Team:UNITN-Trento/Parts

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<title>Our Parts</title>
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<li class="activeLink"><a href="https://2012.igem.org/Team:UNITN-Trento">Home</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento">Home</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/CrustAway">Crust Away</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/CrustAway">Crust Away</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/Terminators">Terminator 5</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/Terminators">Terminator 5</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Attributions">Attributions</a></li>
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<li class="folder"><a href="#">Parts</a>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/DataPage">Data Page</a></li>
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<li class="folder activeLink"><a href="javascript:void(0);">Parts</a>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Achievements">Achievements</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Achievements">Judging</a></li>
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<li class="folder"><a href="#">Team</a>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Team/Supervisors">Super- & Ad- visors</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Team/Supervisors">Super- & Ad- visors</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Friends">Our Friends</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Friends">Our Friends</a></li>
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<li><a href="#">Team Profile</a></li>
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<li><a href="https://igem.org/Team.cgi?id=731">Team Profile</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Trento">Where We Are</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Trento">Where We Are</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Gallery">Gallery</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Press">Press</a></li>
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<li class="folder"><a href="javascript:void(0);">Lab Notes</a>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Meetings">Meetings</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Meetings">Meetings</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">Protocols</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">Protocols</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">H<sub>2</sub>S</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Tools">Tools</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">Utilities</a></li>
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<li class="folder"><a href="javascript:void(0);">Safety</a>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Safety">Safety</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/H2S">H<sub>2</sub>S</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Safety">Safety</a></li>
 
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Outreach">Outreach</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Outreach">Outreach</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Art">Art&Science</a></li>
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<div id="logo">
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      <h1><a href="#" style="font-family: 'Bree Serif';">iGEM Trento 2012</a></h1>
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      <h1><a href="https://2012.igem.org/Team:UNITN-Trento" style="font-family: 'Bree Serif';"><span>i</span>GEM Trento 2012</a></h1>
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<div id="igem" style="position: absolute; right: 25px; top: 205px; width: 80px; z-index: 10;"><a href="https://igem.org/Main_Page"><img src="http://www.science.unitn.it/~igem/img/sponsor/iGEM.png" width="75px" alt="" /></a></div>
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<p>Here you can find all the Parts we built. Be sure to check out our <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=UNITN-Trento">Team Parts Page on the Registry website</a>!</p>
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<p>Here you can find our Best Parts and all the Parts we built. Make sure to check out our <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=UNITN-Trento">Team Parts Page on the Registry website</a>!</p>
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<table class="pgrouptable addborder" style="width: 925px;" cellpadding="0" cellspacing="0"><tbody><tr><th style="width: 7em;">Name</th><th colspan="">Type</th><th colspan="">Description</th><th colspan="">Designer</th><th class="blank_col">
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<h2 style="text-align: center;">BEST PARTS</h2>
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<div class="favPart" style="border-left: 7px solid #50aae4; padding-left: 10px; margin-bottom: 40px;">
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">K731030 - Inducible araC-pBAD promoter regulating M256I CysE</a></h2>
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<p>A serine acetyltransferase (CysE) controlled by an arabinose inducible system. <br/>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data">
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This is a key part in our black crust project: a device used to reduce sulfate and produce cysteine. </p>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25292"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></form></th></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731000">BBa_K731000</a></td><td>Coding</td><td>CysE (Serine Acetyltransferase)</td><td >Jason Fontana, Daniele Rossetto, Giacomo Giacomelli</td>
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<!--<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>-->
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</div>
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<div class="favPart" style="border-left: 7px solid #67f853; padding-left: 10px; margin-bottom: 40px;">
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">K731400 - IPTG inducible Cysteine desulfhydrase (CysDes)</a></h2>
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<p>Our favorite BioBrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key part for the Crust Away project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid.</p>
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<!--<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>-->
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</div>
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<div class="favPart" style="border-left: 7px solid #ffae00; padding-left: 10px; margin-bottom: 40px;">
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<div class="star"></div>
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<h2 class="orange"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">K731722 - rrnB T1 terminator</a></h2>
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<p>Our new entry in the terminator collection of the Registry: a functioning version of <a href="http://partsregistry.org/Part:BBa_B0010">BBa_B0010</a>! This terminator was characterized with both T7 and <i>E. coli</i> RNA polymerases. </p>
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<!--<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>-->
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</div>
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<hr>
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<h2 style="text-align: center;"> All Our Parts</h2>
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<p>Please note that many parts have been also subcloned into low copy vectors. Although these parts have note been submitted to the registry, they are available upon request. Contact us at igemtrento [at] gmail [dot] com</p>
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<h3>The Black Crust Enzymes</h3>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<div class="Part blue">
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25430"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731010">BBa_K731010</a></td><td>Coding</td><td>M256I CysE (Serine Acetyltransferase)</td><td >Jason Fontana, Daniele Rossetto, Giacomo Giacomelli</td>
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731000">K731000 CysE (Serine Acetyltransferase)</a></h2>
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<p>A Part encoding CysE, our key enzyme in the reduction of sulfate.</p>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25431"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731020">BBa_K731020</a></td><td>Composite</td><td>Inducible araC-pBAD promoter regulating WT CysE</td><td >Jason Fontana</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<div class="Part blue">
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25432"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">BBa_K731030</a></td><td>Composite</td><td>Inducible araC-pBAD promoter regulating M256I CysE</td><td >Jason Fontana, Daniele Rossetto</td>
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731010">K731010 M256I CysE (Serine Acetyltransferase)</a></h2>
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<p>This is a mutated CysE. Introduction of a Methionine at position 256, makes our key player even "stronger" and enhances sulfate uptake.
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25433"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731040">BBa_K731040</a></td><td>Composite</td><td>Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE</td><td >Jason Fontana, Daniele Rossetto</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<div class="Part blue">
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25355"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731201">BBa_K731201</a></td><td>Regulatory</td><td>Inducible araC-pBAD promoter</td><td >Daniele Rossetto</td>
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731020">K731020 Inducible araC-pBAD promoter regulating WT CysE</a></h2>
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<p>A device to produce CysE upon induction with arabinose. Also available in pSB3C5.
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25356"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731250">BBa_K731250</a></td><td>Composite</td><td>Inducible araC-pBAD promoter with GFPmut3b, strong RBS</td><td >Daniele Rossetto</td>
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<div class="Part blue" >
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25357"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731255">BBa_K731255</a></td><td>Composite</td><td>Inducible araC-pBAD promoter with GFPmut3b, weak RBS</td><td >Daniele Rossetto</td>
+
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">K731030 Inducible araC-pBAD promoter regulating M256I CysE</a></h2>
-
+
<p>A device for the production of M256I CysE controlled by an arabinose inducible cassette. This Part was used for the bioremoval of the black crust from different stones samples. Also available in pSB3C5.</p>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
</div>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25439"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731300">BBa_K731300</a></td><td>Regulatory</td><td>LacI + LacIq promoter (reverse)</td><td >Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli</td>
+
-
+
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<div class="Part blue">
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25440"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">BBa_K731400</a></td><td>Composite</td><td>IPTG inducible Cysteine desulfhydrase (CysDes)</td><td >Andrea Tassinari</td>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731040">K731040 Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE</a></h2>
-
+
<p>This is our favorite Part <a href="http://partsregistry.org/Part:BBa_K731030">BBa_K731030</a> followed by a sfGFP. Created by Gibson assembly.
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
</p>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25444"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731480">BBa_K731480</a></td><td>Composite</td><td>IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)</td><td >Andrea Tassinari, Francesco Guzzonato</td>
+
-
+
</div>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25446"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731500">BBa_K731500</a></td><td>Regulatory</td><td>Inducibile tac promoter and lac operon</td><td >Francesco Guzzonato</td>
+
-
+
<div class="Part green" >
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">K731400 IPTG inducible Cysteine desulfhydrase (CysDes)</a></h2>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25447"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731520">BBa_K731520</a></td><td>Composite</td><td>lactose-inducible sfGFP</td><td >Francesco Guzzonato</td>
+
<p>Our favorite Bi Bbrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key Part for the Crust Away project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid. Also available in pSB4K5.</p>
-
+
</div>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25452"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731600">BBa_K731600</a></td><td>Coding</td><td>Cysteine Desulfhydrase (CysDes)</td><td >Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli</td>
+
<div class="Part green">
-
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731480">K731480 IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)</a></h2>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<p>This is a sfGFP tagged version of our best BioBrick BBa_K731400. A superfluo device created by Gibson assembly.  Also available in pSB4K5.
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25409"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">BBa_K731700</a></td><td>Plasmid_Backbone</td><td>Low copy vector for terminators analysis with T7 RNApolymerase by fluorimetric assay</td><td >Giacomo Giacomelli, Anna Depetris</td>
+
</p>
-
+
</div>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25465"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731701">BBa_K731701</a></td><td>Measurement</td><td>BBa_K731700 with T7terminator (BBa_K731721)</td><td >Giacomo Giacomelli, Anna Depetris</td>
+
-
+
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<h3>Terminators</h3>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25466"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731702">BBa_K731702</a></td><td>Measurement</td><td>BBa_K731700 with BBa_K731722 terminator (BBa_B0010)</td><td >Giacomo Giacomelli, Anna Depetris</td>
+
-
+
<div class="Part orange">
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731721">K731721 T7 terminator</a></h2>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25411"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731710">BBa_K731710</a></td><td>Plasmid_Backbone</td><td>Low copy vector for terminators analysis with E. coli RNA polymerase by fluorimetric assay</td><td >Giacomo Giacomelli, Anna Depetris</td>
+
<p>Our new entry in the terminator collections of the Registry: a T7 terminator! This terminator was characterized in vivo and in vitro with both T7 and <i>E. coli</i> polymerases. </p>
-
+
</div>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25464"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731711">BBa_K731711</a></td><td>Measurement</td><td>BBa_K731710 with T7terminator (BBa_K731721)</td><td >Giacomo Giacomelli, Anna Depetris</td>
+
<div class="Part orange">
-
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">K731722 rrnB T1 terminator <i>E. coli</i> terminator</a></h2>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<p>A good working alternative for the E.coli terminator <a href="http://partsregistry.org/Part:BBa_B0010">BBa_B0010</a>. </p>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25462"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731712">BBa_K731712</a></td><td>Measurement</td><td>BBa_K731710 with BBa_K731722 terminator (BBa_B0010)</td><td >Giacomo Giacomelli, Anna Depetris</td>
+
</div>  
-
+
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<div class="Part orange">
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25413"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731721">BBa_K731721</a></td><td>Terminator</td><td>T7 terminator</td><td >Anna Depetris, Giacomo Giacomelli</td>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">K731700 Platform for terminators analysis under the control of T7 promoter</a></h2>
-
+
<p>Our measurement device for terminators characterization. A T7 promoter controlling the expression of A206K Venus and mCherry for the ratiometric analysis of terminator efficiency.</p>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
</div>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25455"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">BBa_K731722</a></td><td>Terminator</td><td>B0010 E. coli terminator</td><td >Anna Depetris, Giacomo Giacomelli</td>
+
-
+
<div class="Part orange">
-
</tr></tbody></table>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731710">K731710 Platform for terminators analysis under the control of TAC promoter</a></h2>
-
+
<p>Our great platform <a href="http://partsregistry.org/Part:BBa_K731722">BBa_K731722</a>, where T7 promoter was substituted with Ptac.</p>
-
+
</div>
 +
 +
<div class="Part orange">
 +
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731701">K731701 BBa_K731700 with T7terminator (BBa_K731721)</a></h2>
 +
<p>A measurement device used to characterize T7 terminator under the control of T7 promoter.</p>
 +
</div>
 +
 +
<div class="Part orange">
 +
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731702">K731702 BBa_K731700 with BBa_K731722 rrnB T1 terminator (BBa_B0010)</a></h2>
 +
<p>A measurement device used to characterize B0010 terminator under the control of T7 promoter.</p>
 +
</div>
 +
 +
<div class="Part orange">
 +
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731711">K731711 BBa_K731710 with T7 terminator (BBa_K731721)</a></h2>
 +
<p>A measurement device used to characterize the T7 terminator (<a href="http://partsregistry.org/Part:BBa_K731721">BBa_K731721</a>) under the control of tac promoter.</p>
 +
</div>
 +
 +
<div class="Part orange">
 +
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731712">K731712 BBa_K731710 with BBa_K731722 rrnB T1 terminator (<a href="http://partsregistry.org/Part:BBa_B0010">BBa_B0010</a>)</a></h2>
 +
<p>A measurement device used to characterize the E.coli terminator (<a href="http://partsregistry.org/Part:BBa_K731722">BBa_K731722</a>) under the control of tac promoter.</p>
 +
</div>
 +
 +
 +
<h3>Promoters</h3>
 +
 +
<div class="Part blue">
 +
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731201">K731201 Inducible araC-pBAD promoter</a></h2>
 +
<p>A new version of the araC-pBad promoter that was amplified from the E.coli genome. An excellent alternative to <a href="http://partsregistry.org/Part:BBa_I0050">BBa_I0050</a>.</p>
 +
</div>
 +
 +
<div class="Part green">
 +
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731300">K731300 LacI + LacIq promoter (reverse)</a></h2>
 +
<p>A ready to go IPTG inducible cassette that is composed of LacI controlled by the lacIq promoter, both in the reverse direction.</p>
 +
</div>
 +
 +
<div class="Part green">
 +
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731500">K731500 [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward</a></h2>
 +
<p>Our Part <a href="http://partsregistry.org/Part:BBa_K731300">BBa_K731300</a> followed by a tac promoter and a lac operator. The perfect BioBrick for those interested in expressing their gene under the control of IPTG. It will save you a lot of cloning steps! Also available in pSB4K5.
 +
</p>
 +
</div>
 +
 +
<div class="Part blue">
 +
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731250">K731250 Inducible araC-pBAD promoter with GFPmut3b, strong RBS</a></h2>
 +
<p>A device for the characterization of our new entry araC-pBad (<a href="http://partsregistry.org/Part:BBa_K731201">BBa_K731201</a>) followed by a strong RBS and a GFPmut3b. Also available in pSB3C5.</p>
 +
</div>
 +
 +
<div class="Part blue" >
 +
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731255">K731255 Inducible araC-pBAD promoter with GFPmut3b, weak RBS</a></h2>
 +
<p>A device for the characterization of our new entry araC-pBad (<a href="http://partsregistry.org/Part:BBa_K731201">BBa_K731201</a>) followed by a weak RBS and GFPmut3b.</p>
 +
</div>
 +
<div class="Part green">
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731520">K731520 IPTG inducible GFPmut3b</a></h2>
 +
<p>A device for the characterization of our new <a href="http://partsregistry.org/Part:BBa_K731500">BBa_K731500</a> followed by a strong RBS and GFPmut3b. Also available in pSB4K5.</p>
 +
</div>
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<li><a href="http://www.fondazionecassaruraleditrento.it"><img src="http://www.science.unitn.it/~igem/img/sponsor/CRT.jpg" alt="" /></a></li>
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<li><a href="http://www.unitn.it/en/cibio"><img src="http://www.science.unitn.it/~igem/img/sponsor/CIBIO.png" alt="" style="height:50px"/></a></li>
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<li><a href="https://2012.igem.org/Special:RecentChanges">Recent Changes</a></li>
<li><a href="https://2012.igem.org/Special:RecentChanges">Recent Changes</a></li>
<!--<li><a href="https://2012.igem.org/Special:WhatLinksHere/Team:UNITN-Trento/Parts">What links here</a></li>-->
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Latest revision as of 17:34, 25 September 2012

Our Parts

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Our Parts

Here you can find our Best Parts and all the Parts we built. Make sure to check out our Team Parts Page on the Registry website!

BEST PARTS

K731030 - Inducible araC-pBAD promoter regulating M256I CysE

A serine acetyltransferase (CysE) controlled by an arabinose inducible system.
This is a key part in our black crust project: a device used to reduce sulfate and produce cysteine.

K731400 - IPTG inducible Cysteine desulfhydrase (CysDes)

Our favorite BioBrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key part for the Crust Away project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid.

K731722 - rrnB T1 terminator

Our new entry in the terminator collection of the Registry: a functioning version of BBa_B0010! This terminator was characterized with both T7 and E. coli RNA polymerases.

All Our Parts

Please note that many parts have been also subcloned into low copy vectors. Although these parts have note been submitted to the registry, they are available upon request. Contact us at igemtrento [at] gmail [dot] com

The Black Crust Enzymes

K731000 CysE (Serine Acetyltransferase)

A Part encoding CysE, our key enzyme in the reduction of sulfate.

K731010 M256I CysE (Serine Acetyltransferase)

This is a mutated CysE. Introduction of a Methionine at position 256, makes our key player even "stronger" and enhances sulfate uptake.

K731020 Inducible araC-pBAD promoter regulating WT CysE

A device to produce CysE upon induction with arabinose. Also available in pSB3C5.

K731030 Inducible araC-pBAD promoter regulating M256I CysE

A device for the production of M256I CysE controlled by an arabinose inducible cassette. This Part was used for the bioremoval of the black crust from different stones samples. Also available in pSB3C5.

K731040 Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE

This is our favorite Part BBa_K731030 followed by a sfGFP. Created by Gibson assembly.

K731400 IPTG inducible Cysteine desulfhydrase (CysDes)

Our favorite Bi Bbrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key Part for the Crust Away project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid. Also available in pSB4K5.

K731480 IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)

This is a sfGFP tagged version of our best BioBrick BBa_K731400. A superfluo device created by Gibson assembly. Also available in pSB4K5.

Terminators

K731721 T7 terminator

Our new entry in the terminator collections of the Registry: a T7 terminator! This terminator was characterized in vivo and in vitro with both T7 and E. coli polymerases.

K731722 rrnB T1 terminator E. coli terminator

A good working alternative for the E.coli terminator BBa_B0010.

K731700 Platform for terminators analysis under the control of T7 promoter

Our measurement device for terminators characterization. A T7 promoter controlling the expression of A206K Venus and mCherry for the ratiometric analysis of terminator efficiency.

K731710 Platform for terminators analysis under the control of TAC promoter

Our great platform BBa_K731722, where T7 promoter was substituted with Ptac.

K731701 BBa_K731700 with T7terminator (BBa_K731721)

A measurement device used to characterize T7 terminator under the control of T7 promoter.

K731702 BBa_K731700 with BBa_K731722 rrnB T1 terminator (BBa_B0010)

A measurement device used to characterize B0010 terminator under the control of T7 promoter.

K731711 BBa_K731710 with T7 terminator (BBa_K731721)

A measurement device used to characterize the T7 terminator (BBa_K731721) under the control of tac promoter.

K731712 BBa_K731710 with BBa_K731722 rrnB T1 terminator (BBa_B0010)

A measurement device used to characterize the E.coli terminator (BBa_K731722) under the control of tac promoter.

Promoters

K731201 Inducible araC-pBAD promoter

A new version of the araC-pBad promoter that was amplified from the E.coli genome. An excellent alternative to BBa_I0050.

K731300 LacI + LacIq promoter (reverse)

A ready to go IPTG inducible cassette that is composed of LacI controlled by the lacIq promoter, both in the reverse direction.

K731500 [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward

Our Part BBa_K731300 followed by a tac promoter and a lac operator. The perfect BioBrick for those interested in expressing their gene under the control of IPTG. It will save you a lot of cloning steps! Also available in pSB4K5.

K731250 Inducible araC-pBAD promoter with GFPmut3b, strong RBS

A device for the characterization of our new entry araC-pBad (BBa_K731201) followed by a strong RBS and a GFPmut3b. Also available in pSB3C5.

K731255 Inducible araC-pBAD promoter with GFPmut3b, weak RBS

A device for the characterization of our new entry araC-pBad (BBa_K731201) followed by a weak RBS and GFPmut3b.

K731520 IPTG inducible GFPmut3b

A device for the characterization of our new BBa_K731500 followed by a strong RBS and GFPmut3b. Also available in pSB4K5.

Retrieved from "http://2012.igem.org/Team:UNITN-Trento/Parts"