Team:Nanjing-China/Attributions

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                 <a class="attr-link" href="https://2012.igem.org/Team:Nanjing-China/Attributions">Attributions</a>
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          <h2>Abstract</h2>
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            <h2><b><span lang="EN-US" style="font-size:12.0pt;font-family:仿宋">Attributions</span></b></h2>
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          <p>We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.</p>
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<h3><b><span lang="EN-US" style="font-size:12.0pt;font-family:仿宋">Attributions
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          <p>We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.</p>
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of undergraduate students</span></b></h3>
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          <p>In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.</p>
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<span lang="EN-US" style="font-size: 12.0pt; font-family: Century">Parts design
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and Molecular operation: Jia-Bao Chen &amp; Jia-Wei Gu</span></p>
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<p class="MsoNormal">
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<span lang="EN-US" style="font-size: 12.0pt; font-family: Century">Green plant
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transfection and culture: Lu-Xiao Chen, Yue-Chen Liu, Chu-Ze Shen, and Jian Gao</span></p>
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<p class="MsoNormal">
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<span lang="EN-US" style="font-size: 12.0pt; font-family: Century">Organization
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for human practice: Xin-Yue Luo, </span></p>
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<p class="MsoNormal">
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<span lang="EN-US" style="font-size: 12.0pt; font-family: Century">Measurement:
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Lu-Xiao Chen, Yue-Chen Liu, Chu-Ze Shen</span></p>
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<p class="MsoNormal">
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<span lang="EN-US" style="font-size: 12.0pt; font-family: Century">Wiki and  
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presentation design: Xin-Yue Luo, Jian Gao, Jia-Bao Chen</span></p>
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<p class="MsoNormal"><b>
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<span lang="EN-US" style="font-size:12.0pt;font-family:仿宋">&nbsp;</span></b></p>
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<h2><b><span lang="EN-US" style="font-size:12.0pt;font-family:仿宋">Works done by  
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others</span></b></h2>
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<p class="MsoNormal"><span style="font-size: 12.0pt; font-family: Century">
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Sequence of the original plant miRNA gene and cloning of this gene is provided
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by instructor Prof. Zhi Hong and graduate student Xiao Lin Sang.</span></p>
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<p class="MsoNormal"><span style="font-size: 12.0pt; font-family: Century">The
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guidance of plant transfection and tissue culture is also provided by our
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instructors. </span></p>
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<p class="MsoNormal"><span style="font-size: 12.0pt; font-family: Century">&nbsp;</span></p>
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<p class="MsoNormal"><span style="font-size: 12.0pt; font-family: Century">All
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the primers synthesis and gene sequencing were done by GenScript Company. </span>
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</p>
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<p class="MsoNormal"><span style="font-size: 12.0pt; font-family: Century">&nbsp;</span></p>
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<p class="MsoNormal"><span style="font-size: 12.0pt; font-family: Century">The
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pPZP 212 vector was purchased from BioVector Company.</span></p>
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Latest revision as of 20:16, 26 September 2012

Attributions

Attributions of undergraduate students

Parts design and Molecular operation: Jia-Bao Chen & Jia-Wei Gu

Green plant transfection and culture: Lu-Xiao Chen, Yue-Chen Liu, Chu-Ze Shen, and Jian Gao

Organization for human practice: Xin-Yue Luo,

Measurement: Lu-Xiao Chen, Yue-Chen Liu, Chu-Ze Shen

Wiki and presentation design: Xin-Yue Luo, Jian Gao, Jia-Bao Chen

 

Works done by others

Sequence of the original plant miRNA gene and cloning of this gene is provided by instructor Prof. Zhi Hong and graduate student Xiao Lin Sang.

The guidance of plant transfection and tissue culture is also provided by our instructors.

 

All the primers synthesis and gene sequencing were done by GenScript Company.

 

The pPZP 212 vector was purchased from BioVector Company.

Retrieved from "http://2012.igem.org/Team:Nanjing-China/Attributions"