performed and after that electrophoresis will been performed for new L2 and old L2.
    • We prepared liquid culture for P2L1,P2L3,C2,H1 and L2.

    Thursday

    • We made isolation for P2L1,P2L3,H1,C2,L2 and then digestion has been made with EcoR1 and Pst1 for all of these parts.After that electrophoresis was performed for these parts.
    • The results of P2L1 and P2L3 might be true , we are not sure about this so we need some expert assistance and we need to sequence these part for being sure. Unfortunately the result of L2 was wrong again.
    • We prepared a new liquid culture for L2.

  • Week 2

    '''Thursday''' * We made isolation for P2L1,P2L3,H1,C2,L2 and then digestion has been made with EcoR1 and Pst1 for all of these parts.After that electrophoresis was performed for these parts. * The results of P2L1 and P2L3 might be true , we are not sure about this so we need some expert assistance and we need to sequence these part for being sure. Unfortunately the result of L2 was wrong again. * We prepared a new liquid culture for L2.

  • Week 3

    '''Thursday''' * We made isolation for P2L1,P2L3,H1,C2,L2 and then digestion has been made with EcoR1 and Pst1 for all of these parts.After that electrophoresis was performed for these parts. * The results of P2L1 and P2L3 might be true , we are not sure about this so we need some expert assistance and we need to sequence these part for being sure. Unfortunately the result of L2 was wrong again. * We prepared a new liquid culture for L2.

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