Team:Shenzhen/Project/YAO.Suicider
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+ | <div class="context"> | ||
+ | <h5>Introduction</h5> | ||
+ | <ul><p>YAO is like a BOMBER and cell is like whoever plays with fire and get burst. Once the fuse is ignited, YAO sacrifices, and following cascade reaction will lead to the cell repression!</p></ul> | ||
+ | <ul><div class="figurep"> | ||
+ | [[File:add1.jpg]]</div></ul> | ||
+ | <ul><p>More than crippling of YAO by DNase I, we creatively couple it with apoptosis of yeast cell! | ||
+ | </p></ul></div> | ||
+ | <div class="context"> | ||
+ | |||
+ | <h5>Amazing Features:</h5> | ||
+ | <ul><li>1. Various Signaling?</li></ul> | ||
+ | <ul><p>This module ships signals from nucleus to mitochondria directly or reversely in four distinct ways, including T7 RNAP/T7 promoter coupling, peptide signaling, RTG pathway and cyt C-mediated apoptosis !</p></ul> | ||
+ | <ul><div class="figurep"> | ||
+ | [[File:add2.jpg]]</div> | ||
+ | </ul> | ||
+ | <ul><li>2. Biosafer?</li></ul> | ||
+ | <ul><p>To guarantee biosafety, the engineered biofactory will die with its carrier cell ! </p></ul> | ||
+ | <ul><div class="figurep"> | ||
+ | [[File:add3.jpg]]</div></ul> | ||
+ | <ul><li>3. Signal imitation? </li></ul> | ||
+ | <ul><p>T7 RNAP/T7 promoter is employed to imitate the signal given to YAO instead of biosensor.</p></ul> | ||
+ | <ul><div class="figurep"> | ||
+ | [[File:add4.jpg]]</div></ul> | ||
+ | <ul><li>4. Holin kill eukaryotic?</li></ul> | ||
+ | <ul><p>We try to illustrate that holin can punch not only the cytomembrane of bacteria but also the outer membrane of mitochondria-like YAO and ER to release cytC.</p></ul> | ||
+ | <ul><div class="figurep"> | ||
+ | [[File:add5.jpg]]</div></ul> | ||
+ | <ul><li>5. Special signaling from mitochondria to nucleus?</li></ul> | ||
+ | <ul><p>RTG pathway tell the cell that mt “Emergency~ Emergency~”, so cell supply it some replenishment.</p></ul> | ||
+ | <ul><div class="figurep"> | ||
+ | [[File:add6.jpg]]</div></ul> | ||
+ | </div> | ||
<div class="context"> | <div class="context"> | ||
<h5>Original Ideas</h5> | <h5>Original Ideas</h5> | ||
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<ul><div class="figurep"> | <ul><div class="figurep"> | ||
[[File:project_yao_suicider_p7.jpg]] | [[File:project_yao_suicider_p7.jpg]] | ||
- | <p>Figure 7. Verify that holing can make pores on the inner membrane of mitochondrion. This is the alterant of DNase causing mt dysfunction. And it can also produce a membrane without mtDNA.</p></div> | + | <p>Figure 7. Verify that holing can make pores on the inner membrane of mitochondrion. This is the alterant of DNase causing mt dysfunction. And it can also produce a membrane without mtDNA.</p></div></ul> |
<ul><li>For Host Cell Suicide Part</li></ul> | <ul><li>For Host Cell Suicide Part</li></ul> | ||
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</div> | </div> | ||
+ | <div class="context"> | ||
+ | <h5>References</h5> | ||
+ | <ul><p> | ||
+ | 1. Prindle, A. et al. A sensing array of radically coupled genetic ‘biopixels’. Nature. 2011;481:39-44.</p></ul> | ||
+ | <ul><p>2. Liu, Z. Butow, R. A. Mitochondrial retrograde signaling. Annu Rev Genet. 2006;40:159-85.</p></ul> | ||
+ | <ul><p>3. Shi, Y. Sun, J.Current advance in the topological structure and function of holin encoded by bacteriophage lambda. Wei Sheng Wu Xue Bao. 2012 ;52:141-5.</p></ul> | ||
+ | <ul><p>4. Agu, C. A et al. Bacteriophage-encoded toxins: the lambda-holin protein causes caspase-independent non-apoptotic cell death of eukaryotic cells. Cell Microbiol. 2007:1753. Lipton, S. A.</p></ul> | ||
+ | <ul><p>5. Bossy-Wetzel, E. Dueling activities of AIF in cell death versus survival: DNA binding and redox activity. Cell. 2002;111:147-50.</p></ul> | ||
+ | <ul><p>6. Buttner, S. Endonuclease G regulates budding yeast life and death. Mol Cell. 2007 ;25:233-46.</p></ul> | ||
+ | <ul><p>7. Spencer, S. L. Sorger, P. K. Measuring and modeling apoptosis in single cells. Cell. 2011;144:926-39.</p></ul> | ||
+ | <ul><p>8. Li, P. Nijhawan, D. Wang, X. Mitochondrial activation of apoptosis. Cell. 2004;116:S57-9.</p></ul> | ||
+ | <ul><p>9. Pinkham, J. L. Dudley, A. M. Mason, T. L. T7 RNA polymerase-dependent expression of COXII in yeast mitochondria. Mol Cell Biol. 1994 ;14:4643-52.</p></ul> | ||
+ | </div> | ||
</div> | </div> | ||
{{:Team:Shenzhen/Temp/gallery.htm}} | {{:Team:Shenzhen/Temp/gallery.htm}} |
Latest revision as of 17:50, 26 September 2012