Team:TU Darmstadt/Materials/tctA503

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  rev: gtttcttcgaattcgcggccgcttctagatggatctgattcagaacttgtcgacg
  rev: gtttcttcgaattcgcggccgcttctagatggatctgattcagaacttgtcgacg
=== tctA503 Pst1 ===
=== tctA503 Pst1 ===
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[https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for removing a Pst1 site.
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[https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for removing a [https://2012.igem.org/Team:TU_Darmstadt/Materials/PstI Pst1] site.
       5'                            3'
       5'                            3'
  fwd: TGGTGCTGATGCCGGCAGTGAAGAACAAGCG
  fwd: TGGTGCTGATGCCGGCAGTGAAGAACAAGCG
  rev: CGCTTGTTCTTCACTGCCGGCATCAGCACCA
  rev: CGCTTGTTCTTCACTGCCGGCATCAGCACCA

Latest revision as of 20:51, 26 September 2012

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808002 tctA503]

Primers for the construction of a BioBrick containing the large subunit A1 of the tripartite tricarboxylate transporter family.

tctA503 BioBrick

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatggatctgattcagaacttgtcgacg
rev: gtttcttcgaattcgcggccgcttctagatggatctgattcagaacttgtcgacg

tctA503 Pst1

Primers for removing a Pst1 site.

     5'                             3'
fwd: TGGTGCTGATGCCGGCAGTGAAGAACAAGCG
rev: CGCTTGTTCTTCACTGCCGGCATCAGCACCA