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Team:Wageningen UR/Results
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== General == | == General == | ||
| - | We organised a [[Team:Wageningen_UR/miniSymposium|mini Symposium]]for all dutch teams in the begining of the competition to create a national network and eventualy an[[Team:Wageningen_UR/ | + | We organised a [[Team:Wageningen_UR/miniSymposium|mini Symposium]]for all dutch teams in the begining of the competition to create a national network and eventualy an[[Team:Wageningen_UR/DiscoveryFestival|national effort]] |
Human Practices | Human Practices | ||
We went to present our project and [[Team:Wageningen_UR/TheConstructor|The Constructor]] at the Centre of Advanced Studies Synthetic Biology conference in Munich, where we learned a lot and get familiar with the German iGEM teams | We went to present our project and [[Team:Wageningen_UR/TheConstructor|The Constructor]] at the Centre of Advanced Studies Synthetic Biology conference in Munich, where we learned a lot and get familiar with the German iGEM teams | ||
Revision as of 15:49, 23 September 2012
Contents |
Results
The Constructor
More efficient cloning, publication, importance of computational work in SynBio
| iGEM 2011 team | iGEM project title | # of TU's in the designed circuit | # of used Biobrick Parts By team | Recommended by the Constructor | Processing time (min) |
|---|---|---|---|---|---|
| KAIST-Korea1 | E. casso | 2 | 11 | 7 | < 1 min |
| UANL_Mty-Mexico2 | S.C.I.E.N.C.E. : Simple Code Interpretation Enabling Circuit in E. coli | 7 | 26 | 25 | 674 min |
| XMU-China3 | i-ccdB: intelligent Control of Cell Density in Bacteria | 3 | 6 | 6 | < 1 min |
| NTNU-Trondheim4 | Red Fluorescent Stress Sensor | 2 | 5 | 4 | < 1 min |
| Wageningen UR5 | The Synchroscillator: a Synchronized Oscillatory System | 4 | 7 | 6 | < 2 min |
1 https://2011.igem.org/Team:KAIST-Korea
2 https://2011.igem.org/Team:UANL_Mty-Mexico
3 https://2011.igem.org/Team:XMU-China
4 https://2011.igem.org/Team:NTNU_Trondheim
5 https://2011.igem.org/Team:Wageningen_UR
Our publication of The Constructor at the Journal of Biological Engineering [Highly Accessed].
Plug and Apply System
GFP-coil, coils, the idea
Outside Modifications
- Designed the primers for amplification of the needed constructs
- Delivered the proof of principle that these primers work [link to framshift, Hugo]
- Provided the templates for modifications and placed them in the partsregistry
- Isolated the PLRV from nature which, in theory, should be easy to modify on the outside
Inside Modifications
Negative CCMV, Frameshift
Virus Like particles
Detection Isolation from nature
- RNA isolation
- RT-PCR
- Addition of prefix and suffix
- Transformation of E.coli
- Isolation of plasmids
- Sequencing of PLRV CP's
- Alignment experiments
General
We organised a mini Symposiumfor all dutch teams in the begining of the competition to create a national network and eventualy annational effort
Human Practices
We went to present our project and The Constructor at the Centre of Advanced Studies Synthetic Biology conference in Munich, where we learned a lot and get familiar with the German iGEM teams
