Team:TU Darmstadt/Protocols/Glycerine stock

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In order to have a permanent culture of cells glycerine stocks can be made.
In order to have a permanent culture of cells glycerine stocks can be made.
=== Protocol ===
=== Protocol ===
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* Add 200 µl of sterilized glycerine to 800 µl cell culture and mix well
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* Add 200 µl of sterilized glycerine or DMSO to 800 µl cell culture and mix well
* Freeze the stock at -20 °C
* Freeze the stock at -20 °C

Latest revision as of 21:16, 26 September 2012

Glycerine stock

In order to have a permanent culture of cells glycerine stocks can be made.

Protocol

  • Add 200 µl of sterilized glycerine or DMSO to 800 µl cell culture and mix well
  • Freeze the stock at -20 °C