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Team:Wageningen UR/Journal/week13
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The figure below shows the purity of isolated RNA. We isolated RNA of different potato cultivars. | The figure below shows the purity of isolated RNA. We isolated RNA of different potato cultivars. | ||
| - | [[File:rna2.jpg]] | + | [[File:rna2.jpg|frame|center|Figure 1:...]] |
| - | [[File:table1.jpg]] | + | [[File:table1.jpg|frame|center|Figure 2: ...]] |
We performed Reverse Transciptase reactions followed by PCR. We used the Revert AID H Minus Reverse Transcription Kit (Fermentas). | We performed Reverse Transciptase reactions followed by PCR. We used the Revert AID H Minus Reverse Transcription Kit (Fermentas). | ||
| - | [[File:rt-pcr.jpg]] | + | [[File:rt-pcr.jpg|frame|center|Figure 3: ...]] |
Agarose gel of PCR products. Lanes 1 till 4 show the PLRV Coat Protein (CP) bands with the expected size of 627 bp. Lanes 6 till 9 show the PLRV Coat Protein plus Read Through part (CP_RT) with the expected size of 2154 bp. | Agarose gel of PCR products. Lanes 1 till 4 show the PLRV Coat Protein (CP) bands with the expected size of 627 bp. Lanes 6 till 9 show the PLRV Coat Protein plus Read Through part (CP_RT) with the expected size of 2154 bp. | ||
Revision as of 09:34, 21 September 2012
week 13: 23 july - 29 july
Not much work was done this week because the team spent four days on going to a three day synthetic biology conference in Munich. We learned a lot there and had a great time!
Office work
Lab work
PLRV
- RNA isolation of potato leaf tissue
- Reverse transcriptase reaction to obtain cDNA
- PCR on cDNA using coat protein primers
The figure below shows the purity of isolated RNA. We isolated RNA of different potato cultivars.
Figure 1:...
Figure 2: ...
We performed Reverse Transciptase reactions followed by PCR. We used the Revert AID H Minus Reverse Transcription Kit (Fermentas).
Figure 3: ...
Agarose gel of PCR products. Lanes 1 till 4 show the PLRV Coat Protein (CP) bands with the expected size of 627 bp. Lanes 6 till 9 show the PLRV Coat Protein plus Read Through part (CP_RT) with the expected size of 2154 bp.
