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| = Results = | | = Results = |
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- | == The Constructor ==
| + | An overview of the results can be found [[Wageningen_UR/FinalOverview|here]] |
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- | More efficient cloning, publication, importance of computational work in SynBio
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- | {| class="wikitable"
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- | ! iGEM 2011 team
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- | ! iGEM project title
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- | ! # of TU's in the designed circuit
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- | ! # of used Biobrick Parts By team
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- | ! Recommended by the Constructor
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- | ! Processing time (min)
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- | |-
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- | | KAIST-Korea<sup>1</sup> || E. casso ||style="text-align:center;"| 2 ||style="text-align:center;"| 11 ||style="text-align:center;"| 7 ||style="text-align:center;"| < 1 min
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- | | UANL_Mty-Mexico<sup>2</sup> || S.C.I.E.N.C.E. : Simple Code Interpretation Enabling Circuit in E. coli ||style="text-align:center;"| 7 ||style="text-align:center;"| 26 ||style="text-align:center;"| 25 ||style="text-align:center;"| 674 min
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- | |-
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- | | XMU-China<sup>3</sup> || i-ccdB: intelligent Control of Cell Density in Bacteria ||style="text-align:center;"| 3 ||style="text-align:center;"| 6 ||style="text-align:center;"| 6 ||style="text-align:center;"| < 1 min
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- | | NTNU-Trondheim<sup>4</sup> || Red Fluorescent Stress Sensor ||style="text-align:center;"| 2 ||style="text-align:center;"| 5 ||style="text-align:center;"| 4 ||style="text-align:center;"| < 1 min
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- | | Wageningen UR<sup>5</sup> || The Synchroscillator: a Synchronized Oscillatory System ||style="text-align:center;"| 4 ||style="text-align:center;"| 7 ||style="text-align:center;"| 6 ||style="text-align:center;"| < 2 min
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- | |}
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- | 1 https://2011.igem.org/Team:KAIST-Korea
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- | 2 https://2011.igem.org/Team:UANL_Mty-Mexico
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- | 3 https://2011.igem.org/Team:XMU-China
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- | 4 https://2011.igem.org/Team:NTNU_Trondheim
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- | 5 https://2011.igem.org/Team:Wageningen_UR
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- | <html>
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- | <p><a href="http://www.jbioleng.org/content/6/1/14/abstract" title="" class="bigbutton bPublication"><br>Our publication of The Constructor at the Journal of Biological Engineering <b>[Highly Accessed]</b>.</a></p>
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- | <p><a href="https://static.igem.org/mediawiki/2012/a/a7/Poster_Constructor_A4.jpg" title="" class="bigbutton bPoster"><br>The poster of The Constructor, presented at the German Synthetic Biology Conference in May 2012 in Munich.</a></p>
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- | </html>
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- | == Plug and Apply System ==
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- | GFP-coil, coils, the idea
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- | == Outside Modifications ==
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- | * Designed the primers for amplification of the needed [[Team:Wageningen_UR/Journal/week15|constructs]]
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- | * Delivered the proof of principle that these primers work [link to framshift, Hugo]
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- | * Provided the templates for modifications and placed them in the [[Team:Wageningen_UR/Parts|partsregistry]]
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- | * Isolated the [[Team:Wageningen_UR/ObtainingthePoleroVLP|PLRV]] from nature which, in theory, should be easy to modify on the outside
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- | == Inside Modifications ==
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- | Negative CCMV, Frameshift
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- | == Virus Like particles ==
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- | Detection
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- | Isolation from nature
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- | *[[RNA isolation]]
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- | *[[RT-PCR]]
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- | *[[Addition of prefix and suffix]]
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- | *[[Transformation of E.coli]]
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- | *[[Isolation of plasmids]]
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- | *[[Sequencing of PLRV CP's]]
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- | *[[Alignment experiments]]
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- | == General ==
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- | We organised a [[Team:Wageningen_UR/miniSymposium|mini Symposium]]for all dutch teams in the begining of the competition to create a national network and eventualy an[[Team:Wageningen_UR/DiscoveryFestival|national effort]]
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- | Human Practices
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- | We went to present our project and [[Team:Wageningen_UR/TheConstructor|The Constructor]] at the Centre of Advanced Studies Synthetic Biology conference in Munich, where we learned a lot and get familiar with the German iGEM teams
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