Team:Osaka

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  <b>Our team project：<font color="red">BIO-DOSIMETER</font></b>                     
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  <b>Our team project：<font color="blue">BIO-DOSIMETER</font></b>                     
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In the modern world, radioactivity is a phenomena well understood by science, and harnessed by engineering for nuclear power generation, radiology in medicine, radioisotopic labeling in biological studies and numerous other applications. However, it is an undeniable fact that the ionizing radiation resulting from radioactive decay may cause critical effects on biological creatures, from the smallest bacteria to plants and animals including humans.
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  On March 11, 2011, the Great East Japan Earthquake struck off the coast of Eastern Japan and triggered a series of equipment failures, nuclear meltdowns, and releases of radioactive materials at the Fukushima 1 Nuclear Power Plant, leading to a nationwide nuclear crisis. <img src="https://static.igem.org/mediawiki/2011/b/b9/Nuclear_1.jpg.jpg" height="250" align="right" alt="the crisis of Japanese nuclea plants">While ‘Grays’, ‘Sieverts’, ‘exposure’, ‘equivalent dosage’ and other related terms and units became referred daily in the media, much of the general populace remained ignorant of their meanings and significance. The need for low-cost, portable and easy-to-use dosimeters was apparent as measurements of radiation exposure could only be conducted at dedicated installations spaced far apart and the numbers reported only infrequently.
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  It is still sharp in our memory that, on March 11, 2011, the Great East Japan Earthquake struck off the coast of Eastern Japan and triggered a series of equipment failures, nuclear meltdowns, and releases of radioactive materials at the Fukushima 1 Nuclear Power Plant, leading to the nationwide nuclear crisis.  
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While ‘Grays’, ‘Sieverts’, ‘exposure’, ‘equivalent dosage’ and other related terms and units became referred daily in the media, much of the general populace remained ignorant of their meanings and significance. The need for low-cost, portable and easy-to-use dosimeters was apparent as measurements of radiation exposure could only be conducted at dedicated installations spaced far apart and the numbers reported only infrequently.
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  Hence, this year, iGEM Osaka decided to tackle the building of a biological dosimeter. We attempted to modularly transfer radiation-response and DNA repair genes from the extremophilic bacterium <i>Deinococcus radiodurans</i> to the well-characterized, easily cultivable chassis of <i>Escherichia coli</i>. From there our efforts branched out into (1) evaluating radioresistance conferred by the heterologous genes, and (2) connecting the radiation/DNA damage response system to visible outputs such as color pigment production.
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  Moved by that accident in iGEM 2011, we have built a synthetic biological dosimeter to detect the radiation. In this year we further develop that "Bio-dosimeter". Our "Bio-dosimeter" consists of two points: <b>damage tolerance</b> and <b>radiation detection</b>. To introduce the tolerance to <i>E. coli</i>, we are trying to put in some radiation resistance genes from <i>Deinococcus radiodurans</i>. For the detection of the radiation, we are trying to connect the native DNA damage response system of <i>E. coli</i> to production of pigment lycopene as a reporter.
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Last year, we cloned selected genes from radiodurans genomic DNA and BioBricked them. We then conferred additional UV-induced DNA damage tolerance by transforming certain genes into <i>E.coli</i>. Now, we are attempting to assess its tolerance to various types of DNA damage and to evaluate DNA damage detection more clearly.
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　■　Sponsors                         
　■　Sponsors                         
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<a href="https://2011.igem.org/Team:Osaka/we_love_JAPAN">
 
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<p><a href="http://www.osaka-u.ac.jp/en/index.html">
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<p><a href="http://www.frc.eng.osaka-u.ac.jp/">
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<br>Contact us :  xiaoshunmeng@gmail.com
<br>Contact us :  xiaoshunmeng@gmail.com
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Latest revision as of 05:14, 25 September 2012



Our team project：BIO-DOSIMETER


It is still sharp in our memory that, on March 11, 2011, the Great East Japan Earthquake struck off the coast of Eastern Japan and triggered a series of equipment failures, nuclear meltdowns, and releases of radioactive materials at the Fukushima 1 Nuclear Power Plant, leading to the nationwide nuclear crisis. While ‘Grays’, ‘Sieverts’, ‘exposure’, ‘equivalent dosage’ and other related terms and units became referred daily in the media, much of the general populace remained ignorant of their meanings and significance. The need for low-cost, portable and easy-to-use dosimeters was apparent as measurements of radiation exposure could only be conducted at dedicated installations spaced far apart and the numbers reported only infrequently.

Moved by that accident in iGEM 2011, we have built a synthetic biological dosimeter to detect the radiation. In this year we further develop that "Bio-dosimeter". Our "Bio-dosimeter" consists of two points: damage tolerance and radiation detection. To introduce the tolerance to E. coli, we are trying to put in some radiation resistance genes from Deinococcus radiodurans. For the detection of the radiation, we are trying to connect the native DNA damage response system of E. coli to production of pigment lycopene as a reporter.

Last year, we cloned selected genes from radiodurans genomic DNA and BioBricked them. We then conferred additional UV-induced DNA damage tolerance by transforming certain genes into E.coli. Now, we are attempting to assess its tolerance to various types of DNA damage and to evaluate DNA damage detection more clearly.

　■　Sponsors



Contact us : xiaoshunmeng@gmail.com

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