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Team:Wageningen UR/Results
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== General == | == General == | ||
| - | We organised a [[Team:Wageningen_UR/miniSymposium|mini Symposium]] for all dutch teams in the begining of the competition to create a national network and eventualy | + | We organised a [[Team:Wageningen_UR/miniSymposium|mini Symposium]] for all dutch teams in the begining of the competition to create a national network and eventualy a [[Team:Wageningen_UR/DiscoveryFestival|national effort]] |
Human Practices | Human Practices | ||
We went to present our project and [[Team:Wageningen_UR/TheConstructor|The Constructor]] at the Centre of Advanced Studies Synthetic Biology conference in Munich, where we learned a lot and get familiar with the German iGEM teams | We went to present our project and [[Team:Wageningen_UR/TheConstructor|The Constructor]] at the Centre of Advanced Studies Synthetic Biology conference in Munich, where we learned a lot and get familiar with the German iGEM teams | ||
Revision as of 15:50, 23 September 2012
Contents |
Results
The Constructor
More efficient cloning, publication, importance of computational work in SynBio
| iGEM 2011 team | iGEM project title | # of TU's in the designed circuit | # of used Biobrick Parts By team | Recommended by the Constructor | Processing time (min) |
|---|---|---|---|---|---|
| KAIST-Korea1 | E. casso | 2 | 11 | 7 | < 1 min |
| UANL_Mty-Mexico2 | S.C.I.E.N.C.E. : Simple Code Interpretation Enabling Circuit in E. coli | 7 | 26 | 25 | 674 min |
| XMU-China3 | i-ccdB: intelligent Control of Cell Density in Bacteria | 3 | 6 | 6 | < 1 min |
| NTNU-Trondheim4 | Red Fluorescent Stress Sensor | 2 | 5 | 4 | < 1 min |
| Wageningen UR5 | The Synchroscillator: a Synchronized Oscillatory System | 4 | 7 | 6 | < 2 min |
1 https://2011.igem.org/Team:KAIST-Korea
2 https://2011.igem.org/Team:UANL_Mty-Mexico
3 https://2011.igem.org/Team:XMU-China
4 https://2011.igem.org/Team:NTNU_Trondheim
5 https://2011.igem.org/Team:Wageningen_UR
Our publication of The Constructor at the Journal of Biological Engineering [Highly Accessed].
Plug and Apply System
GFP-coil, coils, the idea
Outside Modifications
- Designed the primers for amplification of the needed constructs
- Delivered the proof of principle that these primers work [link to framshift, Hugo]
- Provided the templates for modifications and placed them in the partsregistry
- Isolated the PLRV from nature which, in theory, should be easy to modify on the outside
Inside Modifications
Negative CCMV, Frameshift
Virus Like particles
Detection Isolation from nature
- RNA isolation
- RT-PCR
- Addition of prefix and suffix
- Transformation of E.coli
- Isolation of plasmids
- Sequencing of PLRV CP's
- Alignment experiments
General
We organised a mini Symposium for all dutch teams in the begining of the competition to create a national network and eventualy a national effort
Human Practices
We went to present our project and The Constructor at the Centre of Advanced Studies Synthetic Biology conference in Munich, where we learned a lot and get familiar with the German iGEM teams
