Team:Shenzhen/Notebook/YAO.Genome

From 2012.igem.org

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<h5>Context 1</h5>
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<h5>Journal</h5>
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<li>Apr 17th, 2012</li>
<li>Apr 17th, 2012</li>

Revision as of 11:56, 26 September 2012




Journal
  • Apr 17th, 2012

    • YAO. Genome Seminar: Papers from Yu CHEN should be read CAREFULLY which are mainly about plasmid introduction to mitochondrial.

    Problems to be solved:

    1. Experiment principle;

    2. Why necessary to each step;

    3. Bacterial strain to be used;

    4. Culture to be used.

    Core Papers:

    1. Fox et al. SCIENCE, VOL. 240. Mitochondrial Transformation in Yeast by Bombardment with Microprojectiles.

    2. STEPHEN A. JOHNSTON et al. Proc. Natl. Acad. Sci. USA 85 (1988). Plasmids can stably transform yeast mitochondria lacking endogenous mtDNA

    Participants:

    Junguang XU, Qiwu XU, Haojun JIANG, Jie WANG, Qiong WU, Senwei TANG, Qinsi LIANG, Min OU.

  • Apr 20th, 2012

    • YAO. Genome experiment design for first part:

    Task assignment:

    1. Usage of Gene gun and fluorescence microscope

    2. Research on mitochondria transformation methods

    3. Research on Saccharomyces cerevisiae genome, especially transcription system.

    4. Research on plasmid transformation directly or after isolated mitochondria.

    5. Research on detection methods about whether transformation is succeed.

    Core paper:

    Nathalie Bonnefoy et al. METHODS IN CELL BIOLOGY, VOL. 80. Genetic Transformation of Saccharomyces cerevisiae and Chlamydomonas reinhardtii Mitochondria.

    Participants:

    Junguang XU, Qiwu XU, Haojun JIANG, Jie WANG, Qiong WU, Senwei TANG, Qinsi LIANG, Min OU.

  • Apr 21st, 2012

    • YAO. Genome experiment design first draft came out.

    Participants: Qiwu XU

  • May 5th, 2012

    • Primers and vectors designation.

    Participants: Qiwu XU, Min YANG, Yun WANG

  • May 24th, 2012

    • Junguang XU shared a correlated paper:

    de Jong et al. BMC Systems Biology 2010, 4:55 Methodology article Experimental and computational validation of models of fluorescent and luminescent reporter genes in bacteria.

  • May 27th, 2012

    • Start to synthesize primers, vectors and other parts, such as promoters and terminators.

    Participants: Qiwu XU, Min YANG, Yun WANG

  • Jun 8th, 2012

    • Culture media compilation

    Preparations for pour plate method and vectors transformation.

    Participants: Qiwu XU, Senwei TANG

  • Jun 9th, 2012

    • Transformation experiment

    Participants: Qiwu XU, Senwei TANG

  • Jun 10th, 2012

    • PCR and electrophoresis

    Bacterial growth

    Participants: Qiwu XU, Senwei TANG

  • Jun 11th, 2012

    • Sequencing

    Participants: Qiwu XU, Senwei TANG

  • Jun 12th, 2012

    • Assignments for this weeks experiments

    Participants: Qiwu XU

  • Jun 13th, 2012

    • Culture media compilation

    Night 20:00 - 22:00

    Yeast growth

    Participants: Qiwu XU, Senwei TANG

  • Jun 14th, 2012 Night

    • Pour plate method processed.

    Participants: Qiwu XU, Senwei TANG

  • Jun 15th, 2012 Night

    • Reagents preparation

    Participants: Qiwu XU, Senwei TANG

  • Jun 16th - 17th, 2012

    • Preparations for latter experiments

    Participants: Qiwu XU, Senwei TANG

  • Jul 2rd - Sep 1st, 2012

    • YAO. Genome story, pictures preparation and presentation design.

    Participants:

    Junguang XU, Qiwu XU, Haojun JIANG, Jie WANG, Qiong WU, Senwei TANG, Qinsi LIANG, Junjie Zhang, Min OU.

  • Sep 6th, 2012

    • Scripts, BioBricks and pictures preparation for Wiki

    Participants:

    Qiwu XU, Min OU.