Team:Wageningen UR/Results

From 2012.igem.org

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*[[Transformation of E.coli]]
 
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*[[Sequencing of PLRV CP's]]
 
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*[[Alignment experiments]]
 
== General ==
== General ==

Revision as of 15:11, 24 September 2012

Contents

Results

The Constructor

More efficient cloning, publication, importance of computational work in SynBio

iGEM 2011 team iGEM project title # of TU's in the designed circuit # of used Biobrick Parts By team Recommended by the Constructor Processing time (min)
KAIST-Korea1 E. casso 2 11 7 < 1 min
UANL_Mty-Mexico2 S.C.I.E.N.C.E. : Simple Code Interpretation Enabling Circuit in E. coli 7 26 25 674 min
XMU-China3 i-ccdB: intelligent Control of Cell Density in Bacteria 3 6 6 < 1 min
NTNU-Trondheim4 Red Fluorescent Stress Sensor 2 5 4 < 1 min
Wageningen UR5 The Synchroscillator: a Synchronized Oscillatory System 4 7 6 < 2 min

1 https://2011.igem.org/Team:KAIST-Korea

2 https://2011.igem.org/Team:UANL_Mty-Mexico

3 https://2011.igem.org/Team:XMU-China

4 https://2011.igem.org/Team:NTNU_Trondheim

5 https://2011.igem.org/Team:Wageningen_UR


Our publication of The Constructor at the Journal of Biological Engineering [Highly Accessed].


The poster of The Constructor, presented at the German Synthetic Biology Conference in May 2012 in Munich.

Plug and Apply System

GFP-coil, coils, the idea

Outside Modifications

  • Designed the primers for amplification of the needed constructs
  • Delivered the proof of principle that these primers work [link to framshift, Hugo]
  • Provided the templates for modifications and placed them in the partsregistry
  • Isolated the PLRV from nature which, in theory, should be easy to modify on the outside

Inside Modifications

Negative CCMV, Frameshift

Virus Like particles

Detection Isolation from nature

General

  • We organised a mini Symposium for all dutch teams in the begining of the competition to create a national network and eventualy a national effort
  • We visited secondary schools to teach them about synthetic biology and let the make leafelets about synthetic biology.
  • We went to present our project and The Constructor at the Centre of Advanced Studies Synthetic Biology conference in Munich, where we learned a lot and get familiar with the German iGEM teams
  • We communicated to a graphical designer to obtain an kinetic graphical movie of our VLP formation, that is included in our introduction page movie.
  • We involved a communication study in our discovery festival human practises, so that we do not only inform the public, but also will have results on how scientist communicate with the public.
  • We have taken a number of inverviews with stakeholders to have an opinion of expert on our project, regulations around our project and about the idea of an open source environment