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Team:UNITN-Trento/Attributions
From 2012.igem.org
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<p>The idea of Crustaway has been always in the back of our minds since Daniele proposed it in our first meeting in March. <br /> | <p>The idea of Crustaway has been always in the back of our minds since Daniele proposed it in our first meeting in March. <br /> | ||
However, the idea started to take off when Jason found an interesting paper by J. Keasling where they used two genes to reduce sulfate with the ultimate goal of precipitating metals. Since then everybody worked on the planning of the project. <br /> | However, the idea started to take off when Jason found an interesting paper by J. Keasling where they used two genes to reduce sulfate with the ultimate goal of precipitating metals. Since then everybody worked on the planning of the project. <br /> | ||
| - | Anna and Giacomo with some help from Sheref designed the Terminator 5 project.</p> | + | Anna and Giacomo with some help from Sheref (one of our supervisors) designed the Terminator 5 project.</p> |
<h3>CONSTRUCT DESIGN</h3> | <h3>CONSTRUCT DESIGN</h3> | ||
<h4>Crust Away</h4> | <h4>Crust Away</h4> | ||
| - | <p>Most of the constructs were planned by Giacomo and Daniele, with some help from Cristina.<br /> | + | <p>Most of the constructs were planned by Giacomo and Daniele, with some help from Cristina (our supervisor).<br /> |
The constructs for the GFP fusion were designed by Jason, Andrea and Francesco.</p> | The constructs for the GFP fusion were designed by Jason, Andrea and Francesco.</p> | ||
<h4>Terminator 5</h4> | <h4>Terminator 5</h4> | ||
| - | <p>We started to build our platform from a plasmid of the Mansy lab (RL024), made by one of our advisors Roberta Lentini. Starting from this construct we mutated three illegal sites and we added a prefix and a suffix between the two fluorescent proteins.<br /> | + | <p>We started to build our platform from a plasmid of the Mansy lab (RL024), made by one of our advisors Roberta Lentini (one of our advisors). Starting from this construct we mutated three illegal sites and we added a prefix and a suffix between the two fluorescent proteins.<br /> |
All the constructs for this project were designed by Giacomo and Anna, with initial help of Cristina.</p> | All the constructs for this project were designed by Giacomo and Anna, with initial help of Cristina.</p> | ||
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Gas chromatography measurements were done by Damiano Avi.</p> | Gas chromatography measurements were done by Damiano Avi.</p> | ||
<h4>CysE Parts:</h4> | <h4>CysE Parts:</h4> | ||
| - | <p>Jason has cloned and characterized Parts: K731000, K731010, K731020, K731030, K731025 (i.e. | + | <p>Jason has cloned and characterized Parts: K731000, K731010, K731020, K731030, K731025 (i.e. K731020 in pSB3C5), K731035 (i.e. K731030 in pSB3C5), K731040, K731045 (i.e. K731040 in pSB3C5).</p> |
<h4>Promoters Parts:</h4> | <h4>Promoters Parts:</h4> | ||
| - | <p>Daniele worked on the following Parts: K731200, K731201, K731250, K731255 , K731260 (i.e. | + | <p>Daniele worked on the following Parts: K731200, K731201, K731250, K731255 , K731260 (i.e. K731250 in pSB3C5).<br /> |
Daniele also helped Jason with the characterization of K731040.<br /> | Daniele also helped Jason with the characterization of K731040.<br /> | ||
Francesco built and characterized Parts: K731500, K731510, K731520, K731530 and gave some help in characterizing K731480.</p> | Francesco built and characterized Parts: K731500, K731510, K731520, K731530 and gave some help in characterizing K731480.</p> | ||
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Everybody had fun together cleaning the statues!</p> | Everybody had fun together cleaning the statues!</p> | ||
| - | <p>Scanning Electron Microscopy Images were taken at the facility of SEM in the department of Engineering.</p> | + | <p>Scanning Electron Microscopy Images were taken at the facility of SEM in the department of Engineering by the staff of the facility under the supervision of Prof. Flavio DeFlorian.</p> |
<h3>WEBSITE</h3> | <h3>WEBSITE</h3> | ||
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<h3>POSTER, PRESENTATION & LOGO</h3> | <h3>POSTER, PRESENTATION & LOGO</h3> | ||
| - | <p>Our poster was designed by Andrea with some help from Laura.<br /> | + | <p>Our poster was designed by Andrea with some help from Laura (our other advisor).<br /> |
The logo was designed by Daniele.<br /> | The logo was designed by Daniele.<br /> | ||
Everybody worked on the presentation.</p> | Everybody worked on the presentation.</p> | ||
Revision as of 08:36, 24 September 2012
Attributions
IDEA
The idea of Crustaway has been always in the back of our minds since Daniele proposed it in our first meeting in March.
However, the idea started to take off when Jason found an interesting paper by J. Keasling where they used two genes to reduce sulfate with the ultimate goal of precipitating metals. Since then everybody worked on the planning of the project.
Anna and Giacomo with some help from Sheref (one of our supervisors) designed the Terminator 5 project.
CONSTRUCT DESIGN
Crust Away
Most of the constructs were planned by Giacomo and Daniele, with some help from Cristina (our supervisor).
The constructs for the GFP fusion were designed by Jason, Andrea and Francesco.
Terminator 5
We started to build our platform from a plasmid of the Mansy lab (RL024), made by one of our advisors Roberta Lentini (one of our advisors). Starting from this construct we mutated three illegal sites and we added a prefix and a suffix between the two fluorescent proteins.
All the constructs for this project were designed by Giacomo and Anna, with initial help of Cristina.
CLONING & CHARACTERIZATION
CysDes Parts:
Andrea built and characterized Parts: K731300, K731400, K731450 (i.e. K731400 in pSB4K5), K731480.
The initial gene encoding CysDes was synthetized by Genescript and then subcloned into our Parts.
Gas chromatography measurements were done by Damiano Avi.
CysE Parts:
Jason has cloned and characterized Parts: K731000, K731010, K731020, K731030, K731025 (i.e. K731020 in pSB3C5), K731035 (i.e. K731030 in pSB3C5), K731040, K731045 (i.e. K731040 in pSB3C5).
Promoters Parts:
Daniele worked on the following Parts: K731200, K731201, K731250, K731255 , K731260 (i.e. K731250 in pSB3C5).
Daniele also helped Jason with the characterization of K731040.
Francesco built and characterized Parts: K731500, K731510, K731520, K731530 and gave some help in characterizing K731480.
Terminators Parts:
Anna and Giacomo built and characterized together all the Parts for the terminators project: K731700, K731701, K731710, K731711, K731721, K731722.
Anna and Giacomo developed a new method to use the platform. Sheref contributed with both humor and important suggestions.
APPLICATIONS
The black crust bioremediation pathway was put together by Daniele, Francesco e Jason.
The Crustanator was an idea of Cristina and was built by Daniele and Francesco with some technical suggestions of Damiano.
Everybody had fun together cleaning the statues!
Scanning Electron Microscopy Images were taken at the facility of SEM in the department of Engineering by the staff of the facility under the supervision of Prof. Flavio DeFlorian.
WEBSITE
Our Wiki was designed by Jason, who also put all the content together. Everybody worked on the content of the Wiki.
ART & SCIENCE
Although, we all took part to the interviews, this aspect of the project was mostly cured by Andrea and Anna.
EVENTS
Anna organized with Roberta the “3 open days” at the Museum of Science for high school teachers.
Francesco and Roberta organized The Researchers Night.
Everybody participated to the events.
SAFETY
Francesco and Cristina wrote the SRB safety handbook. Marzia Filippi from the Safety office of the University of Trento revised the text. UniTN communication office helped with the graphic of the pamphlet.
POSTER, PRESENTATION & LOGO
Our poster was designed by Andrea with some help from Laura (our other advisor).
The logo was designed by Daniele.
Everybody worked on the presentation.
TRAINING and ICECREAMS…
…were brought in by Cristina.
