"
Team:Exeter/lab book/gibs/wk6
From 2012.igem.org
(Difference between revisions)
m |
|||
| Line 104: | Line 104: | ||
<td valign="top" width="850"> | <td valign="top" width="850"> | ||
<div style="text-align:justify"> | <div style="text-align:justify"> | ||
| - | <font face=" | + | <font face="Verdana" color="#1d1d1b" size="2"> |
| - | <font face=" | + | <font face="Verdana" color="#57b947" size="4"> |
<p><b><u>Operon Construction: 13th - 17th August 2012</u></b></p> | <p><b><u>Operon Construction: 13th - 17th August 2012</u></b></p> | ||
</font> | </font> | ||
<!<p><b>**Tuesday 14.8.12**</b></p><br><p> | <!<p><b>**Tuesday 14.8.12**</b></p><br><p> | ||
| - | </p><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:# | + | </p><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#57b947"><u>Transformation</u></a> of <i>wcfA</i>-term (BBa_K764010)</p><br> |
<p><b>**Wednesday 15.8.12**</b></p><br> | <p><b>**Wednesday 15.8.12**</b></p><br> | ||
| - | <p><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:# | + | <p><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#57b947"><u>Transferred <i>wcfA</i>-term colonies to LB(tet) medium</u></a></p><br> |
Revision as of 10:36, 26 September 2012
Follow our Blog
Follow us on Twitter
|
|
|
Operon Construction: 13th - 17th August 2012 **Tuesday 14.8.12**Transformation of wcfA-term (BBa_K764010) **Wednesday 15.8.12** Transferred wcfA-term colonies to LB(tet) medium **Thursday 16.8.12** wfcA-term transformed cell broth tubes centrifuged 10 minutes, 3901 rcf, 4 °C Supernatant discarded Step 1045µl milli-Q water added in place of elution buffer, followed by another 5 µl after incubation and centrifugation Nanodropped for following results: (A) 329.7 ng/µl (B) 50.0 ng/µl (C) 206.6 ng/µl Made streak plates from culture dregs |
|
