Team:TU Darmstadt/Materials/Materials

From 2012.igem.org

(Difference between revisions)
(AppliChem GmbH)
(AppliChem GmbH)
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* yeast extract
* yeast extract
=== AppliChem GmbH  ===
=== AppliChem GmbH  ===
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* bromothymol blue)
+
* bromothymol blue
-
* tetramethylethylenediamine (TEMED
+
* tetramethylethylenediamine (TEMED)
=== Sigma-Aldrich Co. LLC. ===
=== Sigma-Aldrich Co. LLC. ===

Revision as of 20:28, 26 September 2012

Contents

Chemicals

CARL ROTH GMBH + CO. KG

  • acetic acid
  • acrylamide (rotiphorese)
  • agar-agar kobe I
  • ammonium persulfate (APS)
  • ammonium sulfate
  • ampicillin
  • ß-mercaptoethanol
  • chloramphenicol
  • coomassie brillant blue 250
  • D-(+)-glucose
  • ethanol
  • ethylenediaminetetraacetic acid (EDTA)
  • gloves
  • glycerol
  • HCl
  • imidazole
  • isopropyl alcohol
  • kanmycin
  • KCl
  • KH2PO4
  • L-(+)-arabinose
  • Na2HPO4
  • NaCl
  • NaOH
  • NiCl2
  • sodiumdodecylsulfate (SDS)
  • tributyrine
  • tricine
  • TRIS-HCl
  • tryptone
  • yeast extract

AppliChem GmbH

  • bromothymol blue
  • tetramethylethylenediamine (TEMED)

Sigma-Aldrich Co. LLC.

  • bromphenol blue
  • hydrocinnemonium chloride
  • para-nitrophenol
  • succinyl chloride
  • tween 20

New England Biolabs GmbH

Enzymes

  • EcoRI
  • EcoRI HF
  • NcoI HF
  • NdeI HF
  • NotI HF
  • Phusion
  • PstI HF
  • Q5 Polymerase Hotstart
  • Q5 Polymerase normal
  • T4 DNA Ligase
  • XbaI HF

Buffer

  • 2-log DNA marker
  • bovine serum albumin (BSA 100x)
  • Buffer 4
  • dimethylsulfoxide (DMSO)
  • DNA loading Dye 6x
  • GC Phusion 5x Buffer
  • GC Q5 Enhancer Buffer
  • MgCl2
  • Phusion HF Buffer 5x
  • Q5 reaction Buffer
  • T4 Ligase Buffer 10x

Promega GmbH

Enzymes

  • EcoRI
  • PstI
  • SpeI GQ
  • T4 Ligase
  • TEV Protease
  • XbaI GQ

Buffer

  • Buffer B
  • Buffer D
  • Buffer H
  • Go Tag Puffer 5x
  • Go Tag Puffer 5x prestained
  • Multi Core 10x Buffer
  • T4 Ligase Buffer 10x

Kits

  • Pure Yield Plasmid Miniprep System
  • Wizard SV Gel and PCR Clean Up System