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Team:Wageningen UR/Protocol
From 2012.igem.org
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<li>[[Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]</li> | <li>[[Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/StartupPolero|Growing culture]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/DialysisPolero|Dialysis of the VLPs]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/RoundupPolero|Purifing the VLPs]]</li> | ||
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Revision as of 15:25, 26 September 2012
Contents |
Methods
The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together.
Protocols
Medium & Buffer recipes
- LB medium
- SOB medium
- SOC medium
- Disassembly buffer
- Dialysis buffer 10x
- Reassembly buffer
- Virus buffer
- Formation Buffer HepBcAg
- Washing Buffer HepBcAg
- Towbin's electrotransfer buffer 1x
- Towbin's electrotransfer buffer 10x
CCMV Coat Protein VLP formation
Hepatitis B Coat Protein VLP formation
Polerovirus Coat Protein VLP formation
General Protocol
- Preparation of electrocompetent cells of E. coli
- SDS-Polyacrylamide and native gel electrophoresis
- Dynamic Light Scattering user manual
- French press user manual
- FPLC user manual
