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| | {{:Team:EPF-Lausanne/Template/LabPresence|Sowmya, Pascal, June, Diego}} | | {{:Team:EPF-Lausanne/Template/LabPresence|Sowmya, Pascal, June, Diego}} |
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| - | == 05.07.12 transfection == | + | == Checking on cells from the 05.07.12 transfection == |
| | Cell counting. | | Cell counting. |
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| | * Well plate taken from Elisa room (CHB1374). | | * Well plate taken from Elisa room (CHB1374). |
| | * Sampled 200 µl from each of the 5 cell cultures into 5 wells. | | * Sampled 200 µl from each of the 5 cell cultures into 5 wells. |
| - | * Centrifuged at 1500 rpm, 3 min, in big centrifuge at Wiedner's lab (between E. coli and cell labs). | + | * Centrifuged at 1500 rpm, 3 min, in big centrifuge at the Widmer lab (between E.Coli and cell labs). |
| - | * In Elisa room, transfered 180 µl of supernatant from each sample into row A (columns 1 to 5 correspond to sample #) into new well plate, labeled Test(050712), kept in Elisa freezer (2nd shelf). | + | * In Elisa room, transferred 180 µl of supernatant from each sample into row A (columns 1 to 5 correspond to sample #) into new well plate, labeled Test(050712), kept in Elisa freezer (2nd shelf). |
| - | * Note: from now on, the plate stays out max 10 minutes! To avoid defreezing. | + | * Note: from now on, the plate stays out for max 10 minutes, to avoid defreezing. |
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| | <!-- Note: a list of all protocols can be found here: --> | | <!-- Note: a list of all protocols can be found here: --> |
| | <!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> | | <!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> |
| - | {{:Team:EPF-Lausanne/Template/Protocol|None}}
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| - | ; Comments:
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| - | Insert comments about what happened.
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| | <!-- And here --> | | <!-- And here --> |
| | {{:Team:EPF-Lausanne/Template/Footer}} | | {{:Team:EPF-Lausanne/Template/Footer}} |
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