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Team:TU Darmstadt/Materials/Chloramphenicol
From 2012.igem.org
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#* 1 g chloramphenicol (25 mg/mL) | #* 1 g chloramphenicol (25 mg/mL) | ||
#* ad. 40 mL ethanol | #* ad. 40 mL ethanol | ||
| - | + | # aliqout in 1 mL stocks and store at -20°C | |
=== Stability === | === Stability === | ||
Revision as of 18:15, 16 September 2012
Contents |
Chloramphenicol
About
Chloramphenicol is a bacteriostatic antibiotic that specifically binds resudies in the 50S ribosomal subunit, preventing peptide bond formation.[1]
Stock
- Mix:
- 1 g chloramphenicol (25 mg/mL)
- ad. 40 mL ethanol
- aliqout in 1 mL stocks and store at -20°C
Stability
- Chloramphenicol is not autoclavable, it has to be added before pouring plates (keep in mind that the agar is not too hot when adding it)
References
[1] http://en.wikipedia.org/wiki/Chloramphenicol
