Team:UNITN-Trento/Parts

From 2012.igem.org

(Difference between revisions)
 
(23 intermediate revisions not shown)
Line 2: Line 2:
<head>
<head>
    
    
-
+
<meta name="viewport" content="width=1200, user-scalable=0">
<meta charset="utf-8" />
<meta charset="utf-8" />
<title>Our Parts</title>
<title>Our Parts</title>
Line 8: Line 8:
<link rel="stylesheet" type="text/css" href="http://fonts.googleapis.com/css?family=Open+Sans:300,300italic,400,400italic,600,600italic,700,700italic,800,800italic|Bree+Serif:400|Arizonia:400|Open+Sans:300,400,600,700,800,300italic,400italic,600italic,700italic,800italic"/>
<link rel="stylesheet" type="text/css" href="http://fonts.googleapis.com/css?family=Open+Sans:300,300italic,400,400italic,600,600italic,700,700italic,800,800italic|Bree+Serif:400|Arizonia:400|Open+Sans:300,400,600,700,800,300italic,400italic,600italic,700italic,800italic"/>
-
<link rel="stylesheet" type="text/css" href="http://www.jasonfweb.com/igem2/css/style.css"/>
+
<link rel="stylesheet" type="text/css" href="http://www.science.unitn.it/~igem/css/style.css"/>
-
<link rel="stylesheet" type="text/css" href="http://www.jasonfweb.com/igem2/css/override.css"/>
+
<link rel="stylesheet" type="text/css" href="http://www.science.unitn.it/~igem/css/override.css"/>
-
<link rel="stylesheet" type="text/css" href="http://www.jasonfweb.com/igem2/css/background/kit.css"/>
+
<link rel="stylesheet" type="text/css" href="http://www.science.unitn.it/~igem/css/background/kit.css"/>
<script src="https://ajax.googleapis.com/ajax/libs/jquery/1.7.1/jquery.min.js"></script>
<script src="https://ajax.googleapis.com/ajax/libs/jquery/1.7.1/jquery.min.js"></script>
-
<link rel="stylesheet" href="http://www.jasonfweb.com/igem2/js/nivo/nivo-slider.css" type="text/css" media="screen"/>
+
<link rel="stylesheet" href="http://www.science.unitn.it/~igem/js/nivo/nivo-slider.css" type="text/css" media="screen"/>
-
<link rel="stylesheet" href="http://www.jasonfweb.com/igem2/js/nivo/themes/default/default.css" type="text/css" media="screen"/>
+
<link rel="stylesheet" href="http://www.science.unitn.it/~igem/js/nivo/themes/default/default.css" type="text/css" media="screen"/>
<script src="https://ajax.googleapis.com/ajax/libs/jquery/1.7.1/jquery.min.js"></script>
<script src="https://ajax.googleapis.com/ajax/libs/jquery/1.7.1/jquery.min.js"></script>
-
<script src="http://www.jasonfweb.com/igem2/js/nivo/jquery.nivo.slider.pack.js" type="text/javascript"></script>
+
<script src="http://www.science.unitn.it/~igem/js/nivo/jquery.nivo.slider.pack.js" type="text/javascript"></script>
<script>
<script>
jQuery(function($){
jQuery(function($){
Line 31: Line 31:
<body>
<body>
 +
 +
<!--[if lte IE 9]>
 +
<style type="text/css">
 +
body {
 +
filter:blur(add=false, direction=270 strength=10);
 +
-ms-filter:blur(add=false, direction=270 strength=10);
 +
background-image: none;
 +
}
 +
#bannerImage {
 +
display: none;
 +
}
 +
#header {
 +
display: none;
 +
}
 +
#canvas {
 +
display: none;
 +
}
 +
#footer {
 +
display: none;
 +
}
 +
</style>
 +
<h2 style="font-family: 'Open Sans'; line-height: 40px; color:#ffae00; padding:0.5em; font-size:30pt !important; text-align: center; width: 900px; margin: 100px auto;">It appears you're using Internet Explorer. This website does not work at his best on older browsers. Please use a modern one like <a href="http://www.google.com/chrome">Chrome</a>, <a href="http://www.mozilla.org/en-US/firefox/new">Firefox</a> or <a href="http://www.apple.com/safari">Safari</a> to enjoy the full experience. </h2>
 +
<![endif]-->
 +
 +
<noscript>
 +
<style type="text/css">
 +
body {
 +
filter:blur(add=false, direction=270 strength=10);
 +
-ms-filter:blur(add=false, direction=270 strength=10);
 +
background-image: none;
 +
}
 +
#bannerImage {
 +
display: none;
 +
}
 +
#header {
 +
display: none;
 +
}
 +
#canvas {
 +
display: none;
 +
}
 +
 +
#footer {
 +
display: none;
 +
}
 +
</style>
 +
 +
<h2 style="font-family: 'Open Sans'; line-height: 40px; color:#ffae00; padding:0.5em; font-size:30pt !important; text-align: center; width: 900px; margin: 100px auto;">It appears you have JavaScript disabled. Our Wiki needs it for you to enjoy the full experience. Please enable Javascript and refresh the page.</h2>
 +
</noscript>
<div id="bannerImage" class="content-fill" style="overflow: hidden; position: absolute; height: 400px; background-position: 50% 58%;"></div>
<div id="bannerImage" class="content-fill" style="overflow: hidden; position: absolute; height: 400px; background-position: 50% 58%;"></div>
Line 43: Line 91:
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/CrustAway">Crust Away</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/CrustAway">Crust Away</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/Terminators">Terminator 5</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project/Terminators">Terminator 5</a></li>
 +
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Attributions">Attributions</a></li>
</ul>
</ul>
</div>
</div>
</li>
</li>
 +
<li><a href="https://2012.igem.org/Team:UNITN-Trento/DataPage">Data Page</a></li>
<li class="folder activeLink"><a href="javascript:void(0);">Parts</a>
<li class="folder activeLink"><a href="javascript:void(0);">Parts</a>
<div class="notch"></div>
<div class="notch"></div>
Line 55: Line 105:
</div>
</div>
</li>
</li>
-
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Achievements">Achievements</a></li>
+
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Achievements">Judging</a></li>
<li class="folder"><a href="javascript:void(0);">Team</a>
<li class="folder"><a href="javascript:void(0);">Team</a>
<div class="notch"></div>
<div class="notch"></div>
Line 63: Line 113:
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Team/Supervisors">Super- & Ad- visors</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Team/Supervisors">Super- & Ad- visors</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Friends">Our Friends</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Friends">Our Friends</a></li>
-
<li><a href="javascript:void(0);">Team Profile</a></li>
+
<li><a href="https://igem.org/Team.cgi?id=731">Team Profile</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Trento">Where We Are</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Trento">Where We Are</a></li>
-
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Gallery">Gallery</a></li>
+
 +
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Press">Press</a></li>
</ul>
</ul>
</div>
</div>
Line 84: Line 135:
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Meetings">Meetings</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Meetings">Meetings</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">Protocols</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">Protocols</a></li>
-
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">H<sub>2</sub>S</a></li>
+
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Tools">Tools</a></li>
-
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">Tools</a></li>
+
</ul>
 +
</div>
 +
</li>
 +
<li class="folder"><a href="javascript:void(0);">Safety</a>
 +
<div class="notch"></div>
 +
<div class="subnav" >
 +
<ul>
 +
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Safety">Safety</a></li>
 +
<li><a href="https://2012.igem.org/Team:UNITN-Trento/H2S">H<sub>2</sub>S</a></li>
</ul>
</ul>
</div>
</div>
</li>
</li>
-
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Safety">Safety</a></li>
 
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Outreach">Outreach</a></li>
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Outreach">Outreach</a></li>
 +
<li><a href="https://2012.igem.org/Team:UNITN-Trento/Art">Art&Science</a></li>
</ul>
</ul>
</div>
</div>
<div id="logo">
<div id="logo">
      <div class="wrapper">
      <div class="wrapper">
-
      <h1><a href="javascript:void(0);" style="font-family: 'Bree Serif';">iGEM Trento 2012</a></h1>
+
      <h1><a href="https://2012.igem.org/Team:UNITN-Trento" style="font-family: 'Bree Serif';"><span>i</span>GEM Trento 2012</a></h1>
      </div>
      </div>
</div>
</div>
Line 106: Line 165:
<section id="main" style="width:100%; position: relative;">
<section id="main" style="width:100%; position: relative;">
-
<div id="igem" style="position: absolute; right: 25px; top: 205px; width: 80px; z-index: 10;"><a href="https://igem.org/Main_Page"><img src="http://www.jasonfweb.com/igem2/img/sponsor/iGEM.png" width="75px" alt="" /></a></div>
+
<div id="igem" style="position: absolute; right: 25px; top: 205px; width: 80px; z-index: 10;"><a href="https://igem.org/Main_Page"><img src="http://www.science.unitn.it/~igem/img/sponsor/iGEM.png" width="75px" alt="" /></a></div>
<div class="slider-wrapper theme-default">
<div class="slider-wrapper theme-default">
<div id="slider" class="nivoSlider">
<div id="slider" class="nivoSlider">
-
<a href="javascript:void(0);"><img src="http://www.jasonfweb.com/igem2/img/team-1.jpg" alt="" /></a>
+
<a href="javascript:void(0);"><img src="http://www.science.unitn.it/~igem/img/slider/Parts1.jpg" alt="" /></a>
-
<img src="http://www.jasonfweb.com/igem2/img/team-2.jpg" alt="" />
+
<img src="http://www.science.unitn.it/~igem/img/slider/Parts2.jpg" alt="" />
-
<img src="http://www.jasonfweb.com/igem2/img/team-3.jpg" alt="" />
+
<img src="http://www.science.unitn.it/~igem/img/slider/Home6.jpg" alt="" />
-
<img src="http://www.jasonfweb.com/igem2/img/team-4.jpg" alt="" />
+
<img src="http://www.science.unitn.it/~igem/img/slider/Parts3.jpg" alt="" />
 +
<img src="http://www.science.unitn.it/~igem/img/slider/Parts4.jpg" alt="" />
 +
<img src="http://www.science.unitn.it/~igem/img/slider/Parts5.jpg" alt="" />
 +
<img src="http://www.science.unitn.it/~igem/img/slider/Parts6.jpg" alt="" />
</div>
</div>
</div>
</div>
<div class="punch" style="margin: 0 auto;">
<div class="punch" style="margin: 0 auto;">
-
<h2 style="text-align: center;">Gallery</h2>
+
<h2 style="text-align: center;">Our Parts</h2>
</div>
</div>
<hr style="width: 100%;">
<hr style="width: 100%;">
Line 125: Line 187:
-
<h2>Our Parts</h2>
+
<p>Here you can find our Best Parts and all the Parts we built. Make sure to check out our <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=UNITN-Trento">Team Parts Page on the Registry website</a>!</p>
-
<p>Here you can find all the Parts we built. Be sure to check out our <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=UNITN-Trento">Team Parts Page on the Registry website</a>!</p>
+
-
+
<hr style="width: 98%;">
-
<table class="pgrouptable addborder" style="width: 925px;" cellpadding="0" cellspacing="0"><tbody><tr><th style="width: 7em;">Name</th><th colspan="">Type</th><th colspan="">Description</th><th colspan="">Designer</th><th class="blank_col">
+
-
+
<h2 style="text-align: center;">BEST PARTS</h2>
-
<!-- ROW -->
+
-
+
<div class="favPart" style="border-left: 7px solid #50aae4; padding-left: 10px; margin-bottom: 40px;">
-
<!-- ROW -->
+
<div class="star"></div>
-
+
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">K731030 - Inducible araC-pBAD promoter regulating M256I CysE</a></h2>
-
<!-- ROW -->
+
<p>A serine acetyltransferase (CysE) controlled by an arabinose inducible system. <br/>
-
+
This is a key part in our black crust project: a device used to reduce sulfate and produce cysteine. </p>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data">
+
<!--<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>-->
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25292"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></form></th></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731000">BBa_K731000</a></td><td>Coding</td><td>CysE (Serine Acetyltransferase)</td><td >Jason Fontana, Daniele Rossetto, Giacomo Giacomelli</td>
+
</div>
 +
 +
<div class="favPart" style="border-left: 7px solid #67f853; padding-left: 10px; margin-bottom: 40px;">
 +
<div class="star"></div>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">K731400 - IPTG inducible Cysteine desulfhydrase (CysDes)</a></h2>
 +
<p>Our favorite BioBrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key part for the Crust Away project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid.</p>
 +
<!--<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>-->
 +
</div>
 +
 +
<div class="favPart" style="border-left: 7px solid #ffae00; padding-left: 10px; margin-bottom: 40px;">
 +
<div class="star"></div>
 +
<h2 class="orange"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">K731722 - rrnB T1 terminator</a></h2>
 +
<p>Our new entry in the terminator collection of the Registry: a functioning version of <a href="http://partsregistry.org/Part:BBa_B0010">BBa_B0010</a>! This terminator was characterized with both T7 and <i>E. coli</i> RNA polymerases. </p>
 +
<!--<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>-->
 +
</div>
 +
 +
 +
 +
 +
<hr>
 +
 +
<h2 style="text-align: center;"> All Our Parts</h2>
 +
 +
<p>Please note that many parts have been also subcloned into low copy vectors. Although these parts have note been submitted to the registry, they are available upon request. Contact us at igemtrento [at] gmail [dot] com</p>
 +
 +
<h3>The Black Crust Enzymes</h3>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<div class="Part blue">
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25430"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731010">BBa_K731010</a></td><td>Coding</td><td>M256I CysE (Serine Acetyltransferase)</td><td >Jason Fontana, Daniele Rossetto, Giacomo Giacomelli</td>
+
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731000">K731000 CysE (Serine Acetyltransferase)</a></h2>
-
+
<p>A Part encoding CysE, our key enzyme in the reduction of sulfate.</p>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
</div>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25431"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731020">BBa_K731020</a></td><td>Composite</td><td>Inducible araC-pBAD promoter regulating WT CysE</td><td >Jason Fontana</td>
+
-
+
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<div class="Part blue">
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25432"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">BBa_K731030</a></td><td>Composite</td><td>Inducible araC-pBAD promoter regulating M256I CysE</td><td >Jason Fontana, Daniele Rossetto</td>
+
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731010">K731010 M256I CysE (Serine Acetyltransferase)</a></h2>
-
+
<p>This is a mutated CysE. Introduction of a Methionine at position 256, makes our key player even "stronger" and enhances sulfate uptake.
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
</p>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25433"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731040">BBa_K731040</a></td><td>Composite</td><td>Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE</td><td >Jason Fontana, Daniele Rossetto</td>
+
</div>
-
+
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<div class="Part blue">
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25355"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731201">BBa_K731201</a></td><td>Regulatory</td><td>Inducible araC-pBAD promoter</td><td >Daniele Rossetto</td>
+
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731020">K731020 Inducible araC-pBAD promoter regulating WT CysE</a></h2>
-
+
<p>A device to produce CysE upon induction with arabinose. Also available in pSB3C5.
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
</p>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25356"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731250">BBa_K731250</a></td><td>Composite</td><td>Inducible araC-pBAD promoter with GFPmut3b, strong RBS</td><td >Daniele Rossetto</td>
+
</div>
-
+
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<div class="Part blue" >
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25357"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731255">BBa_K731255</a></td><td>Composite</td><td>Inducible araC-pBAD promoter with GFPmut3b, weak RBS</td><td >Daniele Rossetto</td>
+
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">K731030 Inducible araC-pBAD promoter regulating M256I CysE</a></h2>
-
+
<p>A device for the production of M256I CysE controlled by an arabinose inducible cassette. This Part was used for the bioremoval of the black crust from different stones samples. Also available in pSB3C5.</p>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
</div>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25439"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731300">BBa_K731300</a></td><td>Regulatory</td><td>LacI + LacIq promoter (reverse)</td><td >Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli</td>
+
-
+
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<div class="Part blue">
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25440"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">BBa_K731400</a></td><td>Composite</td><td>IPTG inducible Cysteine desulfhydrase (CysDes)</td><td >Andrea Tassinari</td>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731040">K731040 Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE</a></h2>
-
+
<p>This is our favorite Part <a href="http://partsregistry.org/Part:BBa_K731030">BBa_K731030</a> followed by a sfGFP. Created by Gibson assembly.
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
</p>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25444"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731480">BBa_K731480</a></td><td>Composite</td><td>IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)</td><td >Andrea Tassinari, Francesco Guzzonato</td>
+
-
+
</div>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25446"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731500">BBa_K731500</a></td><td>Regulatory</td><td>Inducibile tac promoter and lac operon</td><td >Francesco Guzzonato</td>
+
-
+
<div class="Part green" >
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">K731400 IPTG inducible Cysteine desulfhydrase (CysDes)</a></h2>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25447"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731520">BBa_K731520</a></td><td>Composite</td><td>lactose-inducible sfGFP</td><td >Francesco Guzzonato</td>
+
<p>Our favorite Bi Bbrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key Part for the Crust Away project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid. Also available in pSB4K5.</p>
-
+
</div>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25452"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731600">BBa_K731600</a></td><td>Coding</td><td>Cysteine Desulfhydrase (CysDes)</td><td >Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli</td>
+
<div class="Part green">
-
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731480">K731480 IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)</a></h2>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<p>This is a sfGFP tagged version of our best BioBrick BBa_K731400. A superfluo device created by Gibson assembly.  Also available in pSB4K5.
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25409"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">BBa_K731700</a></td><td>Plasmid_Backbone</td><td>Low copy vector for terminators analysis with T7 RNApolymerase by fluorimetric assay</td><td >Giacomo Giacomelli, Anna Depetris</td>
+
</p>
-
+
</div>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25465"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731701">BBa_K731701</a></td><td>Measurement</td><td>BBa_K731700 with T7terminator (BBa_K731721)</td><td >Giacomo Giacomelli, Anna Depetris</td>
+
-
+
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<h3>Terminators</h3>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25466"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731702">BBa_K731702</a></td><td>Measurement</td><td>BBa_K731700 with BBa_K731722 terminator (BBa_B0010)</td><td >Giacomo Giacomelli, Anna Depetris</td>
+
-
+
<div class="Part orange">
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731721">K731721 T7 terminator</a></h2>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25411"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731710">BBa_K731710</a></td><td>Plasmid_Backbone</td><td>Low copy vector for terminators analysis with Coli RNApolymerase by fluorimetric assay</td><td >Giacomo Giacomelli, Anna Depetris</td>
+
<p>Our new entry in the terminator collections of the Registry: a T7 terminator! This terminator was characterized in vivo and in vitro with both T7 and <i>E. coli</i> polymerases. </p>
-
+
</div>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25464"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731711">BBa_K731711</a></td><td>Measurement</td><td>BBa_K731710 with T7terminator (BBa_K731721)</td><td >Giacomo Giacomelli, Anna Depetris</td>
+
<div class="Part orange">
-
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">K731722 rrnB T1 terminator <i>E. coli</i> terminator</a></h2>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<p>A good working alternative for the E.coli terminator <a href="http://partsregistry.org/Part:BBa_B0010">BBa_B0010</a>. </p>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25462"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731712">BBa_K731712</a></td><td>Measurement</td><td>BBa_K731710 with BBa_K731722 terminator (BBa_B0010)</td><td >Giacomo Giacomelli, Anna Depetris</td>
+
</div>  
-
+
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
<div class="Part orange">
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25413"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731721">BBa_K731721</a></td><td>Terminator</td><td>T7 terminator</td><td >Anna Depetris, Giacomo Giacomelli</td>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">K731700 Platform for terminators analysis under the control of T7 promoter</a></h2>
-
+
<p>Our measurement device for terminators characterization. A T7 promoter controlling the expression of A206K Venus and mCherry for the ratiometric analysis of terminator efficiency.</p>
-
<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
+
</div>
-
<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25455"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">BBa_K731722</a></td><td>Terminator</td><td>B0010 E. coli terminator</td><td >Anna Depetris, Giacomo Giacomelli</td>
+
-
+
<div class="Part orange">
-
</tr></tbody></table>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731710">K731710 Platform for terminators analysis under the control of TAC promoter</a></h2>
-
+
<p>Our great platform <a href="http://partsregistry.org/Part:BBa_K731722">BBa_K731722</a>, where T7 promoter was substituted with Ptac.</p>
-
<!--<div class="member">
+
</div>
-
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
+
-
<div class=partDescription>
+
<div class="Part orange">
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731000">K731000 - CysE (Serine Acetyltransferase)</a></h2>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731701">K731701 BBa_K731700 with T7terminator (BBa_K731721)</a></h2>
-
<p>CysE is an enzyme involved in cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, a precursor for the biosynthesis of cysteine. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes.</p>
+
<p>A measurement device used to characterize T7 terminator under the control of T7 promoter.</p>
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731000">Registry Page</a>
+
</div>
-
</div>
+
-
</div>
+
<div class="Part orange">
-
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731702">K731702 BBa_K731700 with BBa_K731722 rrnB T1 terminator (BBa_B0010)</a></h2>
-
<div class="member bitleft">
+
<p>A measurement device used to characterize B0010 terminator under the control of T7 promoter.</p>
-
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
+
</div>
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731010">K731010 - M256I CysE (Serine Acetyltransferase)</a></h2>
+
<div class="Part orange">
-
<p>CysE is an enzyme involved In cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, the CysE final precursor. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes.
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731711">K731711 BBa_K731710 with T7 terminator (BBa_K731721)</a></h2>
-
M256I CysE shows enhanced secretion of cysteine and is not inhibited by Cysteine overproduction.</p>
+
<p>A measurement device used to characterize the T7 terminator (<a href="http://partsregistry.org/Part:BBa_K731721">BBa_K731721</a>) under the control of tac promoter.</p>
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731010">Registry Page</a>
+
</div>
-
</div>
+
-
</div>
+
<div class="Part orange">
-
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731712">K731712 BBa_K731710 with BBa_K731722 rrnB T1 terminator (<a href="http://partsregistry.org/Part:BBa_B0010">BBa_B0010</a>)</a></h2>
-
<div class="member">
+
<p>A measurement device used to characterize the E.coli terminator (<a href="http://partsregistry.org/Part:BBa_K731722">BBa_K731722</a>) under the control of tac promoter.</p>
-
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
+
</div>
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731020">K731020 - Inducible araC-pBAD promoter regulating WT CysE</a></h2>
+
-
<p>The CysE gene (K731000) is here regulated by the araC-pBAD promoter (K731201), which is inducible by arabinose. This part was sub cloned and characterized in psB3C5.</p>
+
<h3>Promoters</h3>
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731020">Registry Page</a>
+
-
</div>
+
<div class="Part blue">
-
</div>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731201">K731201 Inducible araC-pBAD promoter</a></h2>
-
+
<p>A new version of the araC-pBad promoter that was amplified from the E.coli genome. An excellent alternative to <a href="http://partsregistry.org/Part:BBa_I0050">BBa_I0050</a>.</p>
-
<div class="member bitleft">
+
</div>
-
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
+
-
<div class=partDescription>
+
<div class="Part green">
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">K731030 - Inducible araC-pBAD promoter regulating M256I CysE</a></h2>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731300">K731300 LacI + LacIq promoter (reverse)</a></h2>
-
<p>The CysE M256I gene (K731010) is here regulated by the araC-pBAD promoter (K731201), which is inducible by arabinose. This part was sub cloned and characterized in psB3C5.</p>
+
<p>A ready to go IPTG inducible cassette that is composed of LacI controlled by the lacIq promoter, both in the reverse direction.</p>
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">Registry Page</a>
+
</div>
-
</div>
+
-
</div>
+
<div class="Part green">
-
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731500">K731500 [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward</a></h2>
-
<div class="member">
+
<p>Our Part <a href="http://partsregistry.org/Part:BBa_K731300">BBa_K731300</a> followed by a tac promoter and a lac operator. The perfect BioBrick for those interested in expressing their gene under the control of IPTG. It will save you a lot of cloning steps! Also available in pSB4K5.
-
<img src="http://www.jasonfweb.com/igem2/img/team/Ross.jpg" alt="" />
+
</p>
-
<div class=partDescription>
+
</div>
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731040">K731040 - Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE</a></h2>
+
-
<p>A sfGFP tagged M256I CysE inducible by arabinose. This part was subcloned and characterized in pSB3C5.</p>
+
<div class="Part blue">
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731040">Registry Page</a>
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731250">K731250 Inducible araC-pBAD promoter with GFPmut3b, strong RBS</a></h2>
-
</div>
+
<p>A device for the characterization of our new entry araC-pBad (<a href="http://partsregistry.org/Part:BBa_K731201">BBa_K731201</a>) followed by a strong RBS and a GFPmut3b. Also available in pSB3C5.</p>
-
</div>
+
</div>
-
+
-
<div class="member bitleft">
+
<div class="Part blue" >
-
<img src="http://www.jasonfweb.com/igem2/img/team/Ross.jpg" alt="" />
+
<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731255">K731255 Inducible araC-pBAD promoter with GFPmut3b, weak RBS</a></h2>
-
<div class=partDescription>
+
<p>A device for the characterization of our new entry araC-pBad (<a href="http://partsregistry.org/Part:BBa_K731201">BBa_K731201</a>) followed by a weak RBS and GFPmut3b.</p>
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731201">K731201 - Inducible araC-pBAD promoter</a></h2>
+
</div>
-
<p style="font-size: 11px;">This E. coli promoter is positively regulated by arabinose.
+
-
araC is the regulator protein: in absence of arabinose the protein binds to araI1 and araO2 and the DNA forms a loop that prevents RNA polymerase from binding the promoter. When arabinose is added to the culture it binds to araC, disabling the interaction with araI1 and araO2 sites, and also allowing it to bind araI site, promoting transcription.
+
-
Promoter activity is also influenced by glucose levels: in its absence cAMP levels are high and the the complex cAMP+CAP (catabolite activator protein) binds to lacI promoting transcription. In presence of glucose, cAMP levels are low and promoter activity is inhibited.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731201">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Ross.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731250">K731250 - Inducible araC-pBAD promoter with GFPmut3b, strong RBS</a></h2>
+
-
<p>This part has been used for testing and characterization of araC-pBAD promoter (BBa_K731201). The part is composed of part BBa_K731201 and BBa_E0840.
+
-
This part was successfully operated and characterized both in psB1C3 and psB3C5.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731250">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member bitleft">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Ross.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731255">K731255 - Inducible araC-pBAD promoter with GFPmut3b, weak RBS</a></h2>
+
-
<p>This part has been used for testing and characterization of araC-pBAD promoter (part BBa_K731200) when using a weak RBS. The part is composed of part BBa_K731200 and BBa_E0240.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731255">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Andrea.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731300">K731300 - LacI + LacIq promoter (reverse)</a></h2>
+
-
<p>This transcriptional regulator includes the LacI repressor and its constitutive promoter LacIq which operates in the reverse direction. The LacI repressor protein is inhibited by IPTG, and it is a very useful device to control and regulate the expression of a gene of interest.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731300">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member bitleft">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Andrea.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">K731400 - IPTG inducible Cysteine desulfhydrase (CysDes)</a></h2>
+
-
<p>This part is composed of K731300 and K731600. It is an IPTG inducible device regulating the expression of a Cysteine Desulfhydrase from Treponema Denticola (CysDes). This enzyme is an aminotransferase that converts cysteine into pyruvate, ammonia and hydrogen sulfide. The part has been successfully operated and characterized in psB1C3 and psB4K5.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Andrea.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731480">K731480 - IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)</a></h2>
+
-
<p>A sfGFP tagged CysDes inducible by IPTG.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731480">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member bitleft">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Gugu.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731500">K731500 - Inducibile tac promoter and lac operon</a></h2>
+
-
<p>This is E.coli lactose-inducibile promoter.
+
-
lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription. Low, constitutive levels of transcription can be seen anyhow.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731500">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Gugu.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731520">K731520 - Lactose-inducible sfGFP</a></h2>
+
-
<p>This is a construct carrying the sfGFP gene with low-efficiency RBS, under lac operator control.
+
-
lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.Low, constitutive levels of transcription can be seen anyhow.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731520">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member bitleft">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Andrea.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731600">K731600 - Cysteine Desulfhydrase (CysDes)</a></h2>
+
-
<p>This part encode for a cysteine desulfhydrase from Treponema denticola, which is known to be a strong enzyme that produces H2S, ammonia and pyruvate starting from cysteine. It is often indicated also as Hly.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731600">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Anna.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">K731700 - Low copy vector for terminators analysis with T7 RNApolymerase by fluorimetric assay</a></h2>
+
-
<p>This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus), separated by Prefix-Suffix linker, allow standardization of terminator's presence effect. The vector is a modified pET21b; it contains Ampicillin resistance gene and is inducible by IPTG (T7promoter-lacO).
+
-
The combined use of BBa_K731710 and BBa_K731700 allow also to analyse any potential difference in terminators' activity due to different polymerases.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member bitleft">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Anna.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">K731701 - BBa_K731700 with T7terminator (BBa_K731721)</a></h2>
+
-
<p>T7 terminator T7 polymerase testing construct.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Anna.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731702">K731702 - BBa_K731700 with BBa_K731722 terminator (BBa_B0010)</a></h2>
+
-
<p>Coli terminator T7 polymerase testing construct.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731702">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member bitleft">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Anna.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731710">K731710 - Low copy vector for terminators analysis with Coli RNApolymerase by fluorimetric assay</a></h2>
+
-
<p style="font-size: 11px;">This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus), separated by Prefix-Suffix linker, allow standardization of terminator's presence effect. The vector was obained by mutagenesis of BBa_K731700, replacing T7 promoter with pTAC. It contains Ampicillin resistance gene and is inducible by IPTG (pTAC-lacO).
+
-
The combined use of BBa_K731710 and BBa_K731700 allow also to analyse any potential difference in terminators' activity due to different polymerases.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731710">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Anna.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731">K731711 - BBa_K731710 with T7terminator (BBa_K731721)</a></h2>
+
-
<p>T7 terminator coli polymerase testing construct.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member bitleft">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Anna.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731712">K731712 - BBa_K731710 with BBa_K731722 terminator (BBa_B0010)</a></h2>
+
-
<p>coli terminator coli polymerase testing construct 2.</p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731712">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Anna.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731721">K731721 - T7 terminator</a></h2>
+
-
<p>Gag ipsum dolar sit amet venenatis charlie sheen lose i'm watching u. Female weekend finals close enough derp top note so close architect yao. Face oboma unlock ipsum t-rex keyboard just simpson gag elephant bacon cuteness overload. </p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731721">Registry Page</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="member bitleft">
+
-
<img src="http://www.jasonfweb.com/igem2/img/team/Anna.jpg" alt="" />
+
-
<div class=partDescription>
+
-
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">K731722 - B0010 Coli terminator</a></h2>
+
-
<p>Gag ipsum dolar sit amet venenatis charlie sheen lose i'm watching u. Female weekend finals close enough derp top note so close architect yao. Face oboma unlock ipsum t-rex keyboard just simpson gag elephant bacon cuteness overload. </p>
+
-
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">Registry Page</a>
+
-
</div>
+
-
</div>-->
+
 +
<div class="Part green">
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731520">K731520 IPTG inducible GFPmut3b</a></h2>
 +
<p>A device for the characterization of our new <a href="http://partsregistry.org/Part:BBa_K731500">BBa_K731500</a> followed by a strong RBS and GFPmut3b. Also available in pSB4K5.</p>
 +
</div>
</div> <!--end content-->
</div> <!--end content-->
Line 418: Line 354:
<div id="sponsor">
<div id="sponsor">
<ul>
<ul>
-
<li><a href="http://www.fondazionecassaruraleditrento.it"><img src="http://www.jasonfweb.com/igem2/img/sponsor/CRT.jpg" alt="" /></a></li>
+
<li><a href="http://www.fondazionecassaruraleditrento.it"><img src="http://www.science.unitn.it/~igem/img/sponsor/CRT.jpg" alt="" /></a></li>
-
<li><a href="http://www.unitn.it/en/cibio"><img src="http://www.jasonfweb.com/igem2/img/sponsor/CIBIO.png" alt="" style="height:40px"/></a></li>
+
<li><a href="http://www.unitn.it/en/cibio"><img src="http://www.science.unitn.it/~igem/img/sponsor/CIBIO.png" alt="" style="height:50px"/></a></li>
-
<li><a href="http://www.unitn.it/en"><img src="http://www.jasonfweb.com/igem2/img/sponsor/UniTN.JPG" alt="" style="height:40px; position: relative; top: 3px;" /></a></li>
+
<!--<li><a href="http://www.unitn.it/en"><img src="http://www.science.unitn.it/~igem/img/sponsor/UniTN.JPG" alt="" style="height:40px; position: relative; top: 3px;" /></a></li>-->
</ul>
</ul>
 +
<img class="unitn" src="http://www.science.unitn.it/~igem/img/sponsor/UniTN.JPG" alt="" />
</div>
</div>
</div>
</div>
Line 456: Line 393:
-
<p>Design by Jason Fontana. Powered by <a href="http://www.mediawiki.org/wiki/MediaWiki">MediaWiki</a>. This website is under the <a href="http://creativecommons.org/licenses/by/3.0/">CC Attribution licence</a>. | <span class="toggle">Toggle controls</span></p>
+
<p>Design by Jason Fontana. Powered by <a href="http://www.mediawiki.org/wiki/MediaWiki">MediaWiki</a>. This website is under the <a href="http://creativecommons.org/licenses/by/3.0/">CC Attribution licence</a>.<!-- | <span class="toggle">Toggle controls</span>--></p>
</div>
</div>
</div>
</div>
-
<script>
+
 
-
(function() {
+
<!--<script>
-
$.ajaxSetup({cache:true});
+
-
$.getScript('http://static.tumblr.com/g8b5dgd/Jzdm1nbnz/jquery.tweet-min.js',function(){
+
-
$(".tweets").tweet({
+
-
username: "jasonfweb",
+
-
join_text: "auto",
+
-
count: 1,
+
-
auto_join_text_default: "we said,",
+
-
auto_join_text_ed: "we",
+
-
auto_join_text_ing: "we were",
+
-
auto_join_text_reply: "we replied to",
+
-
auto_join_text_url: "we were checking out",
+
-
loading_text: "Getting latest tweet..."
+
-
}).bind('loaded', function () {
+
-
$('.twitter-feed').fadeTo('fast', 1);
+
-
});
+
-
}); })();
+
-
</script>
+
-
<script>
+
(function() {
(function() {
var $mw = $('div#mw');
var $mw = $('div#mw');
Line 488: Line 407:
});
});
})();
})();
-
</script>
+
</script> -->
<script type="text/javascript">
<script type="text/javascript">

Latest revision as of 17:34, 25 September 2012

Our Parts

It appears you have JavaScript disabled. Our Wiki needs it for you to enjoy the full experience. Please enable Javascript and refresh the page.

Our Parts

Here you can find our Best Parts and all the Parts we built. Make sure to check out our Team Parts Page on the Registry website!

BEST PARTS

K731030 - Inducible araC-pBAD promoter regulating M256I CysE

A serine acetyltransferase (CysE) controlled by an arabinose inducible system.
This is a key part in our black crust project: a device used to reduce sulfate and produce cysteine.

K731400 - IPTG inducible Cysteine desulfhydrase (CysDes)

Our favorite BioBrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key part for the Crust Away project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid.

K731722 - rrnB T1 terminator

Our new entry in the terminator collection of the Registry: a functioning version of BBa_B0010! This terminator was characterized with both T7 and E. coli RNA polymerases.

All Our Parts

Please note that many parts have been also subcloned into low copy vectors. Although these parts have note been submitted to the registry, they are available upon request. Contact us at igemtrento [at] gmail [dot] com

The Black Crust Enzymes

K731000 CysE (Serine Acetyltransferase)

A Part encoding CysE, our key enzyme in the reduction of sulfate.

K731010 M256I CysE (Serine Acetyltransferase)

This is a mutated CysE. Introduction of a Methionine at position 256, makes our key player even "stronger" and enhances sulfate uptake.

K731020 Inducible araC-pBAD promoter regulating WT CysE

A device to produce CysE upon induction with arabinose. Also available in pSB3C5.

K731030 Inducible araC-pBAD promoter regulating M256I CysE

A device for the production of M256I CysE controlled by an arabinose inducible cassette. This Part was used for the bioremoval of the black crust from different stones samples. Also available in pSB3C5.

K731040 Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE

This is our favorite Part BBa_K731030 followed by a sfGFP. Created by Gibson assembly.

K731400 IPTG inducible Cysteine desulfhydrase (CysDes)

Our favorite Bi Bbrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key Part for the Crust Away project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid. Also available in pSB4K5.

K731480 IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)

This is a sfGFP tagged version of our best BioBrick BBa_K731400. A superfluo device created by Gibson assembly. Also available in pSB4K5.

Terminators

K731721 T7 terminator

Our new entry in the terminator collections of the Registry: a T7 terminator! This terminator was characterized in vivo and in vitro with both T7 and E. coli polymerases.

K731722 rrnB T1 terminator E. coli terminator

A good working alternative for the E.coli terminator BBa_B0010.

K731700 Platform for terminators analysis under the control of T7 promoter

Our measurement device for terminators characterization. A T7 promoter controlling the expression of A206K Venus and mCherry for the ratiometric analysis of terminator efficiency.

K731710 Platform for terminators analysis under the control of TAC promoter

Our great platform BBa_K731722, where T7 promoter was substituted with Ptac.

K731701 BBa_K731700 with T7terminator (BBa_K731721)

A measurement device used to characterize T7 terminator under the control of T7 promoter.

K731702 BBa_K731700 with BBa_K731722 rrnB T1 terminator (BBa_B0010)

A measurement device used to characterize B0010 terminator under the control of T7 promoter.

K731711 BBa_K731710 with T7 terminator (BBa_K731721)

A measurement device used to characterize the T7 terminator (BBa_K731721) under the control of tac promoter.

K731712 BBa_K731710 with BBa_K731722 rrnB T1 terminator (BBa_B0010)

A measurement device used to characterize the E.coli terminator (BBa_K731722) under the control of tac promoter.

Promoters

K731201 Inducible araC-pBAD promoter

A new version of the araC-pBad promoter that was amplified from the E.coli genome. An excellent alternative to BBa_I0050.

K731300 LacI + LacIq promoter (reverse)

A ready to go IPTG inducible cassette that is composed of LacI controlled by the lacIq promoter, both in the reverse direction.

K731500 [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward

Our Part BBa_K731300 followed by a tac promoter and a lac operator. The perfect BioBrick for those interested in expressing their gene under the control of IPTG. It will save you a lot of cloning steps! Also available in pSB4K5.

K731250 Inducible araC-pBAD promoter with GFPmut3b, strong RBS

A device for the characterization of our new entry araC-pBad (BBa_K731201) followed by a strong RBS and a GFPmut3b. Also available in pSB3C5.

K731255 Inducible araC-pBAD promoter with GFPmut3b, weak RBS

A device for the characterization of our new entry araC-pBad (BBa_K731201) followed by a weak RBS and GFPmut3b.

K731520 IPTG inducible GFPmut3b

A device for the characterization of our new BBa_K731500 followed by a strong RBS and GFPmut3b. Also available in pSB4K5.

Retrieved from "http://2012.igem.org/Team:UNITN-Trento/Parts"