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Team:Bordeaux/Sixth
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<li><a href="https://2012.igem.org/Team:Bordeaux/Fifth">Fifth Week</a></li> | <li><a href="https://2012.igem.org/Team:Bordeaux/Fifth">Fifth Week</a></li> | ||
<li><a href="https://2012.igem.org/Team:Bordeaux/Sixth" class="current">Sixth Week</a></li> | <li><a href="https://2012.igem.org/Team:Bordeaux/Sixth" class="current">Sixth Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Seventh">Seventh Week</a></li> | ||
</ul> | </ul> | ||
Revision as of 06:59, 24 September 2012
Day 24 : 06-08-2012
As the transformation didn't gave any colonies on the plate We decided to try again , and control on agarose gel as the gel seemed promising we transformed 2 μL of plasmid DNA We also tester the cells competence by transforming 1μg of pBlueScript and pSB1A3.
Day 25 : 07-08-2012
No colonies on the plates again ... even on the two tests we clearly have a competence issue. So we need to make a new stock today we tested the biobricks from day 19 on agarose gels
Day 26 : 08-08-2012
Today we did a miniprep with the IAIB precultures from yesterday We got poor results from 14ng/μL to 36,6ng/μL DNA on the tubes We runned simple digested samples on an agarose gel but we couldn't discriminate the difference between the vector only and the IAIB+vector samples due to a too small deferences We'd have to run a more concentrated gel. We then tried the assembly of IAIB ICIE IIAIIB IIC(1)5B We digested the bricks and tested them on agarose gel.
Day 27 : 09-08-2012
Nothing on the plates ... Except for the DH5α pBluescript test
