Team:UNITN-Trento/Parts

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<h2 style="text-align: center;">Our Parts</h2>
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<p>Here you can find our favourite Parts and all the Parts we built. Make sure to check out our <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=UNITN-Trento">Team Parts Page on the Registry website</a>!</p>
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<hr style="width: 98%;">
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<h2 style="text-align: center;">FAVORITES</h2>
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<div class="favPart" style="border-left: 7px solid #50aae4; padding-left: 10px;">
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<div class="star"></div>
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">K731030 - Inducible araC-pBAD promoter regulating M256I CysE</a></h2>
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<p>A serine acetyltransferase (CysE) controlled by an arabinose inducible system. <br/>
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This is a key part in our black crust project: a device used to reduce sulfate and produce cysteine. </p>
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<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>
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</div>
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<div class="favPart" style="border-left: 7px solid #67f853; padding-left: 10px;">
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<div class="star"></div>
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">K731400 - IPTG inducible Cysteine desulfhydrase (CysDes)</a></h2>
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<p>Our favorite BioBrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key part for the Crustaway project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid.</p>
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<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>
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</div>
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<div class="favPart" style="border-left: 7px solid #ffae00; padding-left: 10px;">
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<div class="star"></div>
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<h2 class="orange"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731721">K731721 - T7 terminator</a></h2>
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<p>Our new entry in the terminator collections of the registry: a T7 terminator! This terminator was characterized in vivo and in vitro with both T7 and E. coli polymerases. </p>
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<a class="button" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030" style="text-decoration: none;">Registry Page</a>
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</div>
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<hr>
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<h2 style="text-align: center;"> All Our Parts</h2>
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<p>Please note that many parts have been also subcloned into low copy vectors. Although these parts have note been submitted to the registry, they are available upon request. Contact us at igemtrento@gmail.com.</p>
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<h3>The Black Crust Enzymes</h3>
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<div class="Part blue">
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731000">K731000 CysE (Serine Acetyltransferase)</a></h2>
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<p>A part encoding CysE, our key enzyme in the reduction of sulfate.</p>
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</div>
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<div class="Part blue">
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731010">K731010 M256I CysE (Serine Acetyltransferase)</a></h2>
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<p>This is a mutated CysE. Introduction of a Methionine at position 256, makes our key player even "stronger" and enhances sulfate uptake.
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</p>
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</div>
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<div class="Part blue">
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731020">K731020 Inducible araC-pBAD promoter regulating WT CysE</a></h2>
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<p>TA device to produce CysE upon induction with arabinose.  Also available in pSB3C5.
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</p>
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</div>
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<div class="Part blue" >
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">K731030 Inducible araC-pBAD promoter regulating M256I CysE</a></h2>
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<p>A device for the production of M256I CysE controlled by an arabinose inducible cassette. This part was used for the bioremoval of the black crust from different stones samples. Also available in pSB3C5.</p>
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</div>
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<div class="Part blue">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731040">K731040 Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE</a></h2>
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<p>This is our favorite part BBa_K731030 followed by a sfGFP. The device was created by Gibson assembly.
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</p>
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<div class="punch" style="margin: 0 auto;">
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<h2 style="text-align: center; font-size: 25px;">Terminator 5</h2>
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</div>
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<hr style="width: 100%; margin-top: 10px; margin-bottom: 30px;">
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<div class="Part green" >
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">K731400 IPTG inducible Cysteine desulfhydrase (CysDes)</a></h2>
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<div id="selector">
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<p>Our favorite biobrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key part for the Crustaway project. This biobrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid. Also available in pSB4K5.</p>
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<div id="intro" class="but now"><img src="http://www.science.unitn.it/~igem/img/project/buttons/button_13.png" alt="" /><span class="title">Introduction</span></div>
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</div>
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<div id="method" class="but"><img src="http://www.science.unitn.it/~igem/img/project/buttons/button_10.png" alt="" /><span class="title">Our Method</span></div>
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<div id="results" class="but"><img src="http://www.science.unitn.it/~igem/img/project/buttons/button_11.png" alt="" /><span class="title">Results</span></div>
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<div class="Part green">
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<div id="application" class="but"><img  src="http://www.science.unitn.it/~igem/img/project/buttons/button_12.png" alt="" /><span class="title">Future</span></div>
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731480">K731480 IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)</a></h2>
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<div  class="but"><img  src="http://www.science.unitn.it/~igem/img/project/buttons/button_1.png" alt="" /><span class="title">Crust Away</span></div>
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<p>This is a sfGFP tagged version of our best biobrick BBa_K731400. A superfluo device created by Gibson assembly. Also available in pSB4K5.
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</p>
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<div class="clearfix"></div>
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<div class="content" style="padding-left: 20px ;padding-right: 20px ;">
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<div id="intro" class="chapter intro visible">
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<h3>INTRO</h3>
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<ul>
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<li>2nd: GET TOGETHER!! Cristina brought brownies!</li>
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<h3>Terminators</h3>
-
<li>9th: We started looking at some of the previous successful projects.</li>
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-
<li>16th: More on the old projects eating a fantastic Tiramisu from Giacomo.</li>
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<div class="Part orange">
-
<li>23rd: Crash course on Synthetic Biology from Sheref and Cristina</li>
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731721">K731721 T7 terminator</a></h2>
-
</ul>
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<p>our new entry in the terminator collections of the registry: a T7 terminator! This terminator was characterized in vivo and in vitro with both T7 and E. coli polymerases. </p>
 +
</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">K731722 B0010 E. coli terminator</a></h2>
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<p>A good working alternative for the E.coli terminator B0010. </p>
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</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">K731700 Platform for terminators analysis under the control of T7 promoter</a></h2>
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<p>Our measurement device for terminators characterization. A T7 promoter controlling the expression of A206K Venus and mCherry for the ratiometric analysis of terminator efficiency.</p>
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</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731710">K731710 Platform for terminators analysis under the control of TAC promoter</a></h2>
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<p>Our great platform BBa_K731722, where T7 promoter was substituted with Ptac.</p>
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</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731701">K731701 BBa_K731700 with T7terminator (BBa_K731721)</a></h2>
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<p>A measurement device used to characterize T7 terminator under the control of T7 promoter.</p>
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</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731702">K731702 BBa_K731700 with BBa_K731722 terminator (BBa_B0010)</a></h2>
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<p>A measurement device used to characterize B0010 terminator under the control of T7 promoter.</p>
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</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731711">K731711 BBa_K731710 with T7 terminator (BBa_K731721)</a></h2>
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<p>A measurement device used to characterize the T7 terminator (BBa_K731721) under the control of tac promoter.</p>
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</div>
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<div class="Part orange">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731712">K731712 BBa_K731710 with BBa_K731722 terminator (BBa_B0010)</a></h2>
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<p>A measurement device used to characterize the E.coli terminator (BBa_K731722) under the control of tac promoter.</p>
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</div>
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<h3>Promoters</h3>
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<h3>APRIL</h3>
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<div class="Part blue">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731201">K731201 Inducible araC-pBAD promoter</a></h2>
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<ul>
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<p>A new version of the araC-pBad promoter that was amplified from the E.coli genome. An excellent alternative to BBa_I0050.</p>
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<li>13rd: Brainstorming. No more cakes?</li>
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</div>
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<li>16th: Brainstorming. Where is the cake?</li>
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-
<li>19th: It seems like everybody likes the idea proposed by Daniele. Remove the black crust from statues. We decided to look more specifically into these topics: A) how to dissolve the black crust, B) how to keep the &#8220;patina nobile&#8221; (calcium oxalate protective film) and C) how to kill biofilms, all to protect statues and marble monuments. Giacomo will look into secretion systems and Francesco suggested a photoinducible system. We have homework to do.</li>
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<div class="Part green">
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<li>24th: Discussion on possible ways to achieve our goals. We looked into the mechanism of SRB for sulfate reduction.</li>
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731300">K731300 LacI + LacIq promoter (reverse)</a></h2>
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<li>27th: More ideas on the Black crust project based on a work of J.Keasling that Jason found in the literature. We are also thinking of having a side project for the characterization of deposited parts (RBS, promoters, terminators?). Each of us will further investigate the fesibility of the topics discussed on April 19th and will present a plan of work for next time.</li>
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<p>A ready to go IPTG inducible cassette that is composed of LacI controlled by the lacIq promoter, both in the reverse direction.</p>
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</ul>
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</div>
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<h3>MAY</h3>
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<div class="Part green">
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731500">K731500 [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward</a></h2>
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<ul>
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<p>Our part BBa_K731300 followed by a tac promoter and a lac operator. The perfect biobrick for those interested in expressing their gene under the control of IPTG. It will save you a lot of cloning steps! Also available in pSB4K5.
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<li>14th: Presentations and discussion on the investigated topics. Other PIs from CIBIO participated to the discussion too.</li>
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</p>
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<li>28th: The project is finalized: We will save the world one statue at time! We decided to drop the project on the formation of calcium oxalate and biofilm disruption. Perhaps next year team can look into it.</li>
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</div>
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</ul>
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</div>
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<div class="Part blue">
-
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731250">K731250 Inducible araC-pBAD promoter with GFPmut3b, strong RBS</a></h2>
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<div class="chapter method">
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<p>A device for the characterization of our new entry araC-pBad (BBa_K731201) followed by a strong RBS and a GFPmut3b. Also available in pSB3C5.</p>
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<h3>METHOD</h3>
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</div>
-
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<ul>
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<div class="Part blue" >
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<li>2nd: GET TOGETHER!! Cristina brought brownies!</li>
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<h2 ><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731255">K731255 Inducible araC-pBAD promoter with GFPmut3b, weak RBS</a></h2>
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<li>9th: We started looking at some of the previous successful projects.</li>
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<p>A device for the characterization of our new entry araC-pBad (BBa_K731201) followed by a weak RBS and GFPmut3b.</p>
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<li>16th: More on the old projects eating a fantastic Tiramisu from Giacomo.</li>
+
</div>
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<li>23rd: Crash course on Synthetic Biology from Sheref and Cristina</li>
+
-
</ul>
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<div class="Part green">
-
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731520">K731520 IPTG inducible GFPmut3b</a></h2>
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<h3>APRIL</h3>
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<p>A device for the characterization of our new BBa_K731500 followed by a strong RBS and GFPmut3b. Also available in pSB4K5.</p>
-
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</div>
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<ul>
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-
<li>13rd: Brainstorming. No more cakes?</li>
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</div> <!--end content-->
-
<li>16th: Brainstorming. Where is the cake?</li>
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</section><!-- end main-->
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<li>19th: It seems like everybody likes the idea proposed by Daniele. Remove the black crust from statues. We decided to look more specifically into these topics: A) how to dissolve the black crust, B) how to keep the &#8220;patina nobile&#8221; (calcium oxalate protective film) and C) how to kill biofilms, all to protect statues and marble monuments. Giacomo will look into secretion systems and Francesco suggested a photoinducible system. We have homework to do.</li>
+
-
<li>24th: Discussion on possible ways to achieve our goals. We looked into the mechanism of SRB for sulfate reduction.</li>
+
-
<li>27th: More ideas on the Black crust project based on a work of J.Keasling that Jason found in the literature. We are also thinking of having a side project for the characterization of deposited parts (RBS, promoters, terminators?). Each of us will further investigate the fesibility of the topics discussed on April 19th and will present a plan of work for next time.</li>
+
-
</ul>
+
-
+
-
<h3>MAY</h3>
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-
+
-
<ul>
+
-
<li>14th: Presentations and discussion on the investigated topics. Other PIs from CIBIO participated to the discussion too.</li>
+
-
<li>28th: The project is finalized: We will save the world one statue at time! We decided to drop the project on the formation of calcium oxalate and biofilm disruption. Perhaps next year team can look into it.</li>
+
-
</ul>
+
-
</div>
+
-
+
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<div class="chapter results">
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<h3>RESULTS</h3>
+
-
+
-
<ul>
+
-
<li>2nd: GET TOGETHER!! Cristina brought brownies!</li>
+
-
<li>9th: We started looking at some of the previous successful projects.</li>
+
-
<li>16th: More on the old projects eating a fantastic Tiramisu from Giacomo.</li>
+
-
<li>23rd: Crash course on Synthetic Biology from Sheref and Cristina</li>
+
-
</ul>
+
-
+
-
<h3>APRIL</h3>
+
-
+
-
<ul>
+
-
<li>13rd: Brainstorming. No more cakes?</li>
+
-
<li>16th: Brainstorming. Where is the cake?</li>
+
-
<li>19th: It seems like everybody likes the idea proposed by Daniele. Remove the black crust from statues. We decided to look more specifically into these topics: A) how to dissolve the black crust, B) how to keep the &#8220;patina nobile&#8221; (calcium oxalate protective film) and C) how to kill biofilms, all to protect statues and marble monuments. Giacomo will look into secretion systems and Francesco suggested a photoinducible system. We have homework to do.</li>
+
-
<li>24th: Discussion on possible ways to achieve our goals. We looked into the mechanism of SRB for sulfate reduction.</li>
+
-
<li>27th: More ideas on the Black crust project based on a work of J.Keasling that Jason found in the literature. We are also thinking of having a side project for the characterization of deposited parts (RBS, promoters, terminators?). Each of us will further investigate the fesibility of the topics discussed on April 19th and will present a plan of work for next time.</li>
+
-
</ul>
+
-
+
-
<h3>MAY</h3>
+
-
+
-
<ul>
+
-
<li>14th: Presentations and discussion on the investigated topics. Other PIs from CIBIO participated to the discussion too.</li>
+
-
<li>28th: The project is finalized: We will save the world one statue at time! We decided to drop the project on the formation of calcium oxalate and biofilm disruption. Perhaps next year team can look into it.</li>
+
-
</ul>
+
-
</div>
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-
+
-
<div class="chapter application">
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<h3>APPLICATION</h3>
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<li>2nd: GET TOGETHER!! Cristina brought brownies!</li>
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<li>9th: We started looking at some of the previous successful projects.</li>
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<li>16th: More on the old projects eating a fantastic Tiramisu from Giacomo.</li>
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<li>23rd: Crash course on Synthetic Biology from Sheref and Cristina</li>
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<li>13rd: Brainstorming. No more cakes?</li>
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<li>16th: Brainstorming. Where is the cake?</li>
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<li>19th: It seems like everybody likes the idea proposed by Daniele. Remove the black crust from statues. We decided to look more specifically into these topics: A) how to dissolve the black crust, B) how to keep the &#8220;patina nobile&#8221; (calcium oxalate protective film) and C) how to kill biofilms, all to protect statues and marble monuments. Giacomo will look into secretion systems and Francesco suggested a photoinducible system. We have homework to do.</li>
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<li>24th: Discussion on possible ways to achieve our goals. We looked into the mechanism of SRB for sulfate reduction.</li>
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<li>27th: More ideas on the Black crust project based on a work of J.Keasling that Jason found in the literature. We are also thinking of having a side project for the characterization of deposited parts (RBS, promoters, terminators?). Each of us will further investigate the fesibility of the topics discussed on April 19th and will present a plan of work for next time.</li>
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<h3>MAY</h3>
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<li>14th: Presentations and discussion on the investigated topics. Other PIs from CIBIO participated to the discussion too.</li>
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<li>28th: The project is finalized: We will save the world one statue at time! We decided to drop the project on the formation of calcium oxalate and biofilm disruption. Perhaps next year team can look into it.</li>
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Revision as of 17:14, 16 September 2012

Our Parts

Our Parts

Here you can find our favourite Parts and all the Parts we built. Make sure to check out our Team Parts Page on the Registry website!

FAVORITES

K731030 - Inducible araC-pBAD promoter regulating M256I CysE

A serine acetyltransferase (CysE) controlled by an arabinose inducible system.
This is a key part in our black crust project: a device used to reduce sulfate and produce cysteine.

Registry Page

K731400 - IPTG inducible Cysteine desulfhydrase (CysDes)

Our favorite BioBrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key part for the Crustaway project. This BioBrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid.

Registry Page

K731721 - T7 terminator

Our new entry in the terminator collections of the registry: a T7 terminator! This terminator was characterized in vivo and in vitro with both T7 and E. coli polymerases.

Registry Page

All Our Parts

Please note that many parts have been also subcloned into low copy vectors. Although these parts have note been submitted to the registry, they are available upon request. Contact us at igemtrento@gmail.com.

The Black Crust Enzymes

K731000 CysE (Serine Acetyltransferase)

A part encoding CysE, our key enzyme in the reduction of sulfate.

K731010 M256I CysE (Serine Acetyltransferase)

This is a mutated CysE. Introduction of a Methionine at position 256, makes our key player even "stronger" and enhances sulfate uptake.

K731020 Inducible araC-pBAD promoter regulating WT CysE

TA device to produce CysE upon induction with arabinose. Also available in pSB3C5.

K731030 Inducible araC-pBAD promoter regulating M256I CysE

A device for the production of M256I CysE controlled by an arabinose inducible cassette. This part was used for the bioremoval of the black crust from different stones samples. Also available in pSB3C5.

K731040 Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE

This is our favorite part BBa_K731030 followed by a sfGFP. The device was created by Gibson assembly.

K731400 IPTG inducible Cysteine desulfhydrase (CysDes)

Our favorite biobrick encoding a cysteine desulfhydrase, controlled by an IPTG inducible cassette. This is the second key part for the Crustaway project. This biobrick produces an enzyme that uses cysteine as the substrate to form sulfidric acid. Also available in pSB4K5.

K731480 IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)

This is a sfGFP tagged version of our best biobrick BBa_K731400. A superfluo device created by Gibson assembly. Also available in pSB4K5.

Terminators

K731721 T7 terminator

our new entry in the terminator collections of the registry: a T7 terminator! This terminator was characterized in vivo and in vitro with both T7 and E. coli polymerases.

K731722 B0010 E. coli terminator

A good working alternative for the E.coli terminator B0010.

K731700 Platform for terminators analysis under the control of T7 promoter

Our measurement device for terminators characterization. A T7 promoter controlling the expression of A206K Venus and mCherry for the ratiometric analysis of terminator efficiency.

K731710 Platform for terminators analysis under the control of TAC promoter

Our great platform BBa_K731722, where T7 promoter was substituted with Ptac.

K731701 BBa_K731700 with T7terminator (BBa_K731721)

A measurement device used to characterize T7 terminator under the control of T7 promoter.

K731702 BBa_K731700 with BBa_K731722 terminator (BBa_B0010)

A measurement device used to characterize B0010 terminator under the control of T7 promoter.

K731711 BBa_K731710 with T7 terminator (BBa_K731721)

A measurement device used to characterize the T7 terminator (BBa_K731721) under the control of tac promoter.

K731712 BBa_K731710 with BBa_K731722 terminator (BBa_B0010)

A measurement device used to characterize the E.coli terminator (BBa_K731722) under the control of tac promoter.

Promoters

K731201 Inducible araC-pBAD promoter

A new version of the araC-pBad promoter that was amplified from the E.coli genome. An excellent alternative to BBa_I0050.

K731300 LacI + LacIq promoter (reverse)

A ready to go IPTG inducible cassette that is composed of LacI controlled by the lacIq promoter, both in the reverse direction.

K731500 [LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward

Our part BBa_K731300 followed by a tac promoter and a lac operator. The perfect biobrick for those interested in expressing their gene under the control of IPTG. It will save you a lot of cloning steps! Also available in pSB4K5.

K731250 Inducible araC-pBAD promoter with GFPmut3b, strong RBS

A device for the characterization of our new entry araC-pBad (BBa_K731201) followed by a strong RBS and a GFPmut3b. Also available in pSB3C5.

K731255 Inducible araC-pBAD promoter with GFPmut3b, weak RBS

A device for the characterization of our new entry araC-pBad (BBa_K731201) followed by a weak RBS and GFPmut3b.

K731520 IPTG inducible GFPmut3b

A device for the characterization of our new BBa_K731500 followed by a strong RBS and GFPmut3b. Also available in pSB4K5.

Retrieved from "http://2012.igem.org/Team:UNITN-Trento/Parts"