From 2012.igem.org

Revision as of 08:15, 26 September 2012

• Home
• Team
  • The Team
  • Team Registration
• Project
  • 1. Introduction
  • 2. PnA System
  • 3. Virus-Like Particles
    • CCMV
    • HepB
    • Polerovirus Natural BioBrick
  • 4. Outside Modification
  • 5. Inside Modification
  • 6. Detection of VLPs
  • 7. Applications
  • 8. The Constructor
  • 9. Final Overview
• Human Practices
  • Human Practices Home
  • 1. Mini Symposium
  • 2. Visiting Secondary Schools
  • 3. Discovery Festival
    • Communication Science
  • 4. Stakeholders
  • 5. Munich CAS Conference
• Safety
  • Introduction
  • 1. General safety
  • 2. Virus-related safety
  • 3. Regulations
  • 4. Safety suggestions
  • 5. Safety of applications
• Modeling
  • Human Body Model
  • Tertiary folding prediction
  • VLP assembly model
• Attributions
• Parts
• Notebook
  • Journal
  • Protocols

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Last year's project: Synchronized Oscillatory System

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Results

Outside Modifications

• Designed the primers for amplification of the needed constructs
• Delivered the proof of principle that these primers work [link to framshift, Hugo]
• Provided the templates for modifications and placed them in the partsregistry
• Isolated the PLRV from nature which, in theory, should be easy to modify on the outside

Virus Like particles

Detection Isolation from nature

• RNA isolation
• RT-PCR
• Addition of prefix and suffix

General

• We organised a mini Symposium for all dutch teams in the begining of the competition to create a national network and eventualy a national effort
• We visited secondary schools to teach them about synthetic biology and let the make leafelets about synthetic biology.
• We went to present our project and The Constructor at the Centre of Advanced Studies Synthetic Biology conference in Munich, where we learned a lot and get familiar with the German iGEM teams
• We communicated to a graphical designer to obtain an kinetic graphical movie of our VLP formation, that is included in our introduction page movie.
• We involved a communication study in our discovery festival human practises, so that we do not only inform the public, but also will have results on how scientist communicate with the public.
• We have taken a number of inverviews with stakeholders to have an opinion of expert on our project, regulations around our project and about the idea of an open source environment