Team:KIT-Kyoto/Notebook-week5

From 2012.igem.org

(Difference between revisions)
m
 
(17 intermediate revisions not shown)
Line 1: Line 1:
-
{{KIT-Kyoto}}
 
{{KIT-Kyoto.Header}}
{{KIT-Kyoto.Header}}
 +
{{KIT-Kyoto}}
<html>
<html>
<head>
<head>
Line 63: Line 63:
     <ul class="navi">
     <ul class="navi">
         <li>
         <li>
-
             <div class="category"><img src="https://static.igem.org/mediawiki/2012/1/1f/Side_labnotekit.jpg" width="150" height="30"></div>
+
             <div class="category"><img src="https://static.igem.org/mediawiki/2012/d/db/Side_partskit.jpg" width="150" height="30"></div>
 +
            <ul class="menu">
 +
                <li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-week1p"><img src="https://static.igem.org/mediawiki/2012/1/1b/Side_week1kit.jpg" width="150" height="30"></a></li>
 +
                <li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-week2p"><img src="https://static.igem.org/mediawiki/2012/9/94/Side_week2kit.jpg" width="150" height="30"></a></li>
 +
                <li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-week3p"><img src="https://static.igem.org/mediawiki/2012/e/e0/Side_week3kit.jpg" width="150" height="30"></a></li>
 +
                <li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-week4p"><img src="https://static.igem.org/mediawiki/2012/3/31/Side_week4kit.jpg" width="150" height="30"></a></li>
 +
                <li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-week5p"><img src="https://static.igem.org/mediawiki/2012/e/e7/Side_week5kit.jpg" width="150" height="30"></a></li>
 +
            </ul>
 +
        </li>
 +
        <li>
 +
        <li>
 +
            <div class="category"><img src="https://static.igem.org/mediawiki/2012/e/eb/Side_tnfaip3kit.jpg" width="150" height="30"></div>
             <ul class="menu">
             <ul class="menu">
                 <li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-week1"><img src="https://static.igem.org/mediawiki/2012/1/1b/Side_week1kit.jpg" width="150" height="30"></a></li>
                 <li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-week1"><img src="https://static.igem.org/mediawiki/2012/1/1b/Side_week1kit.jpg" width="150" height="30"></a></li>
Line 93: Line 104:
<br>
<br>
</td>
</td>
 +
<td width="800px" valign="top"><div id="MIGI">
<td width="800px" valign="top"><div id="MIGI">
Line 99: Line 111:
The female-virgin flies (yw) and male flies (yw) were collected for mating with the microinjected males and females.
The female-virgin flies (yw) and male flies (yw) were collected for mating with the microinjected males and females.
<br><br>
<br><br>
-
<Table Border Cellspacing="0">
+
 
-
<Tr><Td>9/3 GAL4</Td><Td>40uL</Td></Tr>
+
 
-
<tr><td>M buffer(TOYOBO)</td><td>5uL</td></tr>
+
 
-
<Tr><Td>XbaⅠ(TOYOBO)</Td><Td>0.5uL</Td></Tr>
+
-
<tr><td>SpeⅠ(TOYOBO)</td><td>0.5uL</td></tr><tr>
+
-
<td>dH<sub>2</sub>O</td><td>4uL</td></tr>
+
-
<td>Total<td>50uL</td></tr>
+
-
</Table>
+
-
<br><BR>
+
-
<Table Border Cellspacing="0">
+
-
<Tr><Td>each DNA</Td><Td>40uL</Td></Tr>
+
-
<tr><td>3 buffer(NEB)</td><td>5uL</td></tr>
+
-
<Tr><Td>BglⅡ(NEB)</Td><Td>0.5uL</Td></Tr>
+
-
<td>dH<sub>2</sub>O</td><td>4.5uL</td></tr>
+
-
<td>Total<td>50uL</td></tr>
+
-
</Table>
+
-
<br><BR>
+
-
<Table Border Cellspacing="0">
+
-
<Tr><Td>9/3 GAL4</Td><Td>23uL</Td></Tr>
+
-
<tr><td>M buffer(TOYOBO)</td><td>10uL</td></tr>
+
-
<Tr><Td>XbaⅠ(TOYOBO)</Td><Td>1uL</Td></Tr>
+
-
<Tr><Td>SpeⅠ(TOYOBO)</Td><Td>1uL</Td></Tr>
+
-
<Tr><Td>CIP</Td><Td>0.5uL</Td></Tr>
+
-
<td>dH<sub>2</sub>O</td><td>64.5uL</td></tr>
+
-
<td>Total<td>100uL</td></tr>
+
-
</Table>
+
-
<br><BR>
+
-
<img src="https://static.igem.org/mediawiki/2012/b/b0/0904kit.png" width="500" height="300">
+
-
<br><br>
+
<h2>September 5th</h2>
<h2>September 5th</h2>
<br>
<br>
The female-virgin flies (yw) and male flies (yw) were collected and separately kept at 25℃ for mating with the microinjected males and females.
The female-virgin flies (yw) and male flies (yw) were collected and separately kept at 25℃ for mating with the microinjected males and females.
<br><br>
<br><br>
-
<Table Border Cellspacing="0">
+
 
-
<Tr><Td></Td><Td>EGFP</Td><Td>pSB1C3</Td></Tr>
+
 
-
<tr><td>DNA template</td><td>40uL</td><Td>23uL</Td></tr>
+
 
-
<Tr><Td>3 buffer</Td><Td>5uL</Td><Td>5uL</Td></Tr>
+
-
<tr><td>BglⅡ</td><td>0.5uL</td><Td>0.5uL</Td></tr><tr>
+
-
<td>dH<sub>2</sub>O</td><td>4.5uL</td><Td>21.5uL</Td></tr>
+
-
<td>Total<td>50uL</td><Td>50uL</Td></tr>
+
-
</Table>
+
-
<br><BR>
+
-
<img src="https://static.igem.org/mediawiki/2012/3/3a/0905akit.png" width="500" height="300">
+
-
<br><br>
+
-
<img src="https://static.igem.org/mediawiki/2012/6/63/0905bkit.png" width="500" height="300">
+
-
<br><br>
+
<h2>September 6th</h2>
<h2>September 6th</h2>
<br>
<br>
The female-virgin flies (yw) and male flies (yw) were collected and separately kept at 25℃ for mating with the microinjected males and females.
The female-virgin flies (yw) and male flies (yw) were collected and separately kept at 25℃ for mating with the microinjected males and females.
<br><br>
<br><br>
-
<img src="https://static.igem.org/mediawiki/2012/5/53/0906kit.png" width="500" height="300">
+
 
-
<br><br>
+
 
 +
 
<h2>September 7th</h2>
<h2>September 7th</h2>
<br>
<br>
The eclosed male and virgin female flies from microinjected embryos were collected and kept at 25℃ for maturation.
The eclosed male and virgin female flies from microinjected embryos were collected and kept at 25℃ for maturation.
<br><br>
<br><br>
-
<img src="https://static.igem.org/mediawiki/2012/4/4c/0907akit.png" width="500" height="300">
+
 
-
<br><br>
+
 
-
<img src="https://static.igem.org/mediawiki/2012/6/68/0907bkit.png" width="500" height="300">
+
-
<br><br>
+
-
<img src="https://static.igem.org/mediawiki/2012/9/9e/0907ckit.png" width="500" height="300">
+
-
<br><br>
+
<h2>September 8th</h2>
<h2>September 8th</h2>
<br>
<br>
 +
The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. The eclosed male and virgin female flies from microinjected embryos were again collected and kept at 25℃ for maturation. The female-virgin flies (yw) and male flies (yw) were collected and separately kept at 25℃.
The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. The eclosed male and virgin female flies from microinjected embryos were again collected and kept at 25℃ for maturation. The female-virgin flies (yw) and male flies (yw) were collected and separately kept at 25℃.
<br><br>
<br><br>
 +
 +
 +
<h2>September 9th</h2>
<h2>September 9th</h2>
<BR>
<BR>
The eclosed male and virgin female flies from microinjected embryos were again collected and kept at 25℃ for maturation. In total 83 flies were eclosed from the microinjected embryos. The calculated viability for the microinjected flies was 12.0%. The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃.
The eclosed male and virgin female flies from microinjected embryos were again collected and kept at 25℃ for maturation. In total 83 flies were eclosed from the microinjected embryos. The calculated viability for the microinjected flies was 12.0%. The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃.
-
<br><br>
+
<br>
-
<img src="https://static.igem.org/mediawiki/2012/1/10/0909akit.png" width="500" height="300">
+
<br>
-
<br><br>
+
Drosophila S2 cells (2.5 X 10<sup>5</sup> cells per well) were plated on the 24 well plate and cultured in the Schneider’s Drosophila medium containing 10% fetal bovine serum at 25 ℃.
-
<img src="https://static.igem.org/mediawiki/2012/9/91/0909bkit.png" width="500" height="300">
+
-
<br><br>
+
-
<img src="https://static.igem.org/mediawiki/2012/b/bb/0909ckit.png" width="500" height="300">
+
<br><br>
<br><br>
 +
 +
<h2>September 10th</h2>
<h2>September 10th</h2>
<br>
<br>
The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible.
The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible.
-
<br><br>
+
<br>
-
<img src="https://static.igem.org/mediawiki/2012/2/27/0910akit.png" width="500" height="300">
+
<br>
-
<br><br>
+
 
-
<img src="https://static.igem.org/mediawiki/2012/e/eb/0910bkit.png" width="500" height="300">
+
 
-
<br><br>
+
<div align="right"><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-week6">>>>>>>>>>WEEK6</a></div>
-
<img src="https://static.igem.org/mediawiki/2012/8/86/0910ckit.png" width="500" height="300">
+
-
<br><br>
+
-
<img src="https://static.igem.org/mediawiki/2012/3/39/0910dkit.png" width="500" height="300">
+
-
<br><br>
+
-
<img src="https://static.igem.org/mediawiki/2012/6/6d/0910ekit.png" width="500" height="300">
+
-
<br><br>
+
</div>
</div>
</div>
</div>

Latest revision as of 04:14, 26 September 2012

September 4th


The female-virgin flies (yw) and male flies (yw) were collected for mating with the microinjected males and females.

September 5th


The female-virgin flies (yw) and male flies (yw) were collected and separately kept at 25℃ for mating with the microinjected males and females.

September 6th


The female-virgin flies (yw) and male flies (yw) were collected and separately kept at 25℃ for mating with the microinjected males and females.

September 7th


The eclosed male and virgin female flies from microinjected embryos were collected and kept at 25℃ for maturation.

September 8th


The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. The eclosed male and virgin female flies from microinjected embryos were again collected and kept at 25℃ for maturation. The female-virgin flies (yw) and male flies (yw) were collected and separately kept at 25℃.

September 9th


The eclosed male and virgin female flies from microinjected embryos were again collected and kept at 25℃ for maturation. In total 83 flies were eclosed from the microinjected embryos. The calculated viability for the microinjected flies was 12.0%. The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃.

Drosophila S2 cells (2.5 X 105 cells per well) were plated on the 24 well plate and cultured in the Schneider’s Drosophila medium containing 10% fetal bovine serum at 25 ℃.

September 10th


The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible.

Retrieved from "http://2012.igem.org/Team:KIT-Kyoto/Notebook-week5"