Team:UNITN-Trento/Parts

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<title>Our Parts</title>
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<h2 style="font-family: 'Open Sans'; line-height: 40px; color:#ffae00; padding:0.5em; font-size:30pt !important; text-align: center; width: 900px; margin: 100px auto;">It appears you're using Internet Explorer. This website does not work at his best on older browsers. Please use a modern one like <a href="http://www.google.com/chrome">Chrome</a>, <a href="http://www.mozilla.org/en-US/firefox/new">Firefox</a> or <a href="http://www.apple.com/safari">Safari</a> to enjoy the full experience. </h2>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/CrustAway">Crust Away</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/CrustAway">Crust Away</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/Terminators">Terminator 5</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/Terminators">Terminator 5</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Attributions">Attributions</a></li>
</ul>
</ul>
</div>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/DataPage">Data Page</a></li>
<li class="folder activeLink"><a href="javascript:void(0);">Parts</a>
<li class="folder activeLink"><a href="javascript:void(0);">Parts</a>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Achievements">Achievements</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Achievements">Judging</a></li>
<li class="folder"><a href="javascript:void(0);">Team</a>
<li class="folder"><a href="javascript:void(0);">Team</a>
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<li><a href="https://igem.org/Team.cgi?id=731">Team Profile</a></li>
<li><a href="https://igem.org/Team.cgi?id=731">Team Profile</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Trento">Where We Are</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Trento">Where We Are</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Gallery">Gallery</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Press">Press</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Meetings">Meetings</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Meetings">Meetings</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">Protocols</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">Protocols</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">H<sub>2</sub>S</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Tools">Tools</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">Tools</a></li>
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</ul>
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<li class="folder"><a href="javascript:void(0);">Safety</a>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Safety">Safety</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/H2S">H<sub>2</sub>S</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Safety">Safety</a></li>
 
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Outreach">Outreach</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Outreach">Outreach</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Art">Art&Science</a></li>
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<div class="slider-wrapper theme-default">
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<a href="javascript:void(0);"><img src="http://www.science.unitn.it/~igem/img/slider/Team1.jpg" alt="" /></a>
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<a href="javascript:void(0);"><img src="http://www.science.unitn.it/~igem/img/slider/Parts1.jpg" alt="" /></a>
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<img src="http://www.science.unitn.it/~igem/img/slider/Parts3.jpg" alt="" />
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<img src="http://www.science.unitn.it/~igem/img/slider/Parts4.jpg" alt="" />
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<img src="http://www.science.unitn.it/~igem/img/slider/Parts5.jpg" alt="" />
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<p>Here you can find our favourite Parts and all the Parts we built. Be also sure to check out our <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=UNITN-Trento">Team Parts Page on the Registry website</a>!</p>
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<p>Here you can find our Best Parts and all the Parts we built. Make sure to check out our <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=UNITN-Trento">Team Parts Page on the Registry website</a>!</p>
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<hr style="width: 98%;">
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<h2 style="text-align: center;">BEST PARTS</h2>
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<div class="favPart" style="border-left: 7px solid #50aae4; padding-left: 10px; margin-bottom: 40px;">
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<div class="star"></div>
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">K731030 - Inducible araC-pBAD promoter regulating M256I CysE</a></h2>
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<p>A serine acetyltransferase (CysE) controlled by an arabinose inducible system. <br/>
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This is a key part in our black crust project: a device used to reduce sulfate and produce cysteine. </p>
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<!--<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>-->
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</div>
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<div class="favPart" style="border-left: 7px solid #67f853; padding-left: 10px; margin-bottom: 40px;">
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<div class="star"></div>
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">K731400 - IPTG inducible Cysteine desulfhydrase (CysDes)</a></h2>
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<p>Our favorite BioBrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key part for the Crust Away project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid.</p>
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<!--<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>-->
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</div>
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<div class="favPart" style="border-left: 7px solid #ffae00; padding-left: 10px; margin-bottom: 40px;">
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<div class="star"></div>
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<h2 class="orange"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">K731722 - rrnB T1 terminator</a></h2>
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<p>Our new entry in the terminator collection of the Registry: a functioning version of <a href="http://partsregistry.org/Part:BBa_B0010">BBa_B0010</a>! This terminator was characterized with both T7 and <i>E. coli</i> RNA polymerases. </p>
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<!--<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>-->
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<hr>
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<h2 style="text-align: center;"> All Our Parts</h2>
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<p>Please note that many parts have been also subcloned into low copy vectors. Although these parts have note been submitted to the registry, they are available upon request. Contact us at igemtrento [at] gmail [dot] com</p>
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<h3>The Black Crust Enzymes</h3>
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<div class="Part blue">
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731000">K731000 CysE (Serine Acetyltransferase)</a></h2>
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<p>A Part encoding CysE, our key enzyme in the reduction of sulfate.</p>
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</div>
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<div class="Part blue">
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731010">K731010 M256I CysE (Serine Acetyltransferase)</a></h2>
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<p>This is a mutated CysE. Introduction of a Methionine at position 256, makes our key player even "stronger" and enhances sulfate uptake.
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</p>
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</div>
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<div class="Part blue">
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731020">K731020 Inducible araC-pBAD promoter regulating WT CysE</a></h2>
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<p>A device to produce CysE upon induction with arabinose. Also available in pSB3C5.
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</p>
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</div>
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<div class="Part blue" >
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">K731030 Inducible araC-pBAD promoter regulating M256I CysE</a></h2>
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<p>A device for the production of M256I CysE controlled by an arabinose inducible cassette. This Part was used for the bioremoval of the black crust from different stones samples. Also available in pSB3C5.</p>
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</div>
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<div class="Part blue">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731040">K731040 Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE</a></h2>
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<p>This is our favorite Part <a href="http://partsregistry.org/Part:BBa_K731030">BBa_K731030</a> followed by a sfGFP. Created by Gibson assembly.
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</p>
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</div>
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<div class="Part green" >
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">K731400 IPTG inducible Cysteine desulfhydrase (CysDes)</a></h2>
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<p>Our favorite Bi Bbrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key Part for the Crust Away project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid. Also available in pSB4K5.</p>
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</div>
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<div class="Part green">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731480">K731480 IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)</a></h2>
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<p>This is a sfGFP tagged version of our best BioBrick BBa_K731400. A superfluo device created by Gibson assembly.  Also available in pSB4K5.
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</p>
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</div>
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<h3>Terminators</h3>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731721">K731721 T7 terminator</a></h2>
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<p>Our new entry in the terminator collections of the Registry: a T7 terminator! This terminator was characterized in vivo and in vitro with both T7 and <i>E. coli</i> polymerases. </p>
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</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">K731722 rrnB T1 terminator <i>E. coli</i> terminator</a></h2>
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<p>A good working alternative for the E.coli terminator <a href="http://partsregistry.org/Part:BBa_B0010">BBa_B0010</a>. </p>
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</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">K731700 Platform for terminators analysis under the control of T7 promoter</a></h2>
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<p>Our measurement device for terminators characterization. A T7 promoter controlling the expression of A206K Venus and mCherry for the ratiometric analysis of terminator efficiency.</p>
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</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731710">K731710 Platform for terminators analysis under the control of TAC promoter</a></h2>
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<p>Our great platform <a href="http://partsregistry.org/Part:BBa_K731722">BBa_K731722</a>, where T7 promoter was substituted with Ptac.</p>
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</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731701">K731701 BBa_K731700 with T7terminator (BBa_K731721)</a></h2>
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<p>A measurement device used to characterize T7 terminator under the control of T7 promoter.</p>
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</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731702">K731702 BBa_K731700 with BBa_K731722 rrnB T1 terminator (BBa_B0010)</a></h2>
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<p>A measurement device used to characterize B0010 terminator under the control of T7 promoter.</p>
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</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731711">K731711 BBa_K731710 with T7 terminator (BBa_K731721)</a></h2>
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<p>A measurement device used to characterize the T7 terminator (<a href="http://partsregistry.org/Part:BBa_K731721">BBa_K731721</a>) under the control of tac promoter.</p>
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</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731712">K731712 BBa_K731710 with BBa_K731722 rrnB T1 terminator (<a href="http://partsregistry.org/Part:BBa_B0010">BBa_B0010</a>)</a></h2>
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<p>A measurement device used to characterize the E.coli terminator (<a href="http://partsregistry.org/Part:BBa_K731722">BBa_K731722</a>) under the control of tac promoter.</p>
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</div>
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<h3>Promoters</h3>
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<div class="Part blue">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731201">K731201 Inducible araC-pBAD promoter</a></h2>
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<p>A new version of the araC-pBad promoter that was amplified from the E.coli genome. An excellent alternative to <a href="http://partsregistry.org/Part:BBa_I0050">BBa_I0050</a>.</p>
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</div>
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<div class="Part green">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731300">K731300 LacI + LacIq promoter (reverse)</a></h2>
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<p>A ready to go IPTG inducible cassette that is composed of LacI controlled by the lacIq promoter, both in the reverse direction.</p>
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</div>
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<div class="Part green">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731500">K731500 [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward</a></h2>
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<p>Our Part <a href="http://partsregistry.org/Part:BBa_K731300">BBa_K731300</a> followed by a tac promoter and a lac operator. The perfect BioBrick for those interested in expressing their gene under the control of IPTG. It will save you a lot of cloning steps! Also available in pSB4K5.
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</p>
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</div>
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<div class="Part blue">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731250">K731250 Inducible araC-pBAD promoter with GFPmut3b, strong RBS</a></h2>
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<p>A device for the characterization of our new entry araC-pBad (<a href="http://partsregistry.org/Part:BBa_K731201">BBa_K731201</a>) followed by a strong RBS and a GFPmut3b. Also available in pSB3C5.</p>
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</div>
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<div class="Part blue" >
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731255">K731255 Inducible araC-pBAD promoter with GFPmut3b, weak RBS</a></h2>
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<p>A device for the characterization of our new entry araC-pBad (<a href="http://partsregistry.org/Part:BBa_K731201">BBa_K731201</a>) followed by a weak RBS and GFPmut3b.</p>
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</div>
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<hr style="width: 98%;">
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<div class="Part green">
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731520">K731520 IPTG inducible GFPmut3b</a></h2>
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<h2 style="text-align: center;">FAVORITES</h2>
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<p>A device for the characterization of our new <a href="http://partsregistry.org/Part:BBa_K731500">BBa_K731500</a> followed by a strong RBS and GFPmut3b. Also available in pSB4K5.</p>
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<div class="favPart" style="border-left: 7px solid #50aae4; padding-left: 10px;">
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<div class="star"></div>
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">K731030 - Inducible araC-pBAD promoter regulating M256I CysE</a></h2>
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<p>The CysE M256I gene (K731010) is here regulated by the araC-pBAD promoter (K731201), which is inducible by arabinose. This part was sub cloned and characterized in psB3C5.</p>
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<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>
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<div class="favPart" style="border-left: 7px solid #67f853; padding-left: 10px;">
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">K731400 - IPTG inducible Cysteine desulfhydrase (CysDes)</a></h2>
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<p>This part is composed of K731300 and K731600. It is an IPTG inducible device regulating the expression of a Cysteine Desulfhydrase from Treponema Denticola (CysDes). This enzyme is an aminotransferase that converts cysteine into pyruvate, ammonia and hydrogen sulfide. The part has been successfully operated and characterized in psB1C3 and psB4K5.</p>
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<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>
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<div class="favPart" style="border-left: 7px solid #ffae00; padding-left: 10px;">
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<div class="star"></div>
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731710">K731710 - Platform for terminators analysis with E. coli RNA polymerase by fluorimetric assay</a></h2>
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<p>This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus), separated by Prefix-Suffix linker, allow standardization of terminator's presence effect. The vector was obained by mutagenesis of BBa_K731700, replacing T7 promoter with pTAC. It contains Ampicillin resistance gene and is inducible by IPTG (pTAC-lacO).
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The combined use of BBa_K731710 and BBa_K731700 allow also to analyse any potential difference in terminators' activity due to different polymerases.</p>
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<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>
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<h2 style="text-align: center;"> All our other Parts</h2>
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<h2 class="blue"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731000">K731000 - CysE (Serine Acetyltransferase)</a></h2>
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<p>CysE is an enzyme involved in cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, a precursor for the biosynthesis of cysteine. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes.</p>
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<div class="Part">
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<h2 class="blue"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731010">K731010 - M256I CysE (Serine Acetyltransferase)</a></h2>
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<p>CysE is an enzyme involved In cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, the CysE final precursor. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes.
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M256I CysE shows enhanced secretion of cysteine and is not inhibited by Cysteine overproduction.</p>
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</div> <div class="clearfix"></div>
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<div class="Part">
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<h2 class="blue"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731020">K731020 - araC-pBAD regulated WT CysE</a></h2>
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<p>The CysE gene (K731000) is here regulated by the araC-pBAD promoter (K731201), which is inducible by arabinose. This part was sub cloned and characterized in psB3C5.</p>
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</div>
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<div class="Part">
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<h2 class="blue"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731040">K731040 - araC-pBAD regulated sfGFP-tagged M256I CysE</a></h2>
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<p>A sfGFP tagged M256I CysE inducible by arabinose. This part was subcloned and characterized in pSB3C5.</p>
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</div><div class="clearfix"></div>
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<div class="Part">
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<h2 class="blue"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731201">K731201 - Inducible araC-pBAD promoter</a></h2>
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<p>This E. coli promoter is positively regulated by arabinose.
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araC is the regulator protein: in absence of arabinose the protein binds to araI1 and araO2 and the DNA forms a loop that prevents RNA polymerase from binding the promoter. When arabinose is added to the culture it binds to araC, disabling the interaction with araI1 and araO2 sites, and also allowing it to bind araI site, promoting transcription.
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Promoter activity is also influenced by glucose levels: in its absence cAMP levels are high and the the complex cAMP+CAP (catabolite activator protein) binds to lacI promoting transcription. In presence of glucose, cAMP levels are low and promoter activity is inhibited.</p>
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</div>
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<div class="Part">
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<h2 class="blue"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731250">K731250 - Inducible araC-pBAD promoter with GFPmut3b, strong RBS</a></h2>
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<p>This part has been used for testing and characterization of araC-pBAD promoter (BBa_K731201). The part is composed of part BBa_K731201 and BBa_E0840.
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This part was successfully operated and characterized both in psB1C3 and psB3C5.</p>
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</div><div class="clearfix"></div>
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<div class="Part">
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<h2 class="blue"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731255">K731255 - Inducible araC-pBAD promoter with GFPmut3b, weak RBS</a></h2>
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<p>This part has been used for testing and characterization of araC-pBAD promoter (part BBa_K731200) when using a weak RBS. The part is composed of part BBa_K731200 and BBa_E0240.</p>
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</div>
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<div class="Part">
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<h2 class="green"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731300">K731300 - LacI + LacIq promoter (reverse)</a></h2>
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<p>This transcriptional regulator includes the LacI repressor and its constitutive promoter LacIq which operates in the reverse direction. The LacI repressor protein is inhibited by IPTG, and it is a very useful device to control and regulate the expression of a gene of interest.</p>
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</div><div class="clearfix"></div>
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<div class="Part">
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<h2 class="green"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">K731400 - IPTG inducible Cysteine desulfhydrase (CysDes)</a></h2>
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<p>This part is composed of K731300 and K731600. It is an IPTG inducible device regulating the expression of a Cysteine Desulfhydrase from Treponema Denticola (CysDes). This enzyme is an aminotransferase that converts cysteine into pyruvate, ammonia and hydrogen sulfide. The part has been successfully operated and characterized in psB1C3 and psB4K5.</p>
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</div>
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<div class="Part">
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<h2 class="green"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731500">K731500 - Inducibile tac promoter and lac operon</a></h2>
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<p>This is E.coli lactose-inducibile promoter.
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lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription. Low, constitutive levels of transcription can be seen anyhow.</p>
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</div><div class="clearfix"></div>
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<div class="Part">
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<h2 class="green"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731520">K731520 - Lactose-inducible sfGFP</a></h2>
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<p>This is a construct carrying the sfGFP gene with low-efficiency RBS, under lac operator control.
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lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.Low, constitutive levels of transcription can be seen anyhow.</p>
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</div>
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<div class="Part">
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<h2 class="green"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731600">K731600 - Cysteine Desulfhydrase (CysDes)</a></h2>
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<p>This part encode for a cysteine desulfhydrase from Treponema denticola, which is known to be a strong enzyme that produces H2S, ammonia and pyruvate starting from cysteine. It is often indicated also as Hly.</p>
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<div class="Part">
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<h2 class="orange"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">K731700 - T7 Platform</a></h2>
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<p>This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus), separated by Prefix-Suffix linker, allow standardization of terminator's presence effect. The vector is a modified pET21b; it contains Ampicillin resistance gene and is inducible by IPTG (T7promoter-lacO).
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The combined use of BBa_K731710 and BBa_K731700 allow also to analyse any potential difference in terminators' activity due to different polymerases.</p>
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</div>
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<div class="Part">
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<h2 class="orange"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731701">K731701 - BBa_K731700 with T7 terminator</a></h2>
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<p>T7 terminator T7 polymerase testing construct.</p>
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<h2 class="orange"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731702">K731702 - BBa_K731700 with BBa_K731722 terminator</a></h2>
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<p>E. Coli terminator T7 polymerase testing construct.</p>
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<h2 class="orange"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731">K731711 - BBa_K731710 with T7 terminator</a></h2>
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<p>T7 terminator coli polymerase testing construct.</p>
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</div><div class="clearfix"></div>
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<div class="Part">
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<h2 class="orange"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731712">K731712 - BBa_K731710 with BBa_K731722 terminator</a></h2>
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<p>E. coli terminator coli polymerase testing construct 2.</p>
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<h2 class="orange"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731712">K731712 - BBa_K731710 with BBa_K731722 terminator</a></h2>
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<p>E. coli terminator coli polymerase testing construct 2.</p>
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<h2 class="orange"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731721">K731721 - T7 terminator</a></h2>
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<p>Gag ipsum dolar sit amet venenatis charlie sheen lose i'm watching u. Female weekend finals close enough derp top note so close architect yao. Face oboma unlock ipsum t-rex keyboard just simpson gag elephant bacon cuteness overload. </p>
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<h2 class="orange"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">K731722 - B0010 Coli terminator</a></h2>
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<p>Gag ipsum dolar sit amet venenatis charlie sheen lose i'm watching u. Female weekend finals close enough derp top note so close architect yao. Face oboma unlock ipsum t-rex keyboard just simpson gag elephant bacon cuteness overload. </p>
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Latest revision as of 17:34, 25 September 2012

Our Parts

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Our Parts

Here you can find our Best Parts and all the Parts we built. Make sure to check out our Team Parts Page on the Registry website!

BEST PARTS

K731030 - Inducible araC-pBAD promoter regulating M256I CysE

A serine acetyltransferase (CysE) controlled by an arabinose inducible system.
This is a key part in our black crust project: a device used to reduce sulfate and produce cysteine.

K731400 - IPTG inducible Cysteine desulfhydrase (CysDes)

Our favorite BioBrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key part for the Crust Away project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid.

K731722 - rrnB T1 terminator

Our new entry in the terminator collection of the Registry: a functioning version of BBa_B0010! This terminator was characterized with both T7 and E. coli RNA polymerases.

All Our Parts

Please note that many parts have been also subcloned into low copy vectors. Although these parts have note been submitted to the registry, they are available upon request. Contact us at igemtrento [at] gmail [dot] com

The Black Crust Enzymes

K731000 CysE (Serine Acetyltransferase)

A Part encoding CysE, our key enzyme in the reduction of sulfate.

K731010 M256I CysE (Serine Acetyltransferase)

This is a mutated CysE. Introduction of a Methionine at position 256, makes our key player even "stronger" and enhances sulfate uptake.

K731020 Inducible araC-pBAD promoter regulating WT CysE

A device to produce CysE upon induction with arabinose. Also available in pSB3C5.

K731030 Inducible araC-pBAD promoter regulating M256I CysE

A device for the production of M256I CysE controlled by an arabinose inducible cassette. This Part was used for the bioremoval of the black crust from different stones samples. Also available in pSB3C5.

K731040 Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE

This is our favorite Part BBa_K731030 followed by a sfGFP. Created by Gibson assembly.

K731400 IPTG inducible Cysteine desulfhydrase (CysDes)

Our favorite Bi Bbrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key Part for the Crust Away project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid. Also available in pSB4K5.

K731480 IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)

This is a sfGFP tagged version of our best BioBrick BBa_K731400. A superfluo device created by Gibson assembly. Also available in pSB4K5.

Terminators

K731721 T7 terminator

Our new entry in the terminator collections of the Registry: a T7 terminator! This terminator was characterized in vivo and in vitro with both T7 and E. coli polymerases.

K731722 rrnB T1 terminator E. coli terminator

A good working alternative for the E.coli terminator BBa_B0010.

K731700 Platform for terminators analysis under the control of T7 promoter

Our measurement device for terminators characterization. A T7 promoter controlling the expression of A206K Venus and mCherry for the ratiometric analysis of terminator efficiency.

K731710 Platform for terminators analysis under the control of TAC promoter

Our great platform BBa_K731722, where T7 promoter was substituted with Ptac.

K731701 BBa_K731700 with T7terminator (BBa_K731721)

A measurement device used to characterize T7 terminator under the control of T7 promoter.

K731702 BBa_K731700 with BBa_K731722 rrnB T1 terminator (BBa_B0010)

A measurement device used to characterize B0010 terminator under the control of T7 promoter.

K731711 BBa_K731710 with T7 terminator (BBa_K731721)

A measurement device used to characterize the T7 terminator (BBa_K731721) under the control of tac promoter.

K731712 BBa_K731710 with BBa_K731722 rrnB T1 terminator (BBa_B0010)

A measurement device used to characterize the E.coli terminator (BBa_K731722) under the control of tac promoter.

Promoters

K731201 Inducible araC-pBAD promoter

A new version of the araC-pBad promoter that was amplified from the E.coli genome. An excellent alternative to BBa_I0050.

K731300 LacI + LacIq promoter (reverse)

A ready to go IPTG inducible cassette that is composed of LacI controlled by the lacIq promoter, both in the reverse direction.

K731500 [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward

Our Part BBa_K731300 followed by a tac promoter and a lac operator. The perfect BioBrick for those interested in expressing their gene under the control of IPTG. It will save you a lot of cloning steps! Also available in pSB4K5.

K731250 Inducible araC-pBAD promoter with GFPmut3b, strong RBS

A device for the characterization of our new entry araC-pBad (BBa_K731201) followed by a strong RBS and a GFPmut3b. Also available in pSB3C5.

K731255 Inducible araC-pBAD promoter with GFPmut3b, weak RBS

A device for the characterization of our new entry araC-pBad (BBa_K731201) followed by a weak RBS and GFPmut3b.

K731520 IPTG inducible GFPmut3b

A device for the characterization of our new BBa_K731500 followed by a strong RBS and GFPmut3b. Also available in pSB4K5.

Retrieved from "http://2012.igem.org/Team:UNITN-Trento/Parts"