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Team:TU Darmstadt/Protocols/Glycerine stock
From 2012.igem.org
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In order to have a permanent culture of cells glycerine stocks can be made. | In order to have a permanent culture of cells glycerine stocks can be made. | ||
=== Protocol === | === Protocol === | ||
| - | * Add 200 µl of sterilized glycerine to 800 µl cell culture and mix well | + | * Add 200 µl of sterilized glycerine or DMSO to 800 µl cell culture and mix well |
* Freeze the stock at -20 °C | * Freeze the stock at -20 °C | ||
Revision as of 15:32, 24 September 2012
Glycerine stock
In order to have a permanent culture of cells glycerine stocks can be made.
Protocol
- Add 200 µl of sterilized glycerine or DMSO to 800 µl cell culture and mix well
- Freeze the stock at -20 °C
