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Team:TU Darmstadt/Materials/Ampicillin
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<li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | ||
<li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | ||
| - | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a | + | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li> |
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<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | ||
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=== About === | === About === | ||
Ampcillin is a beta-lactam [https://2012.igem.org/Team:TU_Darmstadt/Materials#Antibiotics antibiotic] that acts as a competitve inhibitor of transpeptidase an enzyme required for cell wall synthesis. Ampicillin works on most gram-positive and some gram-negative bacteria. | Ampcillin is a beta-lactam [https://2012.igem.org/Team:TU_Darmstadt/Materials#Antibiotics antibiotic] that acts as a competitve inhibitor of transpeptidase an enzyme required for cell wall synthesis. Ampicillin works on most gram-positive and some gram-negative bacteria. | ||
| + | === Stock === | ||
| + | # Mix: | ||
| + | #* 4 g ampicillin (100 mg/mL) | ||
| + | #* ad. 40 mL dist. water | ||
| + | # sterile filtration | ||
| + | # aliquot in 1 mL stocks and store at -20°C | ||
=== Stability === | === Stability === | ||
| - | * Ampicillin is not autoclavable, it has to be added before pouring plates (keep in mind that the agar is not too hot when adding it) | + | * Ampicillin is not autoclavable, it has to be added before [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Pouring_LB_plates pouring plates] (keep in mind that the agar is not too hot when adding it) |
* Culture plates can be stored for about 2 month at 4°C (as a batch in a bag or individually sealed with [http://en.wikipedia.org/wiki/Parafilm Parafilm]) | * Culture plates can be stored for about 2 month at 4°C (as a batch in a bag or individually sealed with [http://en.wikipedia.org/wiki/Parafilm Parafilm]) | ||
=== References === | === References === | ||
* http://en.wikipedia.org/wiki/Ampicillin | * http://en.wikipedia.org/wiki/Ampicillin | ||
Latest revision as of 20:42, 26 September 2012
Contents |
Ampicillin
About
Ampcillin is a beta-lactam antibiotic that acts as a competitve inhibitor of transpeptidase an enzyme required for cell wall synthesis. Ampicillin works on most gram-positive and some gram-negative bacteria.
Stock
- Mix:
- 4 g ampicillin (100 mg/mL)
- ad. 40 mL dist. water
- sterile filtration
- aliquot in 1 mL stocks and store at -20°C
Stability
- Ampicillin is not autoclavable, it has to be added before pouring plates (keep in mind that the agar is not too hot when adding it)
- Culture plates can be stored for about 2 month at 4°C (as a batch in a bag or individually sealed with [http://en.wikipedia.org/wiki/Parafilm Parafilm])
References
- http://en.wikipedia.org/wiki/Ampicillin
