Team:KIT-Kyoto/Notebook-week7

September 18th

By red eye screening, we identified the strain 7 and strain 10 to be the successfully transformed lines. Photographs for the transformed red (yellow) eye fly (strain 10) were taken.

September 19th

By red eye screening, we identified the strain 13 and strain 14 to be the successfully transformed lines. Photographs for the transformed red (orange) eye fly (strain 13) were taken.



Drosophila S2 cells (2.5 X 10⁵ cells per well) were plated on the 24 well plate and cultured in the Schneider’s Drosophila medium containing 10% fetal bovine serum at 25 ℃.

September 20th

By red eye screening, we identified the strain 23 and strain 29 to be the successfully transformed lines.

September 21st

Red eye screening was continued.

September 22nd

Red eye screening was continued.

September 23rd

By red eye screening, we identified the strain 56 and strain 65 to be the successfully transformed lines. These transformed flies are kept at 25℃ and later will be further inspected for chromosome linkage of the transgene.