Team:SDU-Denmark/labwork/Protocols/3A

From 2012.igem.org

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<h1> 3A assembly </h1>
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<h2> Ligation of 2 parts into a vector</h2>
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<h3> Ligation of 2 parts into a vector</h3>
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Revision as of 20:14, 15 September 2012

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID


mRNA Isolation PCR Miniprep Check Digest
3A-Assembly Content Content Content
Content Content Content Content

3A assembly

Ligation of 2 parts into a vector

  1. PCR step; see PCR protocol
  2. Digestion
    3. BSA is only needed for conventional restriction enzymes, NOT fast digest
    1. Digest step for Plasmid Backbone

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