Team:Amsterdam/project/growthrates

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Revision as of 09:21, 23 September 2012

Growth curve experiments

The division rate of the cells is of vital importance to the duration in which the registered signal is stored in the cell. The slower the growth rate of the Cellular Logbook, the slower the dilution of the methylated plasmids and hence the longer the stored signal is retained. The impact on the growth rates of the various constructs we have gives an indication of their transcriptional burden.

This is why We´ve performed growth experiments for two constructs: pSB1AT3-pLac-MTase and pSB1AT3-pBAD-MTase. At this time we didn't have our more complete construct including the Zinc Finger prepared yet. Both experiments were performed in cell strains <math>\text{DH5}\alpha</math>.

Using the Mathematica function NonLinearModelFit functions of the form <math>a + b e^{c x}</math> were fitted to the exponential phases of the growth curves.

Strain	Function
pLac + IPTG	<math>-0.0501829+0.0743786 e^{0.0089986 x}</math>
pLac - IPTG	<math>-0.0823022+0.0938526 e^{0.00812998 x} </math>
pBAD + Arabinose	<math>-0.0101962+0.0614008 e^{0.00889096 x} </math>
pBAD - Arabinose	<math>-0.0233633+0.0489266 e^{0.00830895 x} </math>

@@ Line 16: / Line 16: @@
 Both experiments were performed in cell strains <math>\text{DH5}\alpha</math>.
-[[File:rateestimation.png]]
+[[File:rateestimation.png|Growth rates of two different constructs (pLac, pBAD) with either the corresponding signal (lactose, arabninose) present or not present]]
-<br>
-Growth rates of two different constructs (pLac, pBAD) with either the corresponding signal (lactose, arabninose) present or not present.
 Using the Mathematica function <code>NonLinearModelFit</code> functions of the form <math>a + b e^{c x}</math> were fitted to the exponential phases of the growth curves.