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Team:British Columbia/Interesting Biobricks
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| + | {{Template:Team:British_Columbia_Header}} | ||
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{| border="1" | {| border="1" | ||
|+ Killswitch BioBricks | |+ Killswitch BioBricks | ||
| - | ! | + | ! Numbers !! Team !! Year !! Trigger !! What it Does !! Where? |
| - | ! | + | |
| - | ! | + | |
| - | ! | + | |
| - | ! | + | |
| - | ! | + | |
|- | |- | ||
| <partinfo>BBa_I716462</partinfo> ||UC-Berkeley ||2007|| Arabinose promoter || BamHI ||On Plate 2, 3C| | | <partinfo>BBa_I716462</partinfo> ||UC-Berkeley ||2007|| Arabinose promoter || BamHI ||On Plate 2, 3C| | ||
| Line 72: | Line 69: | ||
| <partinfo>BBa_K108001</partinfo> ||Tsinghua ||2008 || ||Enterobacter phage lysis || | | <partinfo>BBa_K108001</partinfo> ||Tsinghua ||2008 || ||Enterobacter phage lysis || | ||
|} | |} | ||
| + | |||
| + | |||
{| border="1" | {| border="1" | ||
|+ Distribution BioBricks | |+ Distribution BioBricks | ||
| + | ! scope="col" | Location | ||
! scope="col" | Part Name | ! scope="col" | Part Name | ||
! scope="col" | Function | ! scope="col" | Function | ||
| Line 80: | Line 80: | ||
! scope="col" | Other | ! scope="col" | Other | ||
! scope="col" | Plasmid Resistance | ! scope="col" | Plasmid Resistance | ||
| - | |||
|- | |- | ||
| - | | Plate 1 | + | | colspan="6" align="center" | Plate 1 |
|- | |- | ||
| - | | 5P|| <partinfo>BBa_P1003</partinfo>|| Kan Resistance|| Resistance|| Full Cassette|| | + | | 5P|| <partinfo>BBa_P1003</partinfo>|| Kan Resistance|| Resistance|| Full Cassette|| Kan, Amp, Tet |
|- | |- | ||
| - | | 7B|| <partinfo>BBa_P1004</partinfo>|| Chlor Resist|| Resistance|| Full Cassette|| | + | | 7B|| <partinfo>BBa_P1004</partinfo>|| Chlor Resist|| Resistance|| Full Cassette|| Kan, Amp |
|- | |- | ||
| 14C|| <partinfo>BBa_C0261</partinfo>|| AHL Synthase|| AHL Kill Switch|| No Promoter || Amp | | 14C|| <partinfo>BBa_C0261</partinfo>|| AHL Synthase|| AHL Kill Switch|| No Promoter || Amp | ||
| Line 92: | Line 91: | ||
| 24E|| <partinfo>BBa_I13600</partinfo>|| CFP reporter|| fluorescence || 0|| Amp | | 24E|| <partinfo>BBa_I13600</partinfo>|| CFP reporter|| fluorescence || 0|| Amp | ||
|- | |- | ||
| - | | Plate 2 | + | | colspan="6" align="center" | Plate 2 |
|- | |- | ||
| 17F|| <partinfo>BBa_I20260</partinfo>|| GFP reporter|| Fluorescence || Strong const.|| Kan | | 17F|| <partinfo>BBa_I20260</partinfo>|| GFP reporter|| Fluorescence || Strong const.|| Kan | ||
| Line 102: | Line 101: | ||
| 9B|| <partinfo>BBa_J09250</partinfo>|| GFP reporter|| Fluoresence|| Strong Const.|| Kan | | 9B|| <partinfo>BBa_J09250</partinfo>|| GFP reporter|| Fluoresence|| Strong Const.|| Kan | ||
|- | |- | ||
| - | | Plate 3 | + | | colspan="6" align="center" | Plate 3 |
|- | |- | ||
| 2D|| <partinfo>BBa_I746909</partinfo>|| Superfold GFP|| Fluoresence|| T7|| Amp | | 2D|| <partinfo>BBa_I746909</partinfo>|| Superfold GFP|| Fluoresence|| T7|| Amp | ||
| Line 112: | Line 111: | ||
| 13M|| <partinfo>BBa_K093012</partinfo>|| RFP|| Fluorescence|| Strong const.|| Amp | | 13M|| <partinfo>BBa_K093012</partinfo>|| RFP|| Fluorescence|| Strong const.|| Amp | ||
|- | |- | ||
| - | | 14E|| <partinfo>BBa_K081018</partinfo>|| TetR|| Resistance|| Strong const.|| | + | | 14E|| <partinfo>BBa_K081018</partinfo>|| TetR|| Resistance|| Strong const.|| Amp, Kan |
|- | |- | ||
| 15B|| <partinfo>BBa_K112000</partinfo>|| Holin|| Kill Switch|| No promoter|| ?Spec | | 15B|| <partinfo>BBa_K112000</partinfo>|| Holin|| Kill Switch|| No promoter|| ?Spec | ||
| Line 124: | Line 123: | ||
| 20I|| <partinfo>BBa_K124003</partinfo>|| Lysis Cassette|| Kill Switch|| ?|| Kan | | 20I|| <partinfo>BBa_K124003</partinfo>|| Lysis Cassette|| Kill Switch|| ?|| Kan | ||
|- | |- | ||
| - | | 20K|| <partinfo>BBa_K124014</partinfo>|| M S Holin|| Kill Switch|| No Promoter|| | + | | 20K|| <partinfo>BBa_K124014</partinfo>|| M S Holin|| Kill Switch|| No Promoter|| Amp, Kan |
|- | |- | ||
| 20M|| <partinfo>BBa_K124017</partinfo>|| M Lysis cassette|| Kill Switch|| No Promoter|| Kan | | 20M|| <partinfo>BBa_K124017</partinfo>|| M Lysis cassette|| Kill Switch|| No Promoter|| Kan | ||
| Line 130: | Line 129: | ||
| 21O|| <partinfo>BBa_K106674</partinfo>|| GFP|| Fluorescence|| Med. Const.|| ?amp | | 21O|| <partinfo>BBa_K106674</partinfo>|| GFP|| Fluorescence|| Med. Const.|| ?amp | ||
|- | |- | ||
| - | | 22A|| <partinfo>BBa_K131000</partinfo>|| Colicin E2|| Kill Switch|| SOS promoter|| | + | | 22A|| <partinfo>BBa_K131000</partinfo>|| Colicin E2|| Kill Switch|| SOS promoter|| Kan, Amp |
|- | |- | ||
| - | | 22C|| <partinfo>BBa_K131010</partinfo>|| Colicin E2|| Kill Switch|| | + | | 22C|| <partinfo>BBa_K131010</partinfo>|| Colicin E2|| Kill Switch|| AHL induce, c GFP || "Kan, Amp |
|- | |- | ||
| - | | 24E|| <partinfo>BBa_K112022</partinfo>|| Lysis Device || Kill Switch|| | + | | 24E|| <partinfo>BBa_K112022</partinfo>|| Lysis Device || Kill Switch|| No Prom, weak anti protein || Amp, Kan |
|- | |- | ||
| - | | Plate 4 | + | | colspan="6" align="center" | Plate 4 |
|- | |- | ||
| 3I|| <partinfo>BBa_K398406</partinfo>|| Solvent Tolerant|| Other (Hex. Tol)|| Strong const.|| Chlor | | 3I|| <partinfo>BBa_K398406</partinfo>|| Solvent Tolerant|| Other (Hex. Tol)|| Strong const.|| Chlor | ||
| Line 148: | Line 147: | ||
| 21G|| <partinfo>BBa_K314203</partinfo>|| Tse Tox. Ind.Anti|| Kill switch|| HSL ind immunity|| Chlor | | 21G|| <partinfo>BBa_K314203</partinfo>|| Tse Tox. Ind.Anti|| Kill switch|| HSL ind immunity|| Chlor | ||
|- | |- | ||
| - | | Plate 5 | + | | colspan="6" align="center" | Plate 5 |
|- | |- | ||
| 1A|| <partinfo>BBa_K515107</partinfo>|| Photo-Switch|| Death test/Fluor|| pTet|| Chlor | | 1A|| <partinfo>BBa_K515107</partinfo>|| Photo-Switch|| Death test/Fluor|| pTet|| Chlor | ||
Latest revision as of 04:05, 16 June 2012
| Numbers | Team | Year | Trigger | What it Does | Where? |
|---|---|---|---|---|---|
| <partinfo>BBa_I716462</partinfo> | UC-Berkeley | 2007 | Arabinose promoter | BamHI | |
| <partinfo>BBa_K512002</partinfo> | USC | 2011 | IPTG, targets GFP gene | CRISPR-mediated GFP plasmid degradation | |
| <partinfo>BBa_K112808</partinfo> | UC-Berkeley | 2008 | No Promoter | T4 Lysis device | Plate 3, 6G |
| <partinfo>BBa_K541545</partinfo> | FAGEM | 2011 | IPTG | Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible) | |
| <partinfo>BBa_K593009</partinfo> | METU-Ankara | 2011 | T7 Promoter | ROSE regulated kill switch, (RNA thermometer, only activates above 42°) | |
| <partinfo>BBa_K628006</partinfo> | St. Andrews | 2011 | pBad | Protegrin-1 Kill Switch, (Antimicrobial peptide) | |
| Strasbourg | 2010 | I don't know what the heck. | |||
| Tokyo Metropolitan | 2011 | Killer Bee e.coli? | |||
| UNIST Korea | 2011 | Killed by high and low temps, not if dark. | |||
| Harvard | 2010 | Barnase cleaves RNA! | |||
| <partinfo>BBa_I716211</partinfo> | UC Berkeley | 2007 | No Promoter | Harvard got IPTG inducible Barnase/Barnstar from Anderson Lab at UCB | |
| Hong Kong | 2010 | Possible new killswitch, not implemented | |||
| <partinfo>BBa_K565001</partinfo> | Valencia | 2011 | ? 12 kb | Colicin G | |
| <partinfo>BBa_K565002</partinfo> | Valencia | 2011 | ? 12 kb | Colicin H | |
| <partinfo>BBa_K565004</partinfo> | Valencia | 2011 | No Promoter | Microcin C51 production device (5 restriction sites) | |
| <partinfo>BBa_K185004</partinfo> | SJTU-BioX-Shanghai | 2009 | No Promoter | RelE toxin+Double terminator | |
| UNICAMP Brazil | 2009 | All sorts of kill switches, but may not all work in E. coli. | |||
| <partinfo>BBa_K284017</partinfo> | UNICAMP-Brazil | 2009 | Lactate (Yeast) | Lysozyme | |
| <partinfo>BBa_K131000</partinfo> | Calgary | 2008 | SOS Promoter | Colicin 2E | 3, 22A |
| <partinfo>BBa_K112806</partinfo> | UC Berkeley | 2008 | T7 | T4 Endolysin | |
| <partinfo>BBa_K302035</partinfo> | Newcastle | 2010 | Sucrose limit | MazEF | |
| <partinfo>BBa_K124014</partinfo> | Brown | 2008 | No Promoter | Mutated Holin Gene (Inconsistent sequencing) | |
| <partinfo>BBa_K419003</partinfo> | TzuChiU_Formosa | 2010 | pLys | Lysis Cassette - no antiholin | |
| <partinfo>BBa_K317039</partinfo> | Tokyo-NoKoGen | 2010 | AHL | AHL sensitive lysis cassette | |
| <partinfo>BBa_K317040</partinfo> | Tokyo-NoKoGen | 2010 | IPTG | IPTG sensitive lysis cassette | |
| <partinfo>BBa_K299806</partinfo> | Warsaw | 2010 | MinC cell division inhibitor | ||
| <partinfo>BBa_K150007</partinfo> | Heidelberg | 2008 | Native Operon | Colicin E1 | |
| <partinfo>BBa_K150010</partinfo> | Heidelberg | 2008 | Native Operon | Colicin E9 | |
| <partinfo>BBa_K145230</partinfo> | KULeuven | 2008 | HSL-LuxR | ccdb | |
| <partinfo>BBa_K117009</partinfo> | NTU-Singapore | 2008 | IPTG | Colicin E7 | |
| <partinfo>BBa_K117000</partinfo> | NTU-Singapore | 2008 | Lysin (activates Colicin E7) | ||
| <partinfo>BBa_K108001</partinfo> | Tsinghua | 2008 | Enterobacter phage lysis |
| Location | Part Name | Function | Use | Other | Plasmid Resistance |
|---|---|---|---|---|---|
| Plate 1 | |||||
| 5P | <partinfo>BBa_P1003</partinfo> | Kan Resistance | Resistance | Full Cassette | Kan, Amp, Tet |
| 7B | <partinfo>BBa_P1004</partinfo> | Chlor Resist | Resistance | Full Cassette | Kan, Amp |
| 14C | <partinfo>BBa_C0261</partinfo> | AHL Synthase | AHL Kill Switch | No Promoter | Amp |
| 24E | <partinfo>BBa_I13600</partinfo> | CFP reporter | fluorescence | 0 | Amp |
| Plate 2 | |||||
| 17F | <partinfo>BBa_I20260</partinfo> | GFP reporter | Fluorescence | Strong const. | Kan |
| 14N | <partinfo>BBa_K117009</partinfo> | Colicin E7 | Kill Switch | Lactose prom | Amp |
| 14D | <partinfo>BBa_K117000</partinfo> | Lysis gene | Kill Switch | Aids colicin | Amp |
| 9B | <partinfo>BBa_J09250</partinfo> | GFP reporter | Fluoresence | Strong Const. | Kan |
| Plate 3 | |||||
| 2D | <partinfo>BBa_I746909</partinfo> | Superfold GFP | Fluoresence | T7 | Amp |
| 5G | <partinfo>BBa_K137076</partinfo> | H2O2 gen. | Kill Switch | AHL induce | Amp |
| 6G | <partinfo>BBa_K112808</partinfo> | Lysis Device | Kill Switch | No Promoter | Amp |
| 13M | <partinfo>BBa_K093012</partinfo> | RFP | Fluorescence | Strong const. | Amp |
| 14E | <partinfo>BBa_K081018</partinfo> | TetR | Resistance | Strong const. | Amp, Kan |
| 15B | <partinfo>BBa_K112000</partinfo> | Holin | Kill Switch | No promoter | ?Spec |
| 15D | <partinfo>BBa_K112005</partinfo> | "RBS, Holin" | Kill Switch | No Promoter | ?Spec |
| 15H | <partinfo>BBa_k112017</partinfo> | lysozyme | Kill Switch | No Promoter | ?Spec |
| 15J | <partinfo>BBa_K112106</partinfo> | BgIII endonuc. | Kill Switch | No Promoter | ?Spec |
| 20I | <partinfo>BBa_K124003</partinfo> | Lysis Cassette | Kill Switch | ? | Kan |
| 20K | <partinfo>BBa_K124014</partinfo> | M S Holin | Kill Switch | No Promoter | Amp, Kan |
| 20M | <partinfo>BBa_K124017</partinfo> | M Lysis cassette | Kill Switch | No Promoter | Kan |
| 21O | <partinfo>BBa_K106674</partinfo> | GFP | Fluorescence | Med. Const. | ?amp |
| 22A | <partinfo>BBa_K131000</partinfo> | Colicin E2 | Kill Switch | SOS promoter | Kan, Amp |
| 22C | <partinfo>BBa_K131010</partinfo> | Colicin E2 | Kill Switch | AHL induce, c GFP | "Kan, Amp |
| 24E | <partinfo>BBa_K112022</partinfo> | Lysis Device | Kill Switch | No Prom, weak anti protein | Amp, Kan |
| Plate 4 | |||||
| 3I | <partinfo>BBa_K398406</partinfo> | Solvent Tolerant | Other (Hex. Tol) | Strong const. | Chlor |
| 6M | <partinfo>BBa_K112806</partinfo> | T4 Endolysin | Kill Switch | No Prom | Amp |
| 7C | <partinfo>BBa_K314200</partinfo> | Tse Toxin | Kill Switch | No Prom | Chlor |
| 8G | <partinfo>BBa_K206000</partinfo> | pBAD Promoter | Promoter | Arabinose ind | Amp |
| 21G | <partinfo>BBa_K314203</partinfo> | Tse Tox. Ind.Anti | Kill switch | HSL ind immunity | Chlor |
| Plate 5 | |||||
| 1A | <partinfo>BBa_K515107</partinfo> | Photo-Switch | Death test/Fluor | pTet | Chlor |
| 12O | <partinfo>BBa_I13604</partinfo> | Con. YFP ind CFP | Fluor | Strong const./lacI | Amp |
| 14I | <partinfo>BBa_K299806</partinfo> | minC | Kill Switch | No Promoter | Amp |
