Team:EPF-Lausanne/Notebook/6 July 2012

(Difference between revisions)

Revision as of 01:09, 23 September 2012

Cell counting.

Supernatant sample stored:

Well plate taken from Elisa room (CHB1374).
Sampled 200 µl from each of the 5 cell cultures into 5 wells.
Centrifuged at 1500 rpm, 3 min, in big centrifuge at the Widmer lab (between E.Coli and cell labs).
In Elisa room, transferred 180 µl of supernatant from each sample into row A (columns 1 to 5 correspond to sample #) into new well plate, labeled Test(050712), kept in Elisa freezer (2nd shelf).
Note: from now on, the plate stays out for max 10 minutes, to avoid defreezing.

@@ Line 15: / Line 15: @@
 * Well plate taken from Elisa room (CHB1374).
 * Sampled 200 µl from each of the 5 cell cultures into 5 wells.
-* Centrifuged at 1500 rpm, 3 min, in big centrifuge at Wiedner's lab (between E. coli and cell labs).
+* Centrifuged at 1500 rpm, 3 min, in big centrifuge at the Widmer lab (between E.Coli and cell labs).
-* In Elisa room, transfered 180 µl of supernatant from each sample into row A (columns 1 to 5 correspond to sample #) into new well plate, labeled Test(050712), kept in Elisa freezer (2nd shelf).
+* In Elisa room, transferred 180 µl of supernatant from each sample into row A (columns 1 to 5 correspond to sample #) into new well plate, labeled Test(050712), kept in Elisa freezer (2nd shelf).
-* Note: from now on, the plate stays out max 10 minutes! To avoid defreezing.
+* Note: from now on, the plate stays out for max 10 minutes, to avoid defreezing.
 <!-- Note: a list of all protocols can be found here: -->
 <!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol  -->
-{{:Team:EPF-Lausanne/Template/Protocol|None}}
-; Comments:
-Insert comments about what happened.
 <!-- And here -->
 {{:Team:EPF-Lausanne/Template/Footer}}