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Team:TU Darmstadt/Materials/EcoRI
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5' GAATTC --> 5'---G AATTC---3' | 5' GAATTC --> 5'---G AATTC---3' | ||
3' CTTAAG --> 3'---CTTAA G---5' | 3' CTTAAG --> 3'---CTTAA G---5' | ||
| + | '''Buffer:''' [https://2012.igem.org/wiki/index.php?title=Team:TU_Darmstadt/Materials/NEB_buffers&action=submit#NEBuffer_EcoRI NEBuffer EcoRI] | ||
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EcoRI can exhibit star activity depending on the conditions present in the reaction and needs at least 1bp overhang on its cutting site.<sup>[2]</sup> | EcoRI can exhibit star activity depending on the conditions present in the reaction and needs at least 1bp overhang on its cutting site.<sup>[2]</sup> | ||
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=== Supplier === | === Supplier === | ||
* NEB http://www.neb.com/nebecomm/products/productR3101.asp | * NEB http://www.neb.com/nebecomm/products/productR3101.asp | ||
=== References === | === References === | ||
[1] http://en.wikipedia.org/wiki/EcoRI | [1] http://en.wikipedia.org/wiki/EcoRI | ||
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[2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp | [2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp | ||
Latest revision as of 20:37, 26 September 2012
Contents |
EcoRI
About
EcoRI is a endonucleasic restriction enzyme isolated from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli RY13]. It creates stick ends with 5' end overhang.
Working conditions
The recognition motive of EcoRI is:[1]
5' GAATTC --> 5'---G AATTC---3' 3' CTTAAG --> 3'---CTTAA G---5'
Buffer: NEBuffer EcoRI
EcoRI can exhibit star activity depending on the conditions present in the reaction and needs at least 1bp overhang on its cutting site.[2]
Supplier
- NEB http://www.neb.com/nebecomm/products/productR3101.asp
References
[1] http://en.wikipedia.org/wiki/EcoRI
[2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp
