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Team:Exeter/lab book/gibs/wk6
From 2012.igem.org
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<p>Supernatant discarded</p> | <p>Supernatant discarded</p> | ||
<p><a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#57B947" target="_blank"><u>Fermentas protocol</u></a></p> | <p><a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#57B947" target="_blank"><u>Fermentas protocol</u></a></p> | ||
| - | <p><b><i>Step 10</i></b>45µl milli-Q water added in place of elution buffer, followed by another 5 µl after incubation and centrifugation</p> | + | <p><b><i>Step 10</i></b> 45µl milli-Q water added in place of elution buffer, followed by another 5 µl after incubation and centrifugation</p> |
<p>Nanodropped for following results: </p><p> | <p>Nanodropped for following results: </p><p> | ||
Revision as of 10:41, 26 September 2012
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Operon Construction: 13th - 17th August 2012 **Tuesday 14.8.12**Transformation of wcfA-term (BBa_K764010) **Wednesday 15.8.12** Transferred wcfA-term colonies to LB(tet) medium **Thursday 16.8.12** wfcA-term transformed cell broth tubes centrifuged 10 minutes, 3901 rcf, 4 °C Supernatant discarded Step 10 45µl milli-Q water added in place of elution buffer, followed by another 5 µl after incubation and centrifugation Nanodropped for following results: (A) 329.7 ng/µl (B) 50.0 ng/µl (C) 206.6 ng/µl Made streak plates from culture dregs |
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