Team:Tuebingen/Results

From 2012.igem.org

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(Results)
(Results)
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= Results =
= Results =
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== Parts Statuses ==
* '''Pfet3, Panb1, lacZ''': PCR was not successful for many tries. For Pfet3, new primers were designed but there was no time left to complete this part.
* '''Pfet3, Panb1, lacZ''': PCR was not successful for many tries. For Pfet3, new primers were designed but there was no time left to complete this part.
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* '''Padh1, Psuc2, rox1, mig1, luciferase, mPR ''Danio rerio'', mPR ''Xenopus laevis''''': These parts are all available and digested. The ligation in both pSB1C3 and our pRS shuttle plasmids was so far not successful.
* '''Padh1, Psuc2, rox1, mig1, luciferase, mPR ''Danio rerio'', mPR ''Xenopus laevis''''': These parts are all available and digested. The ligation in both pSB1C3 and our pRS shuttle plasmids was so far not successful.
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== Identified Problems ==
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== Progress Illustraion ==
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The following illustraion gives a quick overview of the final status of our lab work.
[[File:Tue-labmap-results.png|600px|thumb|center|final status of our parts]]
[[File:Tue-labmap-results.png|600px|thumb|center|final status of our parts]]

Revision as of 10:25, 26 September 2012



Results

Contents


Parts Statuses

  • Pfet3, Panb1, lacZ: PCR was not successful for many tries. For Pfet3, new primers were designed but there was no time left to complete this part.
  • Tadh1: Tadh1 has been successfully amplified and ligation in pGEM vectors is under way.
  • pRS313, pRS315, pRS316: The shuttle plasmids are available and digested.
  • Padh1, Psuc2, rox1, mig1, luciferase, mPR Danio rerio, mPR Xenopus laevis: These parts are all available and digested. The ligation in both pSB1C3 and our pRS shuttle plasmids was so far not successful.

Identified Problems

Progress Illustraion

The following illustraion gives a quick overview of the final status of our lab work.

final status of our parts